Cardiomyocytes generating spontaneous Ca2+-transients as tools for precise estimation of sarcoplasmic reticulum Ca2+ transport

Maltsev, Alexander V.; Kokoz, Yu. M.

doi:10.1016/j.abb.2020.108542

Cited by 7 publications

(2 citation statements)

References 67 publications

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“…54 After VFBP fusing with the cardiomyocyte membrane, the effect of VFBP-mediated K + ion transport on excitation−contraction coupling of cardiomyocytes was studied (see the Methods section). 55,56 The fluorescence changes detected in cardiomyocytes loaded with calcium fluorescence indicators during the rapid release of Ca 2+ across calcium ion channels are called calcium sparks. Images of calcium sparks captured by the confocal laser scanning microscope showed that the frequency of calcium sparks reduced and the contraction amplitude, full width at halfmaximum (fwhm), full duration at half-maximum (FDHM), and time to peak of calcium sparks increased after the damage of K + channels in cardiomyocytes (Figures 4b and S29−S35).…”

Section: ■ Resultsmentioning

confidence: 99%

Bionic Potassium Ion Channel in Live Cells Repairs Cardiomyocyte Function

Shen,

Lu,

Peng

et al. 2024

J. Am. Chem. Soc.

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The disturbance of potassium current in cardiac myocytes caused by potassium channel dysfunction can lead to cardiac electrophysiological disorders, resulting in associated cardiovascular diseases. The emergence of artificial potassium ion channels opens up a way to replace dysfunctional natural ion channels and cure related diseases. However, bionic potassium ion channels have not been introduced into living cells to regulate cell function. One of the biggest challenges is that when the bionic channel fuses with the cell, it is difficult to control the inserting angle of the bionic potassium channel to ensure its penetration of the entire cell membrane. In nature, the extracellular vesicles can fuse with living cells with a completely preserved structure of vesicle protein. Inspired by this, we developed a vesicle fusion-based bionic porin (VFBP), which integrates bionic potassium ion channels into cardiomyocytes to replace damaged potassium ion channels. Theoretical and experimental results show that the inserted bionic ion channels have a potassium ion transport rate comparable to that of natural ion channels, which can restore the potassium ion outflow in cardiomyocytes and repair the abnormal action potential and excitation−contraction coupling of cardiomyocytes. Therefore, the bionic potassium ion channel system based on membrane fusion is expected to become the research object in many fields such as ultrafast ion transport, transmembrane delivery, and channelopathies treatment.

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Section: ■ Resultsmentioning

confidence: 99%

Bionic Potassium Ion Channel in Live Cells Repairs Cardiomyocyte Function

Shen,

Lu,

Peng

et al. 2024

J. Am. Chem. Soc.

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“…The generation and modulation of the concentration of cytoplasmic calcium [Ca 2+ ] i oscillations in neurons involve inotropic GABA(A)R, NMDAR, and CP-AMPAR receptors, as well as signaling systems that ensure the release of calcium from the ER [264,265,[274][275][276][277]. Oscillations in calcium and other signaling molecules in electronically non-excitable cells are currently being actively studied: endocrine cells, glia, cardiomyocytes, adipocytes, and endotheliocytes [12,262,264,266,270,274,288].…”

Section: Mechanisms Of Generation Of Oscillations In the Concentratio...mentioning

confidence: 99%

Application of Spectral Methods of Analysis for Description of Ultradian Biorhythms at the Levels of Physiological Systems, Cells and Molecules (Review)

2023

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The presence of biological rhythms is a characteristic of all living organisms. Over the past 60 years, scientists around the world have accumulated a huge amount of data on rhythmic processes in living systems at various levels. The acquired knowledge has found applications in human economic activity and medicine. The ultradian (less than a day) rhythms at the organismal, organ, and cellular levels are characterized by high diversity. Unfortunately, biorhythms in different systems are considered, most often, in isolation from each other. Much knowledge about biorhythms was obtained using expert evaluation methods, and later methods of spectral analysis were used to describe biorhythms. Ultradian rhythms have a relatively short duration; therefore, they can be characterized by spectral analysis methods. More and more researchers believe that in order to further expand the understanding of the nature and purpose of biorhythms, the use of more advanced methods of mathematical processing is required, and rhythms in different organs, tissues, and cells should be considered parts of a single system. This review is intended to provide the reader with the variety of ultradian rhythms in living systems (organismal, organ, cellular, molecular levels), the mechanisms of their generation, and their functions to give the reader a picture of the possible relationships between these rhythms. Further, the reader will be able to get acquainted with the variety of mathematical methods for analyzing biorhythms, including bispectral and cross-correlation analyses.

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