Cardiogel supports adhesion, proliferation and differentiation of stem cells with increased oxidative stress protection

Sreejit, P.; Rs, Verma

doi:10.22203/ecm.v021a09

Cited by 26 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ex‐4 preconditioning protects MSCs against H 2 O 2 ‐induced apoptosis in a dose‐dependent manner, and pigment epithelium‐derived factor (PEDF) increases MSC survival rates in long‐term culture by attenuating p53 and p16 signalling . In addition, various types of extracellular matrix components including decellularized cell‐deposited extracellular matrix and cardiogel derived from cardiac fibroblast strongly promote the self‐renewal and differentiation of MSCs by up‐regulating the expression of intracellular antioxidative enzymes and maintaining low levels of ROS even under excessive H 2 O 2 conditions in vitro . In addition, Lai et al reconstructed a native extracellular matrix which is consisted of collagen, fibronectin, small leucine‐rich proteoglycans and major components of basement membrane to maintain the cell activities of MSCs via downregulation of ROS level in vitro.…”

Section: Strategies To Control Ros Levels For Msc Fates In Vitro and mentioning

confidence: 99%

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Zhao

Peng

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are broadly used in cell‐based regenerative medicine because of their self‐renewal and multilineage potencies in vitro and in vivo. To ensure sufficient amounts of MSCs for therapeutic purposes, cells are generally cultured in vitro for long‐term expansion or specific terminal differentiation until cell transplantation. Although physiologically up‐regulated reactive oxygen species (ROS) production is essential for maintenance of stem cell activities, abnormally high levels of ROS can harm MSCs both in vitro and in vivo. Overall, additional elucidation of the mechanisms by which physiological and pathological ROS are generated is necessary to better direct MSC fates and improve their therapeutic effects by controlling external ROS levels. In this review, we focus on the currently revealed ROS generation mechanisms and the regulatory routes for controlling their rates of proliferation, survival, senescence, apoptosis, and differentiation. A promising strategy in future regenerative medicine involves regulating ROS generation via various means to augment the therapeutic efficacy of MSCs, thus improving the prognosis of patients with terminal diseases.

show abstract

Section: Strategies To Control Ros Levels For Msc Fates In Vitro and mentioning

confidence: 99%

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Zhao

Peng

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…The osteogenic cell (MC3T3-E1)-derived ECM, attached to the plates, promotes the osteogenic differentiation of embryonic stem cells seeded on the ECM (Evans et al 2010). It has been demonstrated that cardiac fibroblast-ECM supports early maturation of ES-derived cardiomyocytes in vitro (Baharvand et al 2005), and improves proliferate and cellular adhesion of BMSCs (Sreejit and Verma 2011). …”

Section: Introductionmentioning

confidence: 99%

Proliferative effect and osteoinductive potential of extracellular matrix coated on cell culture plates

et al. 2013

View full text Add to dashboard Cite

Different cell/tissue derived extracellular matrix (ECM) display subtle differences that might provide important cues for proliferation and differentiation of cells in vitro or in vivo. However, the bioactivities of different ECMs in vitro were not fully understood. In this study, osteoblasts-derived and fibroblast-derived ECM-coated cell culture dishes were prepared respectively by culturing osteoblastic MC3T3-E1 cells and rat fibroblast then decellularizing the cultures. We investigated the bioactivities of the two different ECMs coated on cell culture plates using cellular, biochemical and molecular method. The proliferative activity of the bone marrow-derived mesenchymal stem cells (BMSCs) cultured on osteoblast-ECM was lower than for BMSCs grown on fibroblast-ECM. Compared with the BMSCs cultured on fibroblast-derived ECM, the cells grown on osteoblastic ECM showed enhanced alkaline phosphatase (ALP) activity, higher BMP-2 and osteopontin protein levels, increased secreted calcium content, and higher levels of runt-related transcriptional factor 2 (Runx 2) and osteocalcin (OCN) mRNA. Knockdown of BMP-2 or FGF-2 with shRNA transfection hardly effected osteoblastic differentiation or proliferation of MC3T3-E1 seeded on osteoblast-ECM or fibroblast-ECM. Therefore, the osteoblastic ECM had better osteoinductive potential and lower proliferative effect than fibroblastic ECM, and the two ECM presented enough bioactivity, knockdown of growth factors had no significant effect on differentiation and proliferation of re-seeded cells.

show abstract

“…6) Recently, the bioactivity of CF-derived ECM has been investigated in vitro. [11][12][13] However, the effects of CF-derived ECM on the metabolism, growth, and function of heart cells are not fully understood.…”

Section: Discussionmentioning

confidence: 99%

Cardiac Fibroblast-Derived Extracellular Matrix Produced <I>In Vitro</I> Stimulates Growth and Metabolism of Cultured Ventricular Cells

et al. 2013

View full text Add to dashboard Cite

SummaryCardiac fi broblasts (CFs) produce extracellular matrix (ECM) which is a potent regulator of heart cell function and growth, and provides a supportive microenvironment for heart cells. Therefore, CF-derived ECM produced in vitro is very suitable for heart-cell culturing and cardiac tissue engineering. The aim of this study was to investigate the effect of CF-derived ECM produced in vitro on the growth and metabolism of cultured ventricular cells. CF-derived ECM-coated cell culture dishes were prepared by culturing rat CFs and then decellularizing the cultures. Isolated neonatal rat ventricular cells were seeded on ECM-coated, collagen I-coated or uncoated dishes, and the growth of cells after 1-5 days of culture was assayed with MTT reagent. In addition, cellular metabolic activity was analyzed by spectrophotometric methods and protein levels of sarco(endo)plasmic reticulum Ca 2+ -ATPase type 2a (SERCA2a) by Western blotting. The relative growth of ventricular cells was better on ECM-coated than on uncoated or collagen I-coated dishes. Furthermore, the glucose consumption ratio, lactic acid production ratio, Na + /K + -ATPase activity, SERCA activity and protein levels of SERCA2a were all higher in cells on the ECM-coated dishes. In conclusion, cardiac fi broblast-derived ECM produced in vitro stimulates the growth and metabolism of cultured ventricular cells. This study indicates that the bioactivity of the ECM supports heart cell growth in vitro, and this might be useful for cardiac tissue engineering. (Int Heart J 2013; 54: 40-44)

show abstract

Cardiogel supports adhesion, proliferation and differentiation of stem cells with increased oxidative stress protection

Cited by 26 publications

References 34 publications

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Regulation of the mitochondrial reactive oxygen species: Strategies to control mesenchymal stem cell fates ex vivo and in vivo

Proliferative effect and osteoinductive potential of extracellular matrix coated on cell culture plates

Cardiac Fibroblast-Derived Extracellular Matrix Produced <I>In Vitro</I> Stimulates Growth and Metabolism of Cultured Ventricular Cells

Contact Info

Product

Resources

About