Carlina species as a new source of bioactive pentacyclic triterpenes

Strzemski, Maciej; Wójciak–Kosior, Magdalena; Sowa, Ireneusz; Rutkowska, Ewelina; Szwerc, Wojciech; Kocjan, Ryszard; Latalski, M

doi:10.1016/j.indcrop.2016.09.025

Cited by 39 publications

(35 citation statements)

References 29 publications

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“…The content of UA and OA in the leaves of such plants was approx. 2.8 mg•g −1 DW and 0.5 mg•g −1 DW, respectively [6]. Our current study showed that the content of these compounds in the leaves of the field crops reached 2.05 mg•g−1 DW and 5.57 mg•g −1 DW of OA and UA, respectively.…”

Section: Hplc-pda Analysissupporting

confidence: 52%

“…acaulis is a valuable plant with confirmed biological activity. Our previous investigation revealed the presence of high amounts of ChA, OA, UA, and CO [6,8,12,20]. However, due to the limited occurrence of C. acaulis and protection of this species, collection of plant material from natural sites is difficult; thus, the search for alternative approaches is important for the widespread use of this plant.…”

Section: Resultsmentioning

confidence: 99%

“…The content of OA ranged from 0.03 (TRD line: callus obtained from roots of plants with tomentose leaves growing on medium D) to 0.23 (GLE lines: callus obtained from leaves of plants with glabrous leaves growing on medium E) mg•g −1 DW, and UA was in the range from 0.03 (GRC line) to 0.29 (GLE line) mg•g −1 DW. Although only low amounts of triterpenic acids were found in the callus tissues obtained from the roots, their occurrence is interesting given the absence of these compounds in the roots of plants from the natural state [6]. The reduction of the UA and OA concentration in comparison to naturally growing plants is characteristic for the in vitro model [27][28][29].…”

Section: Hplc-pda Analysismentioning

confidence: 99%

“…The compounds were separated by gradient elution with the following program: 0-25 min A 8%, B 92%; 25-60 min A 8-37%, B 92-63%. The flow rate was 1 mL/min and the temperature of the thermostat was 25 • C. Triterpenic acids were determined in an isocratic system according to the methodology published previously [6]. A mixture of ACN, water, and phosphoric acid aqueous solution at a concentration of 1% (75:25:0.5, v/v/v) was used as a mobile phase.…”

Section: Hplc-pda Analysismentioning

confidence: 99%

“…Both roots and aboveground parts of the plant are used as diaphoretic, diuretic, anthelmintic, and antibacterial drugs in ethnomedicine in Italy [2,3], Montenegro, Bosnia and Herzegovina, Serbia [4], and Macedonia [5]. The biological activity of extracts from Carlina plants is associated with the presence of such compounds as pentacyclic triterpenes: oleanolic (OA) and ursolic acids (UA) [6], chlorogenic acid (ChA), and 3,5-di-caffeoylquinic acid (3,5CQA) [7,8]; flavonoids: vitexin, orientin, homoorientin and schaftoside [9,10]; and carlina oxide (CO) [10][11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

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The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of Carlina acaulis Plant Material

et al. 2019

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Roots and leaves of Carlina acaulis L. are still used in ethnomedicine in many European countries; however, the limited occurrence of the plants and protection of this species necessitate a search for alternative ways for obtaining this plant material. In this study, in vitro cultures, hydroponic cultures, and field cultivation were applied to obtain the C. acaulis plant material. Its quality was evaluated using antioxidant activity tests and high performance liquid chromatography analysis. Our study showed that the antioxidant activity and the content of chlorogenic and 3,5-di-caffeoylquinic acid in roots of plants cultivated in hydroponics and field conditions were comparable. However, the amount of carlina oxide was significantly higher in plants from the field. The flavonoid content in leaves obtained from both cultivation systems was at the same level; however, the antioxidant activity and the content of the investigated metabolites were higher in the soil cultivation system. The callus line exhibited high differentiation in phytochemical compositions depending on the treatments and medium compositions.

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Section: Hplc-pda Analysissupporting

confidence: 52%

Section: Resultsmentioning

confidence: 99%

Section: Hplc-pda Analysismentioning

confidence: 99%

Section: Hplc-pda Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of Carlina acaulis Plant Material

et al. 2019

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Screening and semi‐quantitative determination of pentacyclic triterpenoids in plants by liquid chromatography–tandem mass spectrometry in precursor ion scan mode

Falev

Ul’yanovskii

Ovchinnikov

et al. 2020

Phytochemical Analysis

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Introduction Pentacyclic triterpenoids (PCTs) are secondary plant metabolites. They are of exceptional interest as biologically active substances and raw materials for a wide range of medications. Thus, the development of a methodology for rapid screening of PCTs in plant biomass is an important task. Objective The goal of this work was to develop an approach for simultaneous screening and semi‐quantitative determination of PCTs in plant tissues by liquid chromatography–tandem mass spectrometry with a precursor ion scan (PrecIS). Materials and methods Pressurised liquid extraction (PLE) with methanol was used for the isolation of PCTs from plant biomass. Screening and semi‐quantitative determination of PCTs in the obtained extracts were carried out by reversed phase high‐performance liquid chromatography–tandem mass spectrometry in a PrecIS mode. Results The product ion at m/z 95 with collision energy of 40 V was used as a diagnostic ion to identify PCTs by the PrecIS mode. In plant materials, 26 PCTs and their derivatives, such as PCTs esters and glycosides, were detected and identified. Calculation of the relative response factor for nine available PCTs showed that using a betulin calibration curve allows us to estimate the semi‐quantitative content of PCTs and their derivatives in plant PLE extracts. Conclusion The developed approach can be applied for simultaneous untargeted screening and semi‐quantitative determination of PCTs and their derivatives in various plants at sub‐parts per million levels.

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Bioactive compounds of Carlina acanthifolia All. roots obtained by fractional extraction and their 3D fluorescence spectra

Petkova,

Ivanov,

Saralieva

et al. 2024

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Carlina species as a new source of bioactive pentacyclic triterpenes

Cited by 39 publications

References 29 publications

The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of Carlina acaulis Plant Material

The Impact of Different Cultivation Systems on the Content of Selected Secondary Metabolites and Antioxidant Activity of Carlina acaulis Plant Material

Screening and semi‐quantitative determination of pentacyclic triterpenoids in plants by liquid chromatography–tandem mass spectrometry in precursor ion scan mode

Bioactive compounds of Carlina acanthifolia All. roots obtained by fractional extraction and their 3D fluorescence spectra

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