Cardioprotection activity and mechanism of Astragalus polysaccharide in vivo and in vitro

Liu, Debin; Chen, Lei; Zhao, Jianye; Cui, Kang

doi:10.1016/j.ijbiomac.2018.01.048

Cited by 44 publications

(28 citation statements)

References 28 publications

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“…Although astragalus polysaccharide and cycloastragenol were not recruited by TCMSP, and targets corresponding to astragalus polysaccharide, cycloastragenol, and astragaloside IV were not obtained from TCMSP, they were also considered the critical compounds of Huangqi for their strong cardioprotective effect. Astragalus polysaccharide was proved to protect myocardium through reducing oxidant stress and cardiomyocyte apoptosis, inhabiting the generation of inflammatory factors like phosphorylated NF-κB, IL-1β, IL-6, TNF-α, and so on, restoring normal autophagic flux [21,[32][33][34][35][36]. In addition, research confirms that cycloastragenol improves cardiac defect and remodeling by enhancing autophagy in myocardial cells and reduces the production of MMP-2 and MMP-9 [24].…”

Section: Discussionmentioning

confidence: 81%

“…A total of 46 nodes and 423 edges were involved in PPI network. e top 5 targets included IL-6 (38), TNF (37), AKT1 (36), VEGFA (35), and NOS3 (33) ( Figure 4). MCODE was used to analyze the most significant module and obtained 25 core target nodes, including the top 5 targets in initial PPI network, which further proved that the importance of IL-6, TNF, AKT1, VEGFA, and NOS3 ( Figure 5).…”

Section: Ppi Network Of Targets' Intersection Of Hf and Huangqimentioning

confidence: 99%

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Exploring Molecular Mechanism of Huangqi in Treating Heart Failure Using Network Pharmacology

Tao

Huang

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Heart failure (HF), a clinical syndrome with a high incidence due to various reasons, is the advanced stage of most cardiovascular diseases. Huangqi is an effective treatment for cardiovascular disease, which has multitarget, multipathway functions. Therefore, we used network pharmacology to explore the molecular mechanism of Huangqi in treating HF. In this study, 21 compounds of Huangqi, which involved 407 targets, were obtained and reconfirmed using TCMSP and PubChem databases. Moreover, we used Cytoscape 3.7.1 to construct compound-target network and screened the top 10 compounds. 378 targets related to HF were obtained from CTD and GeneCards databases and HF-target network was constructed by Cytoscape 3.7.1. The 46 overlapping targets of HF and Huangqi were gotten by Draw Venn Diagram. STRING database was used to set up a protein-protein interaction network, and MCODE module and the top 5 targets with the highest degree for overlapping targets were obtained. GO analysis performed by Metascape indicated that the overlapping targets were mainly enriched in blood vessel development, reactive oxygen species metabolic process, response to wounding, blood circulation, and so on. KEGG analysis analyzed by ClueGO revealed that overlapping targets were mainly enriched in AGE-RAGE signaling pathway in diabetic complications, IL-17 signaling pathway, HIF-1 signaling pathway, c-type lectin receptor signaling pathway, relaxin signaling pathway, and so on. Finally, molecular docking showed that top 10 compounds of Huangqi also had good binding activities to important targets compared with digoxin, which was carried out in CB-Dock molecular docking server. In conclusion, Huangqi has potential effect on regulating overlapping targets and GE-RAGE signaling pathway in diabetic complications, IL-17 signaling pathway, HIF-1 signaling pathway, and so on to be a latent multitarget, multipathway treatment for HF.

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Section: Discussionmentioning

confidence: 81%

Section: Ppi Network Of Targets' Intersection Of Hf and Huangqimentioning

confidence: 99%

Exploring Molecular Mechanism of Huangqi in Treating Heart Failure Using Network Pharmacology

Tao

Huang

Wang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…APS can increase the expression of Toll-like receptor 4 to enhance innate immunity during mucosal bacterial infections in vivo and in vitro ( 37 ). It has also been used as an adjuvant for an avian infectious bronchitis virus vaccine ( 38 ), it inhibits cell volume increases in the myocardium, and has a protective effect on myocardial ischemia and reperfusion injury/isoprenaline-treated cardiomyocytes by inhibiting myocardial tissue apoptosis in vivo ( 39 ). However, APS treatment inhibited proliferation and promoted apoptosis in H460 NSCLC cells ( 36 ), and combination treatment with vinorelbine and cisplatin administered to patients with advanced NSCLC significantly improved quality of life ( 40 ).…”

