Cardiovascular protective effect of polysaccharide from Ophiopogon japonicus in diabetic rats

Zhang, Junfeng; Fan, Sairong; Mao, Yihua; Ji, Yuan; Jin, Liqin; Lü, Jianxin; Chen, Xiaoming

doi:10.1016/j.ijbiomac.2015.09.069

Cited by 50 publications

(21 citation statements)

References 31 publications

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“…Western medicine has stated that coronary heart disease is a chronic inflammatory process (Yin deficiency with internal heat), lipid accumulation (sputum) and migration and proliferation of smooth muscle cells (blood stasis). Luckily, Granule of BU-XIN RUAN-MAI is just the compound prescription that it may nourish Qi and Yin to enhance blood circulation for clearing internal heat and removing obstruction in collaterals, and products of these herbs exhibit perfect activity of anti-inflammation, antioxidative stress, antithrombotic activity, and cardiovascular protective activity [20][21][22][23][24][25][26][27][28][29][30][31][51][52][53][54][55][56]. Consequently, Granule of BU-XIN RUAN-MAI is a perfect prescription for treatment of ischemic heart disease (angina pectoris).…”

Section: Discussionmentioning

confidence: 99%

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Dong

Zhao

Yue

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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Objective. Coronary heart disease (CHD) has been regarded as a serious and common disease in the modern society. This study aims to investigate the effect of Granule of BU-XIN RUAN-MAI (BXRM) on angina pectoris of coronary heart disease and to explore the molecular mechanisms underlying Granule of BU-XIN RUAN-MAI-mediated protective activity against this disease. Methods. The effects of Granule of BU-XIN RUAN-MAI on clinical symptoms of patients’ angina were indicated by hemorheology indicators including high shear of blood viscosity, low shear of blood viscosity, plasma viscosity, erythrocyte rigidity index, D-D dimer, fibrinogen content, and lipid content. The effects of Granule of BU-XIN RUAN-MAI on isoprenaline-induced myocardial cell injury were determined by conducting H&E staining and by performing ELISA to examine the serum content of MDA, SOD, Na+/K+-ATPase, cAMP, and the content of inflammatory factors in isoprenaline-induced rats. Meanwhile, western blot and real time PCR were used to determine the expression of genes involved in oxidation and energy metabolism, and real time PCR was also used for determination of miR-542-3p expression. Luciferase reporter assay was conducted to test the binding sites of miR-542-3p on GABARAP 3′UTR. The chemical compositions of Granule of BU-XIN RUAN-MAI were determined by liquid LC-QTOF-MS. Results. Granule of BU-XIN RUAN-MAI significantly attenuated the clinical symptoms of patients’ angina by improving the patients’ heart rate and by decreasing the level of hemorheology indicators and also by reducing the serum content of TC, TG, LDL, and elevated HDL content. H&E staining demonstrated that Granule of BU-XIN RUAN-MAI ameliorated the myocardial ischemia in a dose-dependent manner. Besides, Granule of BU-XIN RUAN-MAI downregulated serum MDA content and upregulated the content of SOD, Na+/K+-ATPase, and cAMP in isoprenaline-induced rats. Granule of BU-XIN RUAN-MAI significantly improved oxidation stress by increasing PPARα expression, and it inhibited inflammation by downregulating expression and contents of IL-6, IL-1β, and TNF-α. Then, Granule of BU-XIN RUAN-MAI-containing serum increased the SOD content, and reduced the MDA content in angiotensin II-stimulated HUVEC cells. The granule of BU-XIN RUAN-MAI-containing serum obviously downregulated protein expressions of P40phox, P47phox, and P67phox in plasma membrane, and it significantly increased protein levels of P40phox, P47phox, and P67phox in the cytoplasm of HUVEC cells. Furthermore, GABARAP was reduced in heart tissues of ISO-induced rats and in angiotensin II-stimulated cell lines, and GABARAP was required for the inhibitory activity of Granule of BU-XIN RUAN-MAI on oxidation and inflammation in vivo and in vivo. GABARAP could be upregulated by Granule of BU-XIN RUAN-MAI by inhibiting the expression of miR-542-3p, which may significantly enhance oxidation and inflammation by targeting GABARAP in cardiomyocytes. Moreover, the silencing of GABARAP could obviously reverse the granule of BU-XIN RUAN-MAI-mediated protective activity against coronary heart disease, and interfering GABARAP expression also could partly block the anti-miR-542-3p-controlled oxidation and inflammation in cardiomyocytes. Besides, salidroside, loganin, and polydatin were the main compounds of granules of BU-XIN RUAN-MAI. Conclusion. Granule of BU-XIN RUAN-MAI is an excellent prescription for treatment of coronary heart disease by suppressing inflammation and NAPDH-mediated oxidative stress. The miR-542-3p/GABARAP axis is required for Granule of BU-XIN RUAN-MAI, exhibiting its protective activity against the pectoris of coronary heart disease.

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Section: Discussionmentioning

confidence: 99%

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Dong

Zhao

Yue

et al. 2019

Evidence-Based Complementary and Alternative Medicine

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“…Plant polysaccharides have biological effects such as immunoregulation, anticancer, antiaging, serum glucose-depressing, and lipid-depressing [1][2][3][4][5]. And recently a growing number of studies have demonstrated the significant advantages of polysaccharides in the prevention and treatment of critical or chronic diseases in human such as cancers, cardiovascular diseases, and diabetes [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Anticancer Effects of Cyclocarya paliurus Polysaccharide (CPP) on Thyroid Carcinoma In Vitro and In Vivo

Gan

et al. 2018

International Journal of Polymer Science

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Correspondence should be addressed to Ting Que; tque@outlook.com Zhiwei He and Fangfang Lv contributed equally to this work.In this study, we explored the role and mechanisms of Cyclocarya paliurus polysaccharide on cell apoptosis in thyroid cancer (TC) cells. The apoptosis of thyroid cancer cells in vitro and tumor tissues in vivo induced by Cyclocarya paliurus polysaccharide was determined by MTT assay and flow cytometric assay. The downstream molecules including phosphop-protein kinase B (p-Akt), Akt, B-cell lymphoma 2 (Bcl-2), and Bcl-2-associated X protein (Bax) in tumor tissue were evaluated by western blotting. MTT and flow cytometry assay in vitro revealed Cyclocarya paliurus polysaccharide-induced apoptosis of thyroid cancer cell line in a manner of time-dependent and dose-dependent. In vivo assay showed 50 mg/kg and 100 mg/kg Cyclocarya paliurus polysaccharide significantly suppressed the proliferation of thyroid cancer in mice. Western blotting showed downregulation of p-Akt, Akt, and Bcl-2 and upregulation of Bax. These results suggest that Cyclocarya paliurus polysaccharide may enhance thyroid cancer cell apoptosis by suppressing the activation of p-Akt, Akt, and Bcl-2 and activating Bax, which provide a novel use of CPP as a thyroid cancer treatment.

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“…The main chemical constituents were isoflavones, volatile oils, saponins, inorganic elements, alcohols and polysaccharides [7] [8]. The polysaccharide was one of the main active components in Ophiopogon japonicus, which has the functions of anti-fatigue, hypoglycemic activity, anti-radiation, auxiliary inhibition of tumor and so on [9] [10] [11]. In this research, ultrasound with different power was employed to extract polysaccharides from Ophiopogon japonicus.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity <i>in Vitro</i> of Polysaccharide Extracted by Ultrasound with Different Powers from <i>Ophiopogon japonicas</i>

Wang¹,

Zhang²,

Zhang³

et al. 2018

AJPS

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The polysaccharides were extracted by different power ultrasound from Ophiopogon japonicus. The extraction yield and in vitro antioxidant activities including scavenging effect on hydroxyl radical, superoxide anion free radicals and DPPH were investigated. The results showed that with the increase of ultrasonic power, the yield of the polysaccharides decreased first and then increased. The antioxidant activity of polysaccharides increased first and then decreased with the increase of ultrasonic power. When the power was 400 W, the scavenging effect on superoxide anion and DPPH of the polysaccharides was the strongest, and the scavenging ability of hydroxyl radical was the strongest when the power was 560 W. Therefore, different ultrasonic power could affect the extraction yield and antioxidant activity of Ophiopogon japonicus polysaccharide. It will provide theoretical basis and experimental support for the application of ultrasonic extraction on polysaccharides from Ophiopogon japonicus.

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Cardiovascular protective effect of polysaccharide from Ophiopogon japonicus in diabetic rats

Cited by 50 publications

References 31 publications

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Anticancer Effects of Cyclocarya paliurus Polysaccharide (CPP) on Thyroid Carcinoma In Vitro and In Vivo

Antioxidant Activity <i>in Vitro</i> of Polysaccharide Extracted by Ultrasound with Different Powers from <i>Ophiopogon japonicas</i>

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Cardiovascular protective effect of polysaccharide from Ophiopogon japonicus in diabetic rats

Cited by 50 publications

References 31 publications

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Anticancer Effects of Cyclocarya paliurus Polysaccharide (CPP) on Thyroid Carcinoma In Vitro and In Vivo

Antioxidant Activity &lt;i&gt;in Vitro&lt;/i&gt; of Polysaccharide Extracted by Ultrasound with Different Powers from &lt;i&gt;Ophiopogon japonicas&lt;/i&gt;

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Antioxidant Activity <i>in Vitro</i> of Polysaccharide Extracted by Ultrasound with Different Powers from <i>Ophiopogon japonicas</i>