Cardiovascular Tissue Engineering

Akter, F.; Hamid, Hamzaini Abdul

doi:10.1016/b978-0-12-805361-4.00006-0

Cited by 2 publications

(3 citation statements)

References 31 publications

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“…1,2 These processes are promoted by proper stimulation from the extracellular matrix (ECM), which provides the mechanical structure, growth factors, and recognition motif for growth and development. 3,4 Following an insult or injury, it is crucial to restore the function of damaged tissues. Since these processes play a key role in tissue regeneration, synthetic biomaterials have been engineered to emulate the native ECM.…”

Section: Introductionmentioning

confidence: 99%

“…These unique properties have made this biomaterial increasingly popular as a scaffold for tissue and cell engineering. 4,[20][21][22] This study introduces a new bioactive laminin peptide and ELP construct, A99 (AGTFALRGDNPQG) conjugated to I30 (VPGVG) 30 by both molecular cloning and chemical conjugation. This study demonstrates the biological activity and usefulness of laminin peptide-ELPs as a biomaterial.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of extracellular matrix mimetic laminin bioactive peptide and elastin‐like polypeptide

et al. 2020

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Abbreviations: A99, laminin α1 chain RGD-containing peptide (AGTFALRGDNPQG); A99-ELP-C, chemically conjugated CGG-A99 containing peptide to I30; A99-ELP-R, recombinant A99 fused to I30; ECM, extracellular matrix; ELP, elastin-like polypeptide; FOV, field of view; HDF, human dermal fibroblast; I30, elastin-like polypeptide with a sequence of (VPGIG) 30 ; LCST, lower critical solution temperature; MW, molecular weight; OD, optical density; RT, room temperature; T t , transition temperature. AbstractThe extracellular matrix (ECM) is comprised of a large network of proteins that are essential for tissue development and repair. A bioactive RGD-containing peptide from laminin α1 chain, A99 (AGTFALRGDNPQG), promotes strong cell attachment and has demonstrated utility in cell culture and tissue engineering. Various materials can be utilized as a scaffold for bioactive peptides; however, it may be advantageous to design materials that use bioconjugation strategies that do not affect bioactivity, generate homogenous products, and can be produced at scale. This report is the first to compare the methods for preparing chemically conjugated and recombinant A99 to elastin-like polypeptides (ELPs) as the scaffold and characterize the biological and cell attachment activity using human dermal fibroblasts (HDFs). ELPs are biocompatible protein-polymers that are also thermo-responsive. Below a lower critical solution temperature (LCST), they are highly soluble. Above the LCST, ELPs phase separate into a polymer-rich liquid, known as a coacervate. Both chemically conjugated and recombinant fusion between A99 and an ELP (A99-ELP-R) show dose-dependent cell attachment. In addition, coating above the LCST provides better cell spreading compared to coating at 4°C. ELPs provide an excellent structural framework for deposition of bioactive peptides of the ECM, and their intrinsic biophysical properties make laminin peptide-ELPs promising biomaterials for cell culture and tissue engineering. K E Y W O R D S biomaterial, elastin-like polypeptide, laminin peptide, synthetic biology, tissue engineering 6730 | TRUONG eT al.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Evaluation of extracellular matrix mimetic laminin bioactive peptide and elastin‐like polypeptide

et al. 2020

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“…Proper vascularization is essential in organogenesis and tissue regeneration as it ensures adequate transport of nutrients, metabolites, and oxygen to support cell viability and tissue development. [1,2] A functional vasculature is particularly DOI: 10.1002/mabi.202300213 important in regenerating volumetric or metabolically active tissues as it prevents hypoxia and necrosis. [3] A variety of approaches have been developed to fabricate vascularized 3D constructs, including endothelial cells-laden bioactive and degradable hydrogels, [4,5] porous polymeric scaffolds, [6,7] and matrices/scaffolds with perfusable channels.…”

Section: Introductionmentioning

confidence: 99%

Digital Light Processing 3D Bioprinting of Gelatin‐Norbornene Hydrogel for Enhanced Vascularization

Duong

Lin

2023

Macromolecular Bioscience

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Digital light processing (DLP) bioprinting can be used to fabricate volumetric scaffolds with intricate internal structures, such as perfusable vascular channels. The successful implementation of DLP bioprinting in tissue fabrication requires using suitable photo‐reactive bioinks. Norbornene‐based bioinks have emerged as an attractive alternative to (meth)acrylated macromers in 3D bioprinting owing to their mild and rapid reaction kinetics, high cytocompatibility for in situ cell encapsulation, and adaptability for post‐printing modification or conjugation of bioactive motifs. In this contribution, we report the development of gelatin‐norbornene (GelNB) as a photocrosslinkable bioink for DLP 3D bioprinting. Low concentrations of GelNB (2‐5 wt%) and poly(ethylene glycol)‐tetra‐thiol (PEG4SH) were DLP‐printed with a wide range of stiffness (G' ∼120 to 4,000 Pa) and with perfusable channels. DLP‐printed GelNB hydrogels were highly cytocompatible, as demonstrated by the high viability of the encapsulated human umbilical vein endothelial cells (HUVECs). The encapsulated HUVECs formed an interconnected microvascular network with lumen structures. Notably, the GelNB bioink permitted both in situ tethering and secondary conjugation of QK peptide, a vascular endothelial growth factor (VEGF)‐mimetic peptide. Incorporation of QK peptide significantly improved endothelialization and vasculogenesis of the DLP‐printed GelNB hydrogels, reinforcing the applicability of this bioink system in diverse biofabrication applications.This article is protected by copyright. All rights reserved

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Cardiovascular Tissue Engineering

Cited by 2 publications

References 31 publications

Evaluation of extracellular matrix mimetic laminin bioactive peptide and elastin‐like polypeptide

Evaluation of extracellular matrix mimetic laminin bioactive peptide and elastin‐like polypeptide

Digital Light Processing 3D Bioprinting of Gelatin‐Norbornene Hydrogel for Enhanced Vascularization

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