Cardiac Troponins: Clinical and Analytical Aspects

Sodi, Ravinder

doi:10.1016/s0065-2423(05)41003-3

Cited by 4 publications

(3 citation statements)

References 260 publications

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“…5,9,11,12,15 Conventional enzyme-linked immunosorbent assays (ELISA) are most frequently used for quantitative detection of TnI, and the commercially available sensors have limits of detection (LOD) ranging from 0.001 to 0.1 ng/mL. 9,[16][17][18] Other TnI sensors have been reported using fluorescence resonance energy transfer (FRET; LOD, 1-800 ng/mL) 19,20 and electrochemical techniques such as anodic stripping voltammetry and cyclic voltammetry (LOD, 0.1-0.5 ng/mL). 21,22 These techniques rely on antibodies for biomolecular recognition and require multistep processing and labeling of the samples.…”

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confidence: 99%

Development of a Troponin I Biosensor Using a Peptide Obtained through Phage Display

Cropek

West

et al. 2010

Anal. Chem.

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A small synthetic peptide with nanomolar affinity for cardiac troponin I (TnI), previously identified from a polyvalent phage displayed library, has been immobilized on a gold surface for TnI detection. The binding affinity of gold-immobilized peptides for TnI was studied and compared with that of phage-immobilized peptides. Quartz crystal microbalance (QCM), cyclic voltammetry, and electrochemical impedance spectroscopy (EIS) were used to monitor both the immobilization and target binding processes. All three techniques show that the binding is specific for TnI as compared to a streptavidin (SA) control. The response curves obtained at TnI concentrations ranging from 0 to 10 μg/mL, using both QCM and EIS, were also compared. For the EIS measurements, the sensitivity was 0.30 ± 0.030 normalized impedance/(μg/mL) and the limit of detection (LOD) was 0.34 μg/mL. Using the QCM, a sensitivity of 18 ± 1 Hz/(μg/mL) was obtained, corresponding to an LOD of 0.11 μg/mL. Although the QCM demonstrated a lower LOD as compared to EIS, the latter technique exhibited a larger linear dynamic range than QCM. In a relevant tissue culture milieu, Minimum Essential Media (MEM), the sensitivity of the EIS measurement was greater than that obtained in a phosphate buffer system (PBS). The kinetics of target binding using QCM were analyzed by two independent methods, and the dissociation constants (K(D) = 66 ± 4 nM and 17 ± 8 nM) were an order of magnitude higher than that calculated for the polyvalent phage particles (K(D) = 2.5 ± 0.1 nM). Even though the affinity of the immobilized peptides for TnI was somewhat reduced, overall, these results demonstrate that peptides obtained from the biopanning of phage display libraries can be readily used as sensing probes in biosensor development.

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confidence: 99%

Development of a Troponin I Biosensor Using a Peptide Obtained through Phage Display

Cropek

West

et al. 2010

Anal. Chem.

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“…Cardiac troponins are the most sensitive and specific markers for the conclusive detection of myocardial infarction and acute coronary syndromes and are deemed the gold standard indicator of myocardial necrosis. 44 Only eight of this study's participants had positive troponin levels. So far, the association between phthalate exposure and myocardial damage has been supported only by one study.…”

Section: Discussionmentioning

confidence: 83%

“…This study detected no significant difference between cardiac troponin and MEHP levels. Cardiac troponins are the most sensitive and specific markers for the conclusive detection of myocardial infarction and acute coronary syndromes and are deemed the gold standard indicator of myocardial necrosis . Only eight of this study’s participants had positive troponin levels.…”

Section: Discussionmentioning

confidence: 88%

Phthalate Exposure and Coronary Heart Disease: Possible Implications of Oxidative Stress and Altered miRNA Expression

Moawad,

Awady,

Ali

et al. 2024

Chem. Res. Toxicol.

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The relationship between phthalate exposure and coronary heart disease (CHD) is still unclear. This study aimed to investigate the association between phthalate exposure and CHD and determine the possible atherogenic mechanisms of phthalates by assessing oxidative stress and altering miRNA expression. This case-control study included 110 participants (55 CHD patients and 55 healthy controls). The levels of oxidative stress markers, malondialdehyde (MDA), and superoxide dismutase (SOD), and the expression of miRNA-155 (miR-155) and miRNA-208a (miR-208a), were measured and correlated with the urinary mono-2ethylhexyl phthalate (MEHP). Highly significant differences were detected between the CHD cases and the control group regarding MEHP, MDA, SOD, miR-155, and miR-208a (p-value < 0.001). Spearman correlations revealed a significant positive correlation between MDA and MEHP in urine (P = 0.001 and rs = 0.316) and a significant negative correlation between SOD and MEHP in urine (P < 0.001 and rs = −0.345). Furthermore, significant positive correlations were observed between miR-155 and urinary MEHP (P = 0.001 and rs = 0.318) and miR-208a and urinary MEHP (P < 0.001 and rs = −0.352). This study revealed an association between phthalate exposure, as indicated by urinary MEHP and CHD; altered expression of miR-155 and miR-208a and oxidative stress could be the fundamental mechanisms.

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