Cardiac repair with injectable cell sheet fragments of human amniotic fluid stem cells in an immune-suppressed rat model

Yeh, Yi-Chun; Lee, Wen-Yu; Yu, Chu-Leng; Hwang, Shiaw-Min; Chung, Min‐Fan; Hsu, Li-Wen; Chang, Yen; Lin, Wei-Wen; Tsai, Ming‐Song; Wei, Hao-Ji; Sung, Hsing‐Wen

doi:10.1016/j.biomaterials.2010.04.069

Cited by 73 publications

(48 citation statements)

References 36 publications

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“…49,54 One week after acute MI, saline, dissociated AFSC, or aggregates of AFSC in the form of fragmented cell sheets or cell bodies were injected into the border of the infarct zone. 49,54 Both forms of AFSC aggregates were compared to saline and dissociated AFSC, but not to the other type of aggregate.…”

Section: Injection Of Aggregated Afsc-extracellular Matrix Structuresmentioning

confidence: 99%

“…At room temperature the hydrogel system liquefied and the cell sheet fragments were collected. 54 To form AFSC aggregates, the cells were plated into a 96-well plate coated with methylcellulose hydrogel. Cells do not attach to the surface of the methylcellulose hydrogel and instead aggregate to form spherical cell bodies.…”

Section: Injection Of Aggregated Afsc-extracellular Matrix Structuresmentioning

confidence: 99%

“…18,23,30,35,46,[49][50][51][52]54 Results for expression of c-kit or CD117 in AFSC were mixed: some research demonstrated positive gene expression by PCR, 37 but flow cytometry results were negative 17,49,50 or slightly (*25%) positive. 18 Oct4, Sox2, and Nanog, transcription factors necessary for maintaining pluirpotency in ESCs, also displayed mixed results across different studies of AFSC.…”

mentioning

confidence: 99%

“…18 Oct4, Sox2, and Nanog, transcription factors necessary for maintaining pluirpotency in ESCs, also displayed mixed results across different studies of AFSC. 16,17,20,23,24,[29][30][31]35,37,[39][40][41]46,[48][49][50][51][52]54 In Vitro Cardiac Myocyte Differentiation of AFSC AFSC have been investigated for cardiac tissue engineering. 55 AFSC applications to in vitro cardiac tissue engineering are summarized in this section and Table 3.…”

mentioning

confidence: 99%

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Amniotic Fluid-Derived Stem Cells for Cardiovascular Tissue Engineering Applications

Connell

Camci‐Unal

Khademhosseini

et al. 2013

Tissue Engineering Part B: Reviews

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Recent research has demonstrated that a population of stem cells can be isolated from amniotic fluid removed by amniocentesis that are broadly multipotent and nontumorogenic. These amniotic fluid-derived stem cells (AFSC) could potentially provide an autologous cell source for treatment of congenital defects identified during gestation, particularly cardiovascular defects. In this review, the various methods of isolating, sorting, and culturing AFSC are compared, along with techniques for inducing differentiation into cardiac myocytes and endothelial cells. Although research has not demonstrated complete and high-yield cardiac differentiation, AFSC have been shown to effectively differentiate into endothelial cells and can effectively support cardiac tissue. Additionally, several tissue engineering and regenerative therapeutic approaches for the use of these cells in heart patches, injection after myocardial infarction, heart valves, vascularized scaffolds, and blood vessels are summarized. These applications show great promise in the treatment of congenital cardiovascular defects, and further studies of isolation, culture, and differentiation of AFSC will help to develop their use for tissue engineering, regenerative medicine, and cardiovascular therapies.

