Cardiovascular and growth outcomes of C57Bl/6J mice offspring exposed to maternal stress and ionizing radiation during pregnancy

Sreetharan, Shayenthiran; Stoa, Lisa; Cybulski, Mary Ellen; Jones, Devon; Lee, Abigail H.; Kulesza, Adomas; Tharmalingam, Sujeenthar; Boreham, Douglas R.; Tai, T.C.; Wilson, Joanna Y.

doi:10.1080/09553002.2019.1589025

Cited by 8 publications

(6 citation statements)

References 37 publications

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“…In males, the 1000 mGy group had reduced heart weight, but this was accompanied by lower body weight. We have previously reported a reduction in body weight in the 1000 mGy group [44]. Previous studies have reported a correlation between low birth weight or restricted growth in offspring and an increased incidence of cardiovascular disease in adulthood.…”

Section: Discussionmentioning

confidence: 64%

“…All animals were restricted for food and water consumption for the duration of irradiation. The fetal dose was verified by using the thermoluminescent dosimeters as previously described by our group [44]. A maximum of 2 male and 2 female offspring from one dam was used in this study to control for any maternal biased effects.…”

Section: Animal Care and Experimental Groupsmentioning

confidence: 99%

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Lasting Effects of Low to Non-Lethal Radiation Exposure during Late Gestation on Offspring’s Cardiac Metabolism and Oxidative Stress

et al. 2021

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Ionizing radiation (IR) is known to cause fetal programming, but the physiological effects of low-dose IR are not fully understood. This study examined the effect of low (50 mGy) to non-lethal (300 and 1000 mGy) radiation exposure during late gestation on cardiac metabolism and oxidative stress in adult offspring. Pregnant C57BL/6J mice were exposed to 50, 300, or 1000 mGy of gamma radiation or Sham irradiation on gestational day 15. Sixteen weeks after birth, 18F-Fluorodeoxyglucose (FDG) uptake was examined in the offspring using Positron Emission Tomography imaging. Western blot was used to determine changes in oxidative stress, antioxidants, and insulin signaling related proteins. Male and female offspring from irradiated dams had lower body weights when compared to the Sham. 1000 mGy female offspring demonstrated a significant increase in 18F-FDG uptake, glycogen content, and oxidative stress. 300 and 1000 mGy female mice exhibited increased superoxide dismutase activity, decreased glutathione peroxidase activity, and decreased reduced/oxidized glutathione ratio. We conclude that non-lethal radiation during late gestation can alter glucose uptake and increase oxidative stress in female offspring. These data provide evidence that low doses of IR during the third trimester are not harmful but higher, non-lethal doses can alter cardiac metabolism later in life and sex may have a role in fetal programming.

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Section: Discussionmentioning

confidence: 64%

Section: Animal Care and Experimental Groupsmentioning

confidence: 99%

Lasting Effects of Low to Non-Lethal Radiation Exposure during Late Gestation on Offspring’s Cardiac Metabolism and Oxidative Stress

et al. 2021

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“…In rats, also cold stress during the last week of pregnancy was associated with an increase in SBP (Sun et al, 2019). Finally, one study conducted in C57BL/6 J mice reported a detrimental but sex-specific effect on SBP (Sreetharan et al, 2019).…”

Section: Effects Of Maternal Stress On Systolic Blood Pressurementioning

confidence: 89%

Prenatal stress and later metabolic consequences: Systematic review and meta-analysis in rodents

Burgueño

Juárez

Genaro

et al. 2020

Psychoneuroendocrinology

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Background: Numerous rodent studies have evaluated the effects of maternal stress (MS) on later in life susceptibility to Metabolic Syndrome (MetS) intermediate phenotypes with varying results. The aim of this study was to quantitatively synthesize the available data on the effects of MS on offspring obesity, estimated indirectly by body mass (BM), body fat (BF) and plasma leptin; systolic blood pressure (SBP); plasma glucose (and insulin) and blood lipid concentrations. Methods: Literature was screened and summary estimates of the effect of MS outcomes were calculated by using random-effects models. Data on the effects of exogenous corticosteroid administration (or inhibition of 11β-HSD2) during pregnancy in rodents was analysed separately to characterize the direct phenotypic effects of prenatal corticosteroid excess (PCE). Results: We conducted 14 separate meta-analyses and synthesized relevant data on outcomes scarcely reported in literature. Both MS and PCE were associated with low birth weight without rapid catch-up growth resulting in decreased body mass later in life. Our analysis also revealed significant and contradictory effects on offspring adiposity. Little evidence was found for effects on glucose metabolism and blood lipids. We identified increased SBP in offspring exposed to PCE; however, there is not enough data to draw any conclusion about effects of MS on SBP. Conclusions: Neonatal weight proved to be decreased in offspring prenatally exposed to stress or corticosteroids, but laboratory rodents in the absence of a challenging environment did not show catch-up growth. The available evidence is inconclusive regarding the effect on adiposity revealing clear methodological and knowledge gaps. This meta-analysis also confirmed a significant positive association between PCE and SBP. Nevertheless, additional studies should address the association with MS.

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“…Both thickening and enlargement can result in heart problems and even heart failure (Yusuf et al 2011). On the contrary, Sreetharan et al (2019) showed that acute doses of 1 Gy caused reduction in the heart rate of male rat offspring when mother was irradiated during pregnancy. However, again the doses given in radiation therapy and laboratory studies are much larger than those estimated for bank voles from Chernobyl (Chesser et al 2000).…”

Section: Discussionmentioning

confidence: 99%

The effect of chronic low-dose environmental radiation on organ mass of bank voles in the Chernobyl exclusion zone

Kivisaari

Boratyński

Lavrinienko

et al. 2020

International Journal of Radiation Biology

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Purpose: Animals are exposed to environmental ionizing radiation (IR) externally through proximity to contaminated soil and internally through ingestion and inhalation of radionuclides. Internal organs can respond to radioactive contamination through physiological stress. Chronic stress can compromise the size of physiologically active organs, but studies on wild mammal populations are scarce. The effects of environmental IR contamination on organ masses were studied by using a wild rodent inhabiting the Chernobyl exclusion zone (CEZ). Material and methods: The masses of brain, heart, kidney, spleen, liver and lung were assessed from bank voles (Myodes glareolus) captured from areas across radioactive contamination gradient within the CEZ. Relative organ masses were used to correct for the body mass of an individual. Results: Results showed a significant negative correlation between IR level in the environment and relative brain and kidney mass. A significant positive correlation between IR and relative heart mass was also found. Principal component analysis (PCA) also suggested positive relationship between IR and relative spleen mass; however, this relationship was not significant when spleen was analyzed separately. There was no apparent relationship between IR and relative liver or lung mass. Conclusions: Results suggest that in the wild populations even low but chronic doses of IR can lead to changes in relative organ mass. The novelty of these result is showing that exposure to low doses can affect the organ masses in similar fashion as previously shown on high, acute, radiation doses. These data support the hypothesis that wildlife might be more sensitive to IR than animals used in laboratory studies. However, more research is needed to rule out the other indirect effects such as radiosensitivity of the food sources or possible combined stress effects from e.g. infections.

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Cardiovascular and growth outcomes of C57Bl/6J mice offspring exposed to maternal stress and ionizing radiation during pregnancy

Cited by 8 publications

References 37 publications

Lasting Effects of Low to Non-Lethal Radiation Exposure during Late Gestation on Offspring’s Cardiac Metabolism and Oxidative Stress

Lasting Effects of Low to Non-Lethal Radiation Exposure during Late Gestation on Offspring’s Cardiac Metabolism and Oxidative Stress

Prenatal stress and later metabolic consequences: Systematic review and meta-analysis in rodents

The effect of chronic low-dose environmental radiation on organ mass of bank voles in the Chernobyl exclusion zone

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