Cardioprotective effect of grape seed proanthocyanidins on isoproterenol-induced myocardial injury in rats

Karthikeyan, Kaliaperumal; Bai, Bing; Devaraj, S. Niranjali

doi:10.1016/j.ijcard.2006.03.016

Cited by 165 publications

(114 citation statements)

References 36 publications

Supporting

Mentioning

100

Contrasting

Order By: Relevance

“…28 In present study treatment with ISO decreased level of GSH which was significantly elevated on treatment with ACFE indicating protection against isoproterenol induced myocardial injury. We observed significant decrease in activity of SOD and Catalase in isoproterenol treated group which were restored to near normal levels by administration of ACFE.…”

Section: Fig 2: Histopathological Changes In Hearts Of Different Groupssupporting

confidence: 57%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

Myocardial infarction develops due to ischemia induced oxidative stress. Averrhoa Carambola fruit (ACF) is reported to possess strong antioxidant activity which may protect the heart against isoproterenol (ISO) induced myocardial infarction (MI) in rats. To evaluate this activity Averrhoa carambolafruit extract (ACFE) (200mg/kg & 400mg/kg, p.o) was administered to male wistar rats (180-250g) for 21days. At the end of this period, all the rats except rats of normal control group were administered ISO 85mg/kg intraperitonially, for 2 consecutive days to induce MI. After 48 hrs, rats were sacrificed and the level of cardiac biomarker enzymes like cardiac troponin-T (cTn-T), creatinine kinase (CK-MB) and lactatedehydrogenase1(LDH 1 ) in serum were estimated. Also antioxidant parameters likeglutathione (GSH), malondialdehyde (MDA), catalase and superoxide dismutase (SOD) were estimated in heart tissue homogenate and histopathology of heart tissue was performed. Our results suggest that pretreatment of animals with ACFE maintain near normal levels of cardiac biomarker enzymes and antioxidant enzymes in both treatment groups (ACFE 200mg/kg, 400mg/kg) when compared to ISO treated group. Histopathological findings of infarcted hearts treated with ACFE showed reduction in necrosis, infiltration of leucocyte and inflammation as compared to isoproterenol treated group.Thus the present study provides experimental evidence that hydro alcoholic extract of Averrhoa carambola fruit possess strong antioxidant properties which protect the myocardium against isoproterenol induced infarction.

show abstract

Section: Fig 2: Histopathological Changes In Hearts Of Different Groupssupporting

confidence: 57%

Untitled

2016

IJPSR

View full text Add to dashboard Cite

show abstract

“…All animals received human care according to the instruction's guidelines of the National Institutes of Health (NIH) and the protocol was approved by the local committee of the Mansoura University. After an acclimatization period of 1 week, animals were divided into four groups, 6 rats each as follow: Group 1, untreated control; Group 2, treated orally with GSPE (100 mg/kg b.w) (Karthikeyan et al 2007); Group 3, treated with GA3 (3.85 mg/Kg body weight) (Celik et al 2007); Group 4, treated with GSPE and GA3 at the same doses of group 2&3. All treatments were given daily using gastric intubation and continued for 2 months.…”

Section: Animal Grouping and Experimental Designmentioning

confidence: 99%

Grape seeds proanthocyanidin extract as a hepatic-reno-protective agent against gibberellic acid induced oxidative stress and cellular alterations

Hassan

Al-Rawi

2012

Cytotechnology

View full text Add to dashboard Cite

The present study aims to investigate the heptonephro-protective effect of grape seeds proanthocyanidin extract (GSPE) against the risks induced by gibberellic acid (GA3) in male rats. The results recorded that GA3 caused a significant increase in total lipids, total cholesterol, triglycerides and LDL-C levels in serum, concomitant with a significant decrease in serum HDL-C. A significant increase in serum AST, ALT, urea and creatinine, while, a significant decrease in total protein content in serum was observed in rats given GA3. Hepatic and renal lipid peroxidation product (MDA) was significantly increased, meanwhile, total antioxidant capacity (TAC), glutathione, and catalase levels were significantly decreased. In addition, there was a negative change in liver structure including dilatation in the central veins with degeneration of endothelium cells and cellular injury around the veins as well as in the kidney structure such as lesion in both glomeruli and tubules, detachment of the Malpighian corpuscles from the Bowman's capsule's epithelium, shrinkage in the glomerular capillary network. However, almost all of these adverse effects seemed to be ameliorated by oral administration of GSPE with GA3 to rats for 2 month indicating the protective effect of grape seeds GSPE on GA3 induced oxidative stress in rats.

show abstract

“…Contrary to several previous studies, our data suggest that ISO did not increase oxidative stress. Karthikeyan, et al, (34) Patel, et al, (35) and Mukherjee, et al (36) reported that ISO causes an increase in oxidative stress markers. However, there were several critical differences between these studies and the current study: (1) animals in the previous studies received repeated doses of ISO, as compared to the single dose administered in the current study, (2) measures of lipid peroxidation and oxidative stress were measured two or more days after the initial dose of ISO, as compared to 24 hours after the initial dose in the current study, (3) all 3 of these studies analysed homogenised myocardium, not circulating plasma.…”

Section: Discussionmentioning

confidence: 99%