Cardiac stem cells in the real world

Pouly, Julia; Bruneval, Patrick; Mandet, Chantal; Proksch, Susanne; Peyrard, Séverine; Amrein, C.; Bousseaux, Véronique; Guillemain, R.; Deloche, A; Fabiani, Jean‐Noël; Menasché, Philippe

doi:10.1016/j.jtcvs.2007.10.024

Cited by 112 publications

(83 citation statements)

References 20 publications

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“…Sheep CPC that are c-kit+Isl1+ and c-kit-isl1+ are present and clonable in normal sheep. Interestingly, sheep CPC can co-express c-kit and Isl1 whereas, a CPC population expressing c-kit and Isl1 has not been identified in humans [24,29]. As some c-kit+ cells also may be mast cells [29], the frequent co-expression of Isl1 and c-kit in our study indicates that c-kit positive cells in the sheep heart are cardiovascular progenitors.…”

Section: Discussionmentioning

confidence: 54%

“…Interestingly, sheep CPC can co-express c-kit and Isl1 whereas, a CPC population expressing c-kit and Isl1 has not been identified in humans [24,29]. As some c-kit+ cells also may be mast cells [29], the frequent co-expression of Isl1 and c-kit in our study indicates that c-kit positive cells in the sheep heart are cardiovascular progenitors. Pouly et al identified c-kit+CD45+ cells from human endomyocardial biopsies that do not express the stem cell markers MDR-1, Isl1 or CD105 [29], whereas, Koninckx et al identified c-kit+CD105+ cells that were CD45 negative [30].…”

Section: Discussionmentioning

confidence: 54%

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Isolation, Characterization, and Spatial Distribution of Cardiac Progenitor Cells in the Sheep Heart

Hou

Appleby²,

Fuentes³

et al. 2013

J Clin Exp Cardiolog

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Background: Laboratory large animal models are important for establishing the efficacy of stem cell therapies that may be translated into clinical use. The similarity of ovine and human cardiovascular systems provides an opportunity to use the sheep as a large animal model in which to optimize cell-based treatments for the heart. Recent clinical trials in humans using endogenous cardiovascular progenitor cells report significant improvement in cardiac function following stem cell-based therapy. To date, however, endogenous cardiovascular progenitor cells have not been isolated from the sheep heart.Methods: Cardiovascular cells expressing SSEA-4, CD105 and c-kit were isolated by flow cytometry and cloned from the right atrium of neonatal sheep. The expression of GATA-4, c-kit, and Isl1 was identified by PCR in the cloned cells. Immunohistochemical staining was used to compare the number of SSEA-4 positive cells in the right auricle, right atrium, left ventricle and the apex of the heart of fetal, neonatal and adult sheep. The number of SSEA4+cells was also compared in fetal, pregnant and non-pregnant adult sheep. Results:Four distinct cardiac progenitor cell sub-populations were identified in sheep, including CD105+SSEA-4+c-kit+Isl1+GATA-4+cells, CD105+SSEA-4+c-kit+Isl1+GATA-4-cells, CD105+SSEA-4-c-kit-Isl1+GATA-4-cells, and CD105+SSEA-4-c-kit+Isl1+GATA-4-cells. Immunohistochemical staining for SSEA-4 showed that labeled cells were most abundant in the right atrium of fetal hearts where niches of progenitor cells could be identified. Conclusion:We determined the phenotype and distribution of cardiac progenitor cells in the sheep heart. The availability of cloned endogenous cardiac progenitor cells from sheep will provide a valuable resource for optimizing the conditions for cardiac repair in the ovine model.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 54%

Isolation, Characterization, and Spatial Distribution of Cardiac Progenitor Cells in the Sheep Heart

Hou

Appleby²,

Fuentes³

et al. 2013

J Clin Exp Cardiolog

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show abstract

“…Some reports have suggested that the main source of CSCs is the atrium, 13) especially the RA appendage; 9,12) however, another study demonstrated the opposite result. 25) When we compared the samples derived from the same patient, we observed that the number of c-kit pos cells was significantly higher in the RA than in LA. In addition, the number of cells from the ventricular tissue was the lowest.…”

Section: Discussionmentioning

confidence: 94%

Factors for C-Kit Expression in Cardiac Outgrowth Cells and Human Heart Tissue

et al. 2017

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“…In other words, an apparently fixed population of CPC resides in the heart postnatally. Pouly (Pouly et al, 2008) reported that CPC concentration in the right atrium is greater than that of the septum of transplanted hearts. Other myocardial niches have been reported.…”

Section: Postnatal Distribution Of Cpc In the Heartmentioning

confidence: 99%