Cardioinhibitory Properties of Potato Glycoalkaloids in Beetles

Abstract

The semi-isolated heart bioassay was used to evaluate the effect of glycoalkaloids extracted from potato leaves on the heart contractile activity of three beetle species Zophobas atratus, Tenebrio molitor and Leptinotarsa decemlineata. The dose-response curves indicated species specific action of tested substances. Application of glycoalkaloids on the continuously perfused Z. atratus heart inhibited progressively frequency contractions; higher concentrations exerted short and reversible cardiac arrests. In the rest two beetle species tested glycoalkaloids caused no cardiotropic effect. In vivo bioassay with 1 day old Z. atratus pupae showed that the extract induces a negative inotropic effect on the heart.

Biological activity of Melia azedarach extracts against Spodoptera exigua

“…Previously, we showed that glycoalkaloid extract prepared from potato leaves significantly decreases the heartbeat frequency of adults and pupae of the beetle Zophobas atratus (F., 1775) (Marciniak et al 2010). Glycoalkaloids decrease the insect heartbeat in similar concentration as extract used in this study.…”

“…Heart activity (in vivo) bioassay was carried out by non-invasive optoelectronical method as described previously (Marciniak et al 2010;Slama & Rosinski 2005). The heart activity was monitored for 24 hours after topical application of CRU and LIM at the concentration of 1% w/v on 0-and 1-day old pupae of S. exigua.…”

New physiological activities of myosuppressin, sulfakinin and NVP-like peptide in Zophobas atratus beetle

“…Heart rhythm of pupae was manifested by the regular alternations of the forward orientated (anterograde), the backward orientated (retrograde) cardiac pulsations together with diastasis as described previously (Marciniak et al 2010a). The heartbeat frequency in control insects showed on average 30 beats/min in anterograde phase and 12 beats/min in retrograde phase.…”

“…The in vivo heart bioassay was conducted on 1 day old Z. atratus pupae by means of noninvasive optocardiographic technique of Slama and Rosinski (Slama and Rosinski 2005) as described previously (Marciniak et al 2010a). The heart rhythm was recorded for 24 h. Sample injections were performed after 2 h of initial stabilization of pupae heartbeat rhythm.…”

POTATO LEAF EXTRACT AND ITS COMPONENT, α‐SOLANINE, EXERT SIMILAR IMPACTS ON DEVELOPMENT AND OXIDATIVE STRESS IN Galleria mellonella L.

“…However, leaves, unripe fruits, and flowers have the highest GA concentrations (Friedman, 2006). Potato GAs make up a plant defense mechanism (Friedman, 2006;Adamski et al, 2009;Marciniak et al, 2010;Büyükgüzel et al, 2013), and although knowledge regarding their activities on insect physiology and mechanisms of action is still limited (Krishnan and Kodrik, 2006), crude plant extracts may exert a greater effect than the individual allelochemicals due to possible synergisms of multiple compounds (Nenaah, 2011a;Ventrella et al, 2014). This led us to pose the hypothesis that the dried, total extracts of fresh potato leaves (EPL) exert more deleterious impact on wax moth larvae than pure α-solanine, a major GA component of potato leaves.…”