Cardiac tissue engineering: a reflection after a decade of hurry

Felice, Valentina Di; Barone, Rosario; Nardone, Giorgia; Forte, Giancarlo

doi:10.3389/fphys.2014.00365

Cited by 7 publications

(8 citation statements)

References 30 publications

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“…The most common cell source in clinical applications is still progenitor bone marrow cells, however, long‐term results of their application are yet questionable and more investigations might be required to confirm the safety and efficiency of their application . On the other hand, investigations on the application of MSCs are encouraging as they demonstrate the ability of MSCs in tissue healing, since they have shown the ability to achieve contractile phenotype …”

Section: Discussionmentioning

confidence: 99%

“…10 On the other hand, investigations on the application of MSCs are encouraging as they demonstrate the ability of MSCs in tissue healing, since they have shown the ability to achieve contractile phenotype. 11,12 In some studies, it has been observed that injecting cells into the injured site is not efficient since 90% of the injected cells will enter circulation. 13,14 In a study by Tano et al myocardial function, cell survival and tissue regeneration were compared following cell therapy through intra-myocardial injection and epicardial cell-sheet implantation (scaffold free).…”

Section: Discussionmentioning

confidence: 99%

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Application of tissue‐engineered pericardial patch in rat models of myocardial infarction

Kameli

Khorramirouz

Eftekharzadeh

et al. 2018

J Biomedical Materials Res

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Myocardial infarction (MI) is a major cause of mortality and morbidity in industrialized societies. Myocardial tissue engineering is an alternative and promising approach for substituting injured myocardium through development and seeding of appropriate scaffolds. In this study, we investigated the efficacy of using an acellular pericardium to deliver autologous mesenchymal stem cells (MSCs) to the infarcted site for regeneration of the myocardium. MI was induced in two groups of rats; G1 or MI group, and G2 or patch-implanted group. In G2 group, rats had undergone transplantation of a pericardial patch which was previously seeded with adipose tissue derived MSCs. To evaluate the efficacy of the pericardial patches, biopsies were taken one month after transplantation. In order to evaluate the extent of regeneration, inflammation and fibrosis, histopathological investigations including hematoxylin and eosin (H&E), Sirius Red and trichrome staining were performed. In addition, immunohistochemical investigations by Desmin as well as CD68, CD45 and CD34 antibodies were performed. Furthermore, Tunnel assay was performed to detect the extent of apoptosis. H&E assessments of biopsies from the patch-implanted group confirmed presence of pre-seeded pericardium containing MSCs along with neo-vessels. Immunohistochemical assessments demonstrated higher number of CD34 positive cells and Desmin-positive cells in the patch implanted group (p < 0.05); these findings are suggestive of cardiomyocyte regeneration in G2 rats. This study demonstrates the advantages of application of natural acellular scaffolds as cell delivery devices and it emphasizes neovascularization following this approach. However, further investigations are required to analyze long-term cardiac function in recipients. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2670-2678, 2018.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Application of tissue‐engineered pericardial patch in rat models of myocardial infarction

Kameli

Khorramirouz

Eftekharzadeh

et al. 2018

J Biomedical Materials Res

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“…5 Various techniques for MTE have been investigated, all of which are trying to create a viable contractile cardiac muscle with mechanical and electrical properties similar to those of native myocardium. [6][7][8][9] There are four major approaches to forming contractile cardiac muscle in MTE: prefabricated scaffolds, cell sheet based methods, fabrication of vascularized myocardial tissues and hydrogel techniques. 10 The hydrogel technique was the first method of MTE developed by Eschenhagen et al in 1994.…”

Section: Introductionmentioning

confidence: 99%

Application of minimally invasive injectable conductive hydrogels as stimulating scaffolds for myocardial tissue engineering

Ketabat

Khorshidi

Karkhaneh

2018

Polymer International

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So far, several methods for myocardial tissue engineering have been developed to regenerate myocardium and even create contractile heart muscles. Among these approaches, hydrogel based methods have attracted much attention due to their ability to mimic the architecture of native extracellular matrix. Injectable hydrogels are a specific class of hydrogels which can be formed in situ by physical and/or chemical crosslinking. Generally, using these hydrogels is more advantageous because they are minimally (less) invasive in comparison with open surgery. Moreover, with respect to the fact that ‘myocardium is a conductive tissue’, utilization of conductive polymers for myocardial tissue engineering has demonstrated promising results. Both the injectable hydrogels and conductive polymers have some merits and demerits, but studies show that using a combination of them has prominently enhanced regeneration of the myocardium. In this review, the focus is on injectable hydrogels, conductive polymers and injectable conductive hydrogels for myocardial tissue engineering. © 2018 Society of Chemical Industry

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“…In particular, for the skeletal and cardiac muscles the translational potential of tissue engineering approaches has clearly been shown, even though the construction of these tissues lags behind others given the hierarchical, highly organized architecture of striated muscles. Failure of the cardiac tissue leads to cardiovascular diseases, which are the leading cause of death in the developed world (Di Felice et al, 2014). On the other hand, there are many clinical cases where the loss of skeletal muscle due to a traumatic injury, an aggressive tumor, or prolonged denervation may be cured by the regeneration of the muscle tissue .…”

mentioning

confidence: 99%

Biomaterials and Bioactive Molecules to Drive Differentiation in Striated Muscle Tissue Engineering

2016

Frontiers Research Topics

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Cardiac tissue engineering: a reflection after a decade of hurry

Cited by 7 publications

References 30 publications

Application of tissue‐engineered pericardial patch in rat models of myocardial infarction

Application of tissue‐engineered pericardial patch in rat models of myocardial infarction

Application of minimally invasive injectable conductive hydrogels as stimulating scaffolds for myocardial tissue engineering

Biomaterials and Bioactive Molecules to Drive Differentiation in Striated Muscle Tissue Engineering

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