Cargo trafficking from the trans‐Golgi network towards the endosome

Gadila, Shiva Kumar Goud; Kim, Dong Kyu

doi:10.1111/boc.201600001

Cited by 21 publications

(11 citation statements)

References 139 publications

(178 reference statements)

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“…Also, vesicles that bud from the trans-GA and are part of the constitutive secretory pathway are equipped with specific membrane proteins, and their localization depends on the same pathways described for lysosomes (Gadila and Kim, 2016). We are not aware of GEIs specifically targeted to the lumen of these organelles.…”

Section: Targeting Geis To Organellesmentioning

confidence: 99%

Exploring cells with targeted biosensors

Pendin

Greotti

Lefkimmiatis

et al. 2016

Journal of General Physiology

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Cellular signaling networks are composed of multiple pathways, often interconnected, that form complex networks with great potential for cross-talk. Signal decoding depends on the nature of the message as well as its amplitude, temporal pattern, and spatial distribution. In addition, the existence of membrane-bound organelles, which are both targets and generators of messages, add further complexity to the system. The availability of sensors that can localize to specific compartments in live cells and monitor their targets with high spatial and temporal resolution is thus crucial for a better understanding of cell pathophysiology. For this reason, over the last four decades, a variety of strategies have been developed, not only to generate novel and more sensitive probes for ions, metabolites, and enzymatic activity, but also to selectively deliver these sensors to specific intracellular compartments. In this review, we summarize the principles that have been used to target organic or protein sensors to different cellular compartments and their application to cellular signaling.

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Section: Targeting Geis To Organellesmentioning

confidence: 99%

Exploring cells with targeted biosensors

Pendin

Greotti

Lefkimmiatis

et al. 2016

Journal of General Physiology

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“…Clathrin-mediated vesicle trafficking in eukaryotes is involved in most endocytic pathways ( 1 ), trafficking from trans-Golgi network ( 2 ), trafficking from endosomes ( 3 ), and also in the critical function of regenerating synaptic vesicles (SVs) ( 4 – 6 ). Clathrin and its associated adaptor/accessory proteins together carry out three important functions, which include engaging with cargo and membrane and also generating negative curvature on the membrane such that the cargo becomes encapsulated in a vesicle.…”

Section: Introductionmentioning

confidence: 99%

The structures of natively assembled clathrin-coated vesicles

et al. 2020

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Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy chain (CHC), clathrin light chain (CLC), and a variety of adaptor protein complexes. Much is known about the structures of the individual CCV components, but data are lacking about the structures of the fully assembled complexes together with membrane and in complex with cargo. Here, we determined the structures of natively assembled CCVs in a variety of geometries. We show that the adaptor β2 appendages crosslink adjacent CHC β-propellers and that the appendage densities are enriched in CCV hexagonal faces. We resolve how adaptor protein 2 and other associated factors in hexagonal faces form an assembly hub with an extensive web of interactions between neighboring β-propellers and propose a structural model that explains how adaptor binding can direct the formation of pentagonal and hexagonal faces.

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“…A recent study by Chen and colleagues indicated that neosynthesized TfR was sorted out with the Lamp1 protein in vesicles exiting the TGN (37). These vesicles were devoid of the mannose-6P receptor (M6PR), which was used as a marker for Golgi-to-endosome route (46). In our hands, we found that M6PR was absent of the TfR-eRUSH vesicles harboring Rab7 (not shown).…”

Section: Discussionmentioning

confidence: 99%

CRISPR-based bioengineering of the Transferrin Receptor revealed a role for Rab7 in the biosynthetic secretory pathway

Deffieu

Cesonyte

Delalande

et al. 2020

Preprint

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The regulated secretory trafficking of neosynthesized transmembrane receptors is particularly challenging to investigate as it is under-represented at steady state compared to the abundance of the other trafficking routes. Here, we combined the retention using selective hook (RUSH) system to a CRISPR/Cas9 gene editing approach (eRUSH) to identify molecular players involved in the trafficking of neosynthesized Transferrin Receptor (TfR) en route to the plasma membrane (PM). TfR-eRUSH monoclonal cells expressing endogenous, ER-retainable and fluorescent TfR were engineered and characterized. Spatiotemporal quantitative proteomics of TfR-eRUSH cells allowed the identification of molecular partners associated with TfR-containing membranes and provided a comprehensive list of potential regulators, co-trafficking cargos, and enriched pathways. Furthermore, we chose to focus our attention on the Rab GTPase family members for their function as vesicle trafficking regulators and performed a Rab-targeted siRNA screen that we correlated to our proteomics data. Unexpectedly, we identified Rab7-harboring vesicles as an intermediate compartment of the Golgi-to-PM transport of the neosynthetic TfR. These vesicles did not exhibit degradative properties and were not associated to Rab6A-harboring vesicles, also involved in Golgi-to-PM transport. However, Rab6A-TfR vesicles delivered TfR directly to the PM, while in contrast, Rab7A was transiently associated to neosynthetic TfR-containing post-Golgi vesicles but dissociated before PM vesicle fusion. Together, our study proposes the eRUSH as a powerful tool to further study the secretory pathway and reveals an unforeseen role for Rab7 in the neosynthetic transport of the TfR, highlighting the diversity of the secretory vesicles’ nature for a given cargo.

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Cargo trafficking from the trans‐Golgi network towards the endosome

Cited by 21 publications

References 139 publications

Exploring cells with targeted biosensors

Exploring cells with targeted biosensors

The structures of natively assembled clathrin-coated vesicles

CRISPR-based bioengineering of the Transferrin Receptor revealed a role for Rab7 in the biosynthetic secretory pathway

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