Cardioprotective Effect of Selenium Against Cyclophosphamide-Induced Cardiotoxicity in Rats

Abstract

The objective of this study is to evaluate the possible protective effects of selenium (Se) against cyclophosphamide (CP)-induced acute cardiotoxicity in rats. A total of 42 male Spraque-Dawley rats were divided into six groups (n = 7). Rats in the first group were served as control. Rats in the second group received CP (150 mg/kg) at the sixth day of experiment. Animals in the third and fourth groups were treated with only 0.5 and 1 mg/kg Se respectively for six consecutive days. Rats in the fifth and sixth groups received respective Se doses (0.5 or 1 mg/kg) for 6 days and then a single dose of CP administered on the sixth day. On day 7, the animals were sacrificed; blood samples were collected to measure malondialdehyde (MDA), glutathione (GSH), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), and ischemia-modified albumin (IMA) levels. Heart tissues were processed routinely and tissue sections were stained with H + E for light microscopic examination. In the CP-treated rats MDA, LDH, CK-MB, and IMA serum levels increased, while GSH levels decreased. Microscopic evaluation showed that tissue damage was conspicuously lower in CP plus Se groups. Moreover, 1 mg/kg Se was more protective than 0.5 mg/kg Se as indicated by histopathological and biochemical values. In conclusion, Se is suggested to be a potential candidate to ameliorate CP-induced cardiotoxicity which may be related to its antioxidant activity.

The Role of Antioxidants in Ameliorating Cyclophosphamide-Induced Cardiotoxicity

“…Gunes et al [72] Male Sprague Dawley rats Animals received respective selenium (Se) doses (0.5 or 1 mg/kg) for 6 days and then a single dose of CP administered on the sixth day. On day 7, the animals were sacrificed.…”

The Efficacy of Squalene in Cardiovascular Disease Risk-A Systematic Review

“…Other pharmacological inductions of heart damage used doxorubicin to induce cardiomyopathy [64] and cyclophosphamide to induce cardiotoxicity [63]. Both doxorubicin and cyclophosphamide are anticancer drugs with cardiotoxicity as their major adverse effect [72,73] and are commonly used to induce cardiotoxicity and heart failure (HF) in animal models [74][75][76][77]. Hypercholesterolemia is commonly induced by high fat or cholesterol diet.…”

The role of selenium in bevacizumab induced cardiotoxicity

“…In a study by Demirci et al, authors showed that se-lenium treatment might have benefi cial effects in oxidative stress, infl ammation and apoptosis (51). Also, it is known that selenium has cardioprotective effects against toxic elements, xenobiotics and viral infections (19).…”

“…In this study, we wanted to evaluate the effect of bevacizumab on calcium signaling, apoptosis and oxidative stress in cardiomyocytes and we wanted to investigate the modulator role of selenium (as an important cardioprotective antioxidant) in bevacizumab induced cardiotoxicity (19).…”