CARK1 phosphorylates subfamily III members of ABA receptors

Li, Xiaohua; Kong, Xiangge; Huang, Qiaorong; Zhang, Qian; Ge, Hu; Zhang, Liang; Li, Gaoming; Lü, Peng; Liu, Zhibin; Wang, Jianmei; Li, Xufeng; Yang, Yi

doi:10.1093/jxb/ery374

Cited by 31 publications

(38 citation statements)

References 37 publications

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“…Many studies have shown that ABA has important biological functions and is an important signalling molecule under abiotic stresses [ 34 – 36 ]. The expression of genes that participate in the ABA signalling pathway can increase plant abiotic tolerance [ 37 , 38 ]. In this study, compared with WT plants, transgenic Th2CysPrx tobacco exhibited increased germination rates, root lengths and chlorophyll contents under salt stress (Fig.…”

Section: Discussionmentioning

confidence: 99%

A 2-Cys peroxiredoxin gene from Tamarix hispida improved salt stress tolerance in plants

et al. 2020

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Background: Peroxiredoxins (Prxs) are a large family of antioxidant enzymes that respond to biotic and abiotic stress by decomposing reactive oxygen species (ROS). In this study, the stress tolerance function of the Th2CysPrx gene was further analysed. It lays a foundation for further studies on the salt tolerance molecular mechanism of T. hispida and improved salt tolerance via transgenic plants. Results: In this study, the stress tolerance function of the Th2CysPrx gene was further analysed. The results of transgenic tobacco showed higher seed germination rates, root lengths, and fresh weight under salt stress than wild-type tobacco. Simultaneously, physiological indicators of transgenic tobacco and T. hispida showed that Th2CysPrx improved the activities of antioxidant enzymes and enhanced ROS removal ability to decrease cellular damage under salt stress. Moreover, Th2CysPrx improved the expression levels of four antioxidant genes (ThGSTZ1, ThGPX, ThSOD and ThPOD). Conclusions: Overall, these results suggested that Th2CysPrx enhanced the salt tolerance of the transgenic plants. These findings lay a foundation for further studies on the salt tolerance molecular mechanism of T. hispida and improved salt tolerance via transgenic plants.

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Section: Discussionmentioning

confidence: 99%

A 2-Cys peroxiredoxin gene from Tamarix hispida improved salt stress tolerance in plants

et al. 2020

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“…It can change the stomata closure to regulate the transpiration rate and regulate the synthesis of osmotic substances to improve the tolerance of plants (Liu et al, 2005; Tuteja, 2007). The expression of some genes can also increase the plant tolerance by participating in the ABA signaling pathway (Li et al, 2019a; Li et al, 2019b). All evidence suggests that ABA is a key hormone in response to abiotic stress.…”

Section: Discussionmentioning

confidence: 99%

Over-Expression of ERF38 Gene Enhances Salt and Osmotic Tolerance in Transgenic Poplar

et al. 2019

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Ethylene response factor (ERF) gene family plays an important role in abiotic stress responses. In this study, we isolated a salt-inducible ERF gene, ERF38 (Potri.006G138900.1), from the 84K poplar (Populus alba × Populus glandulosa) and investigated its functions in salt and osmotic tolerance. We identified that ERF38 protein was targeted to nucleus and had no self-activation. Results from yeast-one-hybrid indicated that the ERF38 protein can specifically bind to the dehydration responsive element (DRE). We then successfully transferred the ERF38 gene into the 84K poplar. Under respective salt and polyethylene glycol (PEG)-6000 stresses, four of the physiological traits, including peroxidase (POD) and superoxide dismutase (SOD) activities, soluble protein content, and proline content, increased significantly in the transgenic plants, compared to the wild type. Regarding the other two parameters, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content, their increments in the transgenic lines under the stresses, which were compared to the water control, were significantly low than that of the wild type. In addition, reactive oxygen species (ROS) are scavenged in the transgenic lines under the stresses, but not in the wild type (WT). Interestingly, when challenged with the stresses, expression levels of a few genes associated with POD and SOD metabolism were significantly increased in the transgenic poplars. In all, evidence from morphological, physiological, and biochemical analyses indicated that over-expression of ERF38 gene can improve salt and osmotic tolerance in the transgenic poplar.

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“…The plasma membrane‐localized leucine‐rich receptor‐like kinase CEPR2 phosphorylates PYLs at a conserved site corresponding to PYL4 Ser54 and promotes degradation of PYLs (Yu et al 2019). A putative receptor‐like cytoplasmic kinase (RLCK) VIII subfamily kinase CARK1 phosphorylates PYR1 and PYL1/2/3/8 at a less conserved site corresponding to PYR1 Thr78 which enhances ABA responses, and the cark1 mutant is less sensitive to ABA (Zhang et al 2018b; Li et al 2019b). CARK1 is activated by ABA, but whether CARK1 be activated by environmental changes or other hormones is still unknown (Zhang et al 2018b).…”

Section: Core Aba Signalingmentioning

confidence: 99%

Abscisic acid dynamics, signaling, and functions in plants

Chen

Bressan

et al. 2020

JIPB

838

628

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Abscisic acid (ABA) is an important phytohormone regulating plant growth, development, and stress responses. It has an essential role in multiple physiological processes of plants, such as stomatal closure, cuticular wax accumulation, leaf senescence, bud dormancy, seed germination, osmotic regulation, and growth inhibition among many others. Abscisic acid controls downstream responses to abiotic and biotic environmental changes through both transcriptional and posttranscriptional mechanisms. During the past 20 years, ABA biosynthesis and many of its signaling pathways have been well characterized. Here we review the dynamics of ABA metabolic pools and signaling that affects many of its physiological functions.

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CARK1 phosphorylates subfamily III members of ABA receptors

Abstract: CARK1 preferentially interacts with and phosphorylates ABA receptors of subfamily III; the phosphorylation site RCAR11T78 plays a substantial role in the activation of the ABA response pathway.

Cited by 31 publications

References 37 publications

A 2-Cys peroxiredoxin gene from Tamarix hispida improved salt stress tolerance in plants

A 2-Cys peroxiredoxin gene from Tamarix hispida improved salt stress tolerance in plants

Over-Expression of ERF38 Gene Enhances Salt and Osmotic Tolerance in Transgenic Poplar

Abscisic acid dynamics, signaling, and functions in plants

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