Cardiac pre-differentiation of human mesenchymal stem cells by electrostimulation

Genovese, Jorge; Spadaccio, Cristiano; Chachques, Emmanuel; Schussler, Olivier; Carpentier, A; Chachques, Juan Carlos; Patel, Amit N.

doi:10.2741/3429

Cited by 37 publications

(40 citation statements)

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“…Electrical stimulation of mesenchymal stem cells has been shown to induce morphological changes to a striated muscle cell phenotype that expressed cardiac specific markers [44]. Other cell populations, such as fibroblasts, have been shown to display pre-commitment into a cardiomyocyte phenotype with short-time electrical stimulation.…”

Section: Effects Of Electrical Stimulation On Transplanted Cellsmentioning

confidence: 99%

The Role of Cardiac Electrophysiology in Myocardial Regenerative Stem Cell Therapy

Huang

Pashmforoush

Chung

et al. 2010

J. of Cