Cardiovascular Lesions and Changes in Blood Coagulation and Fibrinolysis Associated With Diet-Induced Lipemia in the Rat*

Naimi, Shapur; Goldstein, Robert H.; Nothman, Martin M.; Wilgram, George F.; Proger, Samuel

doi:10.1172/jci104628

Cited by 19 publications

(4 citation statements)

References 12 publications

(7 reference statements)

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“…A number of studies suggest that diet affects blood coagulation in both acute (6,7) and longerterm studies (1). There is evidence from animal studies (8)(9)(10)(11) and in man from epidemiological sources (12)(13)(14) that dietary fat influences thrombosis and the complications of vascular disease. So far there have been no quantitative reports published concerning the effects of dietary manipulations on thrombus formation in flowing blood in the arterial circulation.…”

mentioning

confidence: 99%

Diet and Thrombus Formation: Quantitative Studies Using an Extracorporeal Circulation in Pigs*

Mustard¹,

Rowsell²,

Murphy³

et al. 1963

J. Clin. Invest.

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Although there has been widespread interest in the relationship of diet to atherosclerosis and its complications, much less attention has been paid to the effect of diet on thrombus formation. For the most part, exploration of this problem has been indirect; in the first place, it has been supposed that in vitro tests of coagulation are good measures of the coagulant activity of the blood and thrombus formation; and in the second, it has been supposed that coagulant activity in vivo reflects tendency to form thrombi. In a previous study (1), we attempted to circumvent the former supposition by directing our attention to platelet survival, which can be studied in vivo in man and in experimental animals. The ideal method of obviating the latter supposition would be to devise methods of quantitating thrombus formation in the intact animal. Semiquantitative grading systems have been used by some investigators. As yet, however, no satisfactory, precise quantitative method has been devised.Connor and Poole (2) and Connor (3), recognizing that movement of the blood, particularly in the arterial circulation, may be an important factor in thrombus formation, used Chandler's apparatus (4) to study the effect of various fatty acids on thrombus formation. In their experiments, citrated blood was mixed with the fatty acid for one minute. It was then recalcified inside the apparatus, and the time for the appearance of clotting was noted.We have described elsewhere a method for quan-*

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mentioning

confidence: 99%

Diet and Thrombus Formation: Quantitative Studies Using an Extracorporeal Circulation in Pigs*

Mustard¹,

Rowsell²,

Murphy³

et al. 1963

J. Clin. Invest.

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“…Among the animal models which hyperlipidemia has been experimentally induced, the rat has been the object of particular interest because of its omnivorous dietary habits (Wissler et al, 1954;Naimi et al, 1962). Data show that exposure of animals to a high fat diet for 120 days leads to development of hyperlipidemia and/or atherosclerosis as evidenced by significant increases in lipid profiles.…”

Section: Resultsmentioning

confidence: 99%

Isolation of phytosterols and antihyperlipidemic activity of Lagenaria siceraria

Kalsait¹,

Khedekar

Saoji

et al. 2011

Arch. Pharm. Res.

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Lagenaria siceraria (Molina) Standl in the Cucurbitaceae family is a large, pubescent, climbing, or trailing herb cultivated throughout India and the tropical regions of the world. Phytochemical investigation of the methanol extract obtained from the fruits of the plant L. siceraria showed a positive Liebermann-Burchard test for sterols. The white sterol crystals or phytosterols from the methanol extract were isolated for the first time and identified as a mixture of four sterols, including fucosterol (1), racemosol (2), stigmasterol (3), and stigmasta-7,22-dien-3β,4β-diol (4). These compounds were identified by spectroscopic evidence including FTIR, (1)H-NMR, MS, and GC. The white sterol crystals, which are the mixture of four sterols, were evaluated for antihyperlipidemic activity in Wistar rats. The blood samples were collected from the retro-orbital plexus and serum was separated and analyzed for lipid profiles. These sterol crystals (30 mg/kg) showed significant reductions in lipid profiles which included cholesterol, triglycerides, LDL and VLDL. In addition, a significant increase in HDL cholesterol observed, which is a good cholesterol that protects hearts from coronary artery diseases. These sterol crystals or phytosterols can be used as an antihyperlipidemic agent to treat the hyperlipidemic.

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“…By the addition of saturated fat to this type of diet, Thomas and Hartroft (4) were able to show that myocardial infarction could be produced even if the vitamin D was omitted. A number of other authors have published data on blood coagulation in similar animals (5,6,7,8); from these studies it is fairly certain that a marked elevation of many coagulation factors was present in these rats. It has been postulated that a "hypercoagulable" state is an important factor in the genesis of the infarcts (9) .…”

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confidence: 90%

Changes in Blood Coagulation and Fibrinolysis in Rats Fed Atherogenic Diets

Merskey¹,

Wohl²

1963

Thromb Haemost

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Summary1. Groups of rats were fed thrombogenic diets and the effects on blood coagulation and fibrinolysis assessed.2. Animals fed a diet containing cholesterol, thiouracil and cholic acid developed high levels of coagulation factors I, II, V, VII—X, VIII, IX and X.3. Animals fed a similar diet with additional 40% beef fat developed even greater elevation of V, VII—X, VIII and X, similar elevation of factor II, and lesser (but still significant) elevation of factors I and IX. In addition marked elevation of blood platelets occurred.4. Euglobulin lysis time of the group not fed the additional fat was longer than in controls. Significant prolongation of euglobulin lysis time was not found in the group fed additional fat.5. If the increased levels of plasma fibrinogen were taken into account, it was found that a larger amount of fibrin was lysed per unit time in the euglobulin lysis test with plasma from rats fed either atherogenic diet compared with controls.6. Defective thromboplastin generation was present in both groups of rats fed an atherogenic diet. The defect was present in the serum and was not due to lack of a factor required for thromboplastin generation. An inhibitor was present in the serum which was capable of preventing the action of normal serum.7. No good correlation was found between the occurrence of changes in blood coagulation or fibrinolysis and the presence or absence of thrombosis and infarction.8. The exact cause of these anomalies remains unexplained, as does the cause of the thrombosis in these animals. Starvation per se does not account for these abnormal findings. They could not adequately be explained on the basis of “hypercoagulability” of the blood.

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Cardiovascular Lesions and Changes in Blood Coagulation and Fibrinolysis Associated With Diet-Induced Lipemia in the Rat*

Cited by 19 publications

References 12 publications

Diet and Thrombus Formation: Quantitative Studies Using an Extracorporeal Circulation in Pigs*

Diet and Thrombus Formation: Quantitative Studies Using an Extracorporeal Circulation in Pigs*

Isolation of phytosterols and antihyperlipidemic activity of Lagenaria siceraria

Changes in Blood Coagulation and Fibrinolysis in Rats Fed Atherogenic Diets

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