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Section: Injection Of Aggregated Afsc-extracellular Matrix Structuresmentioning

confidence: 99%

Section: Injection Of Aggregated Afsc-extracellular Matrix Structuresmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Amniotic Fluid-Derived Stem Cells for Cardiovascular Tissue Engineering Applications

Connell

Camci‐Unal

Khademhosseini

et al. 2013

Tissue Engineering Part B: Reviews

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“…Thus, stem cell technology can be used to model and treat human ailments such as type 1 diabetes, 25 Parkinson's, 26 and cardiovascular disease. 27 Furthermore, stem cells can provide a source of human cells, which can be used in combination with high-throughput technologies for drug screening and toxicology, providing insights that traditional cell lines cannot. 16,28,29 Despite the enthusiasm over stem cells, there are several limitations with stem cell research that have clouded our understanding of the basic science of stem cells and delayed progress to immediate clinical application of stem cells (Table 1).…”

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confidence: 99%

Technology Advancement for Integrative Stem Cell Analyses

Jeong

Choi

Lee

2014

Tissue Engineering Part B: Reviews

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Scientists have endeavored to use stem cells for a variety of applications ranging from basic science research to translational medicine. Population-based characterization of such stem cells, while providing an important foundation to further development, often disregard the heterogeneity inherent among individual constituents within a given population. The population-based analysis and characterization of stem cells and the problems associated with such a blanket approach only underscore the need for the development of new analytical technology. In this article, we review current stem cell analytical technologies, along with the advantages and disadvantages of each, followed by applications of these technologies in the field of stem cells. Furthermore, while recent advances in micro/nano technology have led to a growth in the stem cell analytical field, underlying architectural concepts allow only for a vertical analytical approach, in which different desirable parameters are obtained from multiple individual experiments and there are many technical challenges that limit vertically integrated analytical tools. Therefore, we propose-by introducing a concept of vertical and horizontal approach-that there is the need of adequate methods to the integration of information, such that multiple descriptive parameters from a stem cell can be obtained from a single experiment.

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Injectable Cell Constructs Fabricated via Culture on a Thermoresponsive Methylcellulose Hydrogel System for the Treatment of Ischemic Diseases

Huang

Liao

Chen

et al. 2014

Adv Healthcare Materials

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Cell transplantation via direct intramuscular injection is a promising therapy for patients with ischemic diseases. However, following injections, retention of transplanted cells in engrafted areas remains problematic, and can be deleterious to cell-transplantation therapy. In this Progress Report, a thermoresponsive hydrogel system composed of aqueous methylcellulose (MC) blended with phosphate-buffered saline is constructed to grow cell sheet fragments and cell bodies for the treatment of ischemic diseases. The as-prepared MC hydrogel system undergoes a sol-gel reversible transition upon heating or cooling at ≈32 °C. Via this unique property, the grown cell sheet fragments (cell bodies) can be harvested without using proteolytic enzymes; consequently, their inherent extracellular matrices (ECMs) and integrative adhesive agents remain well preserved. In animal studies using rats and pigs with experimentally created myocardial infarction, the injected cell sheet fragments (cell bodies) become entrapped in the interstices of muscular tissues and adhere to engraftment sites, while a minimal number of cells exist in the group receiving dissociated cells. Moreover, transplantation of cell sheet fragments (cell bodies) significantly increases vascular density, thereby improving the function of an infarcted heart. These experimental results demonstrate that cell sheet fragments (cell bodies) function as a cell-delivery construct by providing a favorable ECM environment to retain transplanted cells locally and consequently, improving the efficacy of therapeutic cell transplantation.

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Cardiac repair with injectable cell sheet fragments of human amniotic fluid stem cells in an immune-suppressed rat model

Cited by 73 publications

References 36 publications

Amniotic Fluid-Derived Stem Cells for Cardiovascular Tissue Engineering Applications

Amniotic Fluid-Derived Stem Cells for Cardiovascular Tissue Engineering Applications

Technology Advancement for Integrative Stem Cell Analyses

Injectable Cell Constructs Fabricated via Culture on a Thermoresponsive Methylcellulose Hydrogel System for the Treatment of Ischemic Diseases

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