Section: Discussionmentioning

confidence: 99%

Astragalus polysaccharide combined with 10-hydroxycamptothecin inhibits metastasis in non-small cell lung carcinoma cell lines via the MAP4K3/mTOR signaling pathway

Zhou

Hong

et al. 2018

Int J Mol Med

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Non-small cell lung carcinoma (NSCLC) is a life-threatening malignancy. The level of the cell growth regulator mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3) has been shown to be correlated with a high risk of NSCLC recurrence and poor recurrence-free survival rate. The present study examined the effects of Astragalus polysaccharide (APS) and 10-hydroxycamptothecin (HCPT), which are associated with marked suppression and dephosphorylation of the MAP4K3/mammalian target of rapamycin (mTOR) signaling pathway, in the H1299 NSCLC cell line. APS and HCPT decreased H1299 cell viability, induced apoptosis and altered the cell cycle stages, as evaluated using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometric analysis. Furthermore, APS increased the expression of apoptosis-associated genes B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (BAX), of proteases cysteine-aspartic acid protease (caspase)-3 and -9, and of cytochrome c. HCPT promoted autophagy in H1299 cells, with concomitant suppression of the expression of MAP4K3 and downregulation of mTOR signaling. Notably, combination treatment with the two agents reduced the migration and invasion of H1299 cells compared with the single treatments. It was also demonstrated that the overexpression of MAP4K3 promoted the migration and invasion of H1299 cells, and that the kinase activity was essential to this. These findings suggested that MAP4K3 may be an attractive target for the treatment of NSCLC

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“…Huoxiang [24] and Mahuang [25] have certain antiviral and anti-inflammatory effects. Huangqi [26,27], Dangshen [28,29], Baizhun [30], and Fuling [31,32] can enhance immunity and improve failure of organs, such as the heart and the brain, caused by ischemia and hypoxia. We also discovered an effective CHM against COVID-19 by using the TCMATCOV platform in another study [33].…”

Section: Discussionmentioning

confidence: 99%

Chinese herbal medicine reduces mortality in patients with severe and critical Coronavirus disease 2019: a retrospective cohort study

Chen

Yang

et al. 2020

Front. Med.

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This study aimed to evaluate the efficacy of Chinese herbal medicine (CHM) in patients with severe/ critical coronavirus disease 2019 (COVID-19). In this retrospective study, data were collected from 662 patients with severe/critical COVID-19 who were admitted to a designated hospital to treat patients with severe COVID-19 in Wuhan before March 20, 2020. All patients were divided into an exposed group (CHM users) and a control group (non-users). After propensity score matching in a 1:1 ratio, 156 CHM users were matched by propensity score to 156 non-users. No significant differences in seven baseline clinical variables were found between the two groups of patients. All-cause mortality was reported in 13 CHM users who died and 36 non-users who died. After multivariate adjustment, the mortality risk of CHM users was reduced by 82.2% (odds ratio 0.178, 95% CI 0.076-0.418; P < 0.001) compared with the non-users. Secondly, age (odds ratio 1.053, 95% CI 1.023-1.084; P < 0.001) and the proportion of severe/critical patients (odds ratio 0.063, 95% CI 0.028-0.143; P < 0.001) were the risk factors of mortality. These results show that the use of CHM may reduce the mortality of patients with severe/ critical COVID-19.

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Cardioprotection activity and mechanism of Astragalus polysaccharide in vivo and in vitro

Cited by 44 publications

References 28 publications

Exploring Molecular Mechanism of Huangqi in Treating Heart Failure Using Network Pharmacology

Exploring Molecular Mechanism of Huangqi in Treating Heart Failure Using Network Pharmacology

Astragalus polysaccharide combined with 10-hydroxycamptothecin inhibits metastasis in non-small cell lung carcinoma cell lines via the MAP4K3/mTOR signaling pathway

Chinese herbal medicine reduces mortality in patients with severe and critical Coronavirus disease 2019: a retrospective cohort study

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