Cardiac troponin-T and CK-MB (mass) levels in cardiac and non cardiac disease

Reddy, G. C.; Kusumanjali, G.; Sharada, A. H. R.; Rao, P Viswajyothi

doi:10.1007/bf02894263

Cited by 9 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Contrary to others opinion that cTnT may be more sensitive to detect minor infarction which could not be detected by CK-MB (20,21), our study reveals only 22% cTnT positive cases while it was over 46% (22) and 90 % (23) in other studies; Although cTn-T positive cases had higher CK-MB (Table 3). The reason for only 22 % positive cTnT in our study is difficult to explain.…”

Section: Resultscontrasting

confidence: 99%

Diagnosis of acute myocardial infarction: CK-MB versus cTn-T in Indian patients

Gupta

Singh

Bapat

et al. 2008

Indian J Clin Biochem

View full text Add to dashboard Cite

The comparative diagnostic efficacy of two cardiac markers : CK-MB and cTn-T , has scarcely been investigated in Indian patients of acute myocardial infarction. The present study was conducted for the same objective. The present study comprised of 59 patients . Males were 44 (75%) and females were 15 (25 %). The age of patients ranged from 32-84 years with mean age of 62.8 yrs. The mean age of males and females were 60 and 63 yrs respectively. All patients presented with history of chest pain with a 12 leads ECG proven MI (ST Elevation, discordant T-waves). CK-MB was estimated in peripheral blood samples at 0,24,48 and 72 hours by an autoanalyzer. Following 12 hours of admission bed side Troponin -T test was done employing cTn-T marker kit. Initially (0 hr), in 50% patients CK-MB was elevated. By end of 24 hours all the patients were CK-MB positive and peak level was attained at 24 hrs. Then it tended to decline over next 48 hrs. There were no false positive or negative results. The cTn-T test was positive only in 22 % of ECG positive infarctions. However, the cTn-T positive cases were always accompanied by a higher CK-MB levels. A significantly lower cTn-T positive cases in Indian patients can only be attributed to some difference in amino acid sequence of Indian cTn-T and occidental cTn-T. A larger study from other Indian cardiac centers can either substantiate or contradict our results.

show abstract

Section: Resultscontrasting

confidence: 99%

Diagnosis of acute myocardial infarction: CK-MB versus cTn-T in Indian patients

Gupta

Singh

Bapat

et al. 2008

Indian J Clin Biochem

View full text Add to dashboard Cite

show abstract

“…The increase of cardiac Troponin T was 20 times higher than the normal blood donors in AMI at the time of admission whereas that of CKMB was around two and half times in comparison to normal blood donors (17). Our values of CTnT are in agreement with a very recent study (18).…”

Section: Resultssupporting

confidence: 93%

A serial follow up study of cardiac marker enzymes during the week after acute myocardial infarction

Shah

Haridas

2007

Indian J Clin Biochem

View full text Add to dashboard Cite

show abstract

“…The detection limit of the chronoamperometric method was determined as 13 ng mL −1 using the formula DL = 2×RMSD/S. The CK-MB levels can increase from (4±2) ng mL −1 to values as high as 400 ng mL −1 as a consequence of severe myocardial injury [2, 11]. Although this DL is higher than the CK-MB levels in healthy individuals, the sensor could be used to quantify CK-MB in pathological samples.…”

Section: Discussionmentioning

confidence: 99%

“…The concentrations of these proteins in blood depend on the severity of AMI and the delay time after the onset of AMI [2, 3, 10, 11]. The existing POCT devices for cardiac proteins generally employ immunochromatographic separation and spectrophotometric or spectrofluorometric detection systems [12, 13].…”

Section: Introductionmentioning

confidence: 99%

Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

et al. 2010

View full text Add to dashboard Cite

This article presents a simple chronoamperometric immunosensor for the quantitative assessment of creatine kinase MB (CK-MB) in 50 µL undiluted serum samples. The immunosensor consists of gold working and counter electrodes patterned onto a glass chip by thin-film photolithography and an external Ag|AgCl reference electrode. The detection limit (DL) of the chronoamperometric method is 13 ng mL −1 (DL = 2×RMSD/S, where RMSD is the residual mean standard deviation of the measured points around a calibration curve with a slope of S). In spiked serum samples, the response was linear up to 300 ng mL −1 of CK-MB. A surface plasmon resonance (SPR) system with simultaneous electrochemical detection (EC-SPR) aided the development of the sandwich immunoassay. Real-time monitoring of the SPR signal was used to optimize the capture antibody immobilization, CK-MB and detection antibody binding, as well as to minimize the nonspecific adsorption of serum proteins to the sensor surface. The detection antibody has been labeled with alkaline phosphatase (ALP) enzyme for sensitive electrochemical detection. ALP catalyzes the hydrolysis of ascorbic acid phosphate and generates ascorbic acid, which is measured chronoamperometrically. The electrochemical immunoassay for CK-MB was less sensitive to nonspecific adsorption related interferences, had a better detection limit, and required a lower volume of sample than the SPR method.

show abstract

Cardiac troponin-T and CK-MB (mass) levels in cardiac and non cardiac disease

Cited by 9 publications

References 12 publications

Diagnosis of acute myocardial infarction: CK-MB versus cTn-T in Indian patients

Diagnosis of acute myocardial infarction: CK-MB versus cTn-T in Indian patients

A serial follow up study of cardiac marker enzymes during the week after acute myocardial infarction

Surface plasmon resonance aided electrochemical immunosensor for CK-MB determination in undiluted serum samples

Contact Info

Product

Resources

About