Cardioprotective Effects of Tannic Acid on Isoproterenol-Induced Myocardial Injury in Rats: Further Insight into ‘French Paradox’

Abstract

Tannic acid (TA) is a polyphenolic compound, which has shown diverse pharmacological effects with antimutagenic, anticarcinogenic and antibactericidal properties. However, cardioprotective effects of TA have not been reported. To investigate the protective effects of TA, rats were administered TA for 7 days and then intoxicated with isoproterenol (ISO). Myocardial ischemia injury was indicated by changes in electrocardiographic (ECG) patterns, morphology and cardiac marker enzymes. Furthermore, protein expression levels of c-fos, c-jun, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), cleaved-caspase-3 and -9 were analyzed by immunohistochemistry, and activities of apoptosis-related proteins Bax, Bcl-2, caspase-3 and nuclear factor kappa B (NF-κB) were detected by Western blot. Pretreatment with TA ameliorated changes in morphology and ECG, reduced activities of marker enzymes, suppressed overexpression of apoptosis-related proteins, upregulated expression of antioxidants. Moreover, TA pretreatment contributed to the decrease in ratio of Bax/Bcl-2, as well as reduced expression of TNF-α, IL-1β, caspase-3, cleaved-caspase-3 and -9. TA displayed cardioprotective effects, which may be attributed to lowering of Bax/Bcl-2 ratio, c-fos and c-jun expression and inhibition of NF-κB activation, as well as oxidative stress, inflammation and apoptosis. These findings provide further insight into the 'French paradox' and the mechanisms underlying the beneficial effects of TA. Copyright © 2015 John Wiley & Sons, Ltd.

Effects of dietary level of tannic acid and protein on internal organ weights and biochemical blood parameters of rats

“…The biochemical blood profile was only slightly affected by TA level. Feeding diets with 2% of TA considerably reduced the activity of CK, a cardiac marker enzyme, which was in agreement with previous findings of cardioprotective effects of TA and other polyphenols [ 4 , 26 , 27 ]. It has recently been suggested that TA maintains the integrity of the cardiac cell membrane and prevents the leakage of CK and other cytosolic enzymes into the bloodstream [ 4 ], which seems to be confirmed by the results of our study.…”

“…Feeding diets with 2% of TA considerably reduced the activity of CK, a cardiac marker enzyme, which was in agreement with previous findings of cardioprotective effects of TA and other polyphenols [ 4 , 26 , 27 ]. It has recently been suggested that TA maintains the integrity of the cardiac cell membrane and prevents the leakage of CK and other cytosolic enzymes into the bloodstream [ 4 ], which seems to be confirmed by the results of our study. TA may exert its cardioprotective effect by reduction of Bax/Bcl-2 ratio, expression of c-fos and c-jun, inhibition of NF-κB activation, as well as due to antioxidative and antiinflammatory properties [ 4 ].…”

Pathophysiology and treatment of patients with beta-thalassemia – an update

“…Many antioxidants are supplied by nutrition. A "Mediterranean diet" and moderate wine consumption are thought to have a protective effect ("the French Paradox") due to their high content of antioxidants 56 , 57 . Antioxidants can also be taken as food additives, either as pure compounds, such as vitamins C and E and Q10, or as crude extracts, such as the fermented papaya preparation and curcumin 9 .…”

New Findings on the Effects of Tannic Acid: Inhibition of L‐Type Calcium Channels, Calcium Transient and Contractility in Rat Ventricular Myocytes

“…TA has shown various health beneficial activities (Feldman et al, 2001), and many studies have explored its function and biomedical properties, such as anticancer (Feldman et al, 2001), antibacterial (Chung et al, 1998a), antimutagenic, antioxidant (Nakamura et al, 2003), antidiabetic (Liu et al, 2005), antiinflammatory (Lee et al, 2007) and antihypertensive activities (Turgut Cosan et al, 2015). Our recent study showed that TA could exert cardioprotective effects against isoproterenol-induced myocardial ischemia injury in rat heart (Hu et al, 2015). However, no scientific report has systematically demonstrated cellular and ion channel mechanisms of TA in cardiovascular effects.…”