Cardioprotective molecules are enriched in beating cardiomyocytes derived from human embryonic stem cells

Chae, Jung Il; Kim, Jumi; Son, Mi‐Young; Jeon, Young Joo; Kim, Dong Wook; Kim, Hye-Eun; Lee, Min Ji; Ryu, Joohyun; Park, Byoung Chul; Lee, Dong Seok; Seo, Kang Seok; Lee, Hak Kyo; Choi, Na-Young; Kang, Yong‐Kook; Chung, Hyung Min

doi:10.1016/j.ijcard.2012.07.013

Cited by 9 publications

(6 citation statements)

References 57 publications

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“…The combination of elevated CRP and TNF-α in these non-obese young women coalesces sufficiently that PCOS may link to the development of T2DM and cardiovascular disorder. On the other hand, considerable evidence demonstrates that Hsp70 attenuates inflammation via a direct response to inhibit inflammatory mediators or indirectly by suppressing downstream gene transcription of cytokines [43], suggesting a cytoprotective and an anti-autotoxicity roles for intracellular Hsp70 [44,45]. Our findings clearly clarified that serum Hsp70 was positively correlated with high levels of CRP and TNF-α in patients with PCOS.…”

Section: Discussionsupporting

confidence: 56%

Serum Heat Shock Protein 70 Concentration in Relation to Polycystic Ovary Syndrome in a Non-Obese Chinese Population

Gao

Meng

et al. 2013

PLoS ONE

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BackgroundPolycystic ovary syndrome (PCOS) represents the most common cause of anovulatory infertility and affects 6-15% of women of reproductive age. However, the underlying etiology is still poorly understood. In this study, we attempted to examine the association between circulating heat shock protein 70 (Hsp70) concentrations and PCOS in a non-obese Chinese population.Methods and ResultsHuman peripheral blood from 52 patients with PCOS and 57 healthy controls, matched for age and BMI, were analyzed. Women with PCOS were found to have significantly higher fasting insulin (FI) levels, as well as Insulin resistance index (HOMA-IR) (P < 0.05). Identically, markers of oxidative stress (malondialdehyde (MDA), 8-Hydroxy-desoxyguanosine (8-OHdG), Nitric oxide (NO)) and inflammation (tumor necrosis factor-alpha (TNF-α), C-reactive protein (CRP)) were markedly increased when compared to controls (P < 0.05). Elevated serum Hsp70 was positively correlated with IR, oxidative stress and inflammation in PCOS, even after adjustment for age, BMI and gynecologic inflammation (GI). The receiver-operating characteristic curve (ROC) analysis yielded notably different discriminative value for PCOS, with or without an addition of Hsp70 (areas under the curves were 0.884 (95% CI 0.822-0.946) vs. 0.822 (95% CI 0.744-0.900); P for difference = 0.015).Conclusions and SignificanceIncreased serum Hsp70 levels are associated with the combination of IR, oxidative stress and low-grade chronic inflammation in PCOS individuals, which provides supportive evidence that Hsp70 plays a key role in the pathogenesis of PCOS. More consequent studies were warranted to confirm the clinical utility of circulating Hsp70, especially in diagnosis and prognosis of PCOS and its long-term health cost.

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Section: Discussionsupporting

confidence: 56%

Serum Heat Shock Protein 70 Concentration in Relation to Polycystic Ovary Syndrome in a Non-Obese Chinese Population

Gao

Meng

et al. 2013

PLoS ONE

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“…An H9 human embryonic stem cell (hESC) line was obtained from the WiCell Research Institute, in Madison, WI. Somatic cells and human pluripotent stem cells (hPSCs), including hESCs and human iPSCs, were cultured as described previously . The fibroblasts were reprogrammed by electroporation with Episomal iPSC Reprogramming Vectors (Cat.…”

Section: Methodsmentioning

confidence: 99%

Distinctive genomic signature of neural and intestinal organoids from familial Parkinson's disease patient‐derived induced pluripotent stem cells

Son

Sim

Son

et al. 2017

Neuropathology Appl Neurobio

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“…Synthetically, beating cardiac-committed cells may play an important role in cardiac remodeling when transplanted in an ischemic heart because of their upregulated cardioprotection protein expression profile and the upregulation of antioxidant and cardiac remolding molecules 8 . To the best of our knowledge, the majority of stem cell-derived CMs with spontaneous beating capacity originated from embryonic stem cells (ESCs) 8 9 10 11 , induced pluripotent stem cells (iPSCs) 11 12 , cardiac progenitor cells (CPCs) 13 14 15 , and stromal vascular fraction (SVF) 16 17 18 . However, the sources of ESCs, iPSCs, and CPCs are confronted with controversy or have poor accessibility.…”

mentioning

confidence: 99%

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Yang

Xiao

Ren

et al. 2017

Sci Rep

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Heart failure often develops after acute myocardial infarction because the injured myocardial tissue fails to recover or regenerate. Stem cell transplantation using adult cell sources, such as adipose-derived stromal vascular fraction (SVF), draws extensive attention. In this study, SVF cells were isolated from rat adipose tissue and cultivated on enzyme-crosslinked gelatin hydrogels. Morphological features of cell development and spontaneous beating behavior from these cells were observed and recorded. Cardiac phenotypes were characterized via immunofluorescence staining, and the expression of cardiac-specific genes was measured via RT-PCR. The functional assessment of SVF-derived cardiomyocyte-like cells (SVF-CMs) was performed by detecting cellular calcium transient activities and pharmacological responses. Results showed that most SVF-CMs exhibited elongated myotubule shapes and expressed cardiac troponin I strongly. SVF-CMs expressed cardiac-specific RNA (including transcription factors GATA binding protein 4) and myocyte enhancer factor 2c, as well as the structural proteins, namely, sarcomere actinin alpha 2, cardiac troponin I type 3, cardiac troponin T type 2, and cardiac gap junction protein alpha 1. Their beating mode, calcium activities, and pharmacological responses were similar to those of native CMs. Spontaneously beating SVF-CMs can be derived from adipose tissue-derived SVFs, and enzyme-crosslinked gelatin hydrogel promoted the cardiac differentiation of SVF cells.

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Cardioprotective molecules are enriched in beating cardiomyocytes derived from human embryonic stem cells

Cited by 9 publications

References 57 publications

Serum Heat Shock Protein 70 Concentration in Relation to Polycystic Ovary Syndrome in a Non-Obese Chinese Population

Serum Heat Shock Protein 70 Concentration in Relation to Polycystic Ovary Syndrome in a Non-Obese Chinese Population

Distinctive genomic signature of neural and intestinal organoids from familial Parkinson's disease patient‐derived induced pluripotent stem cells

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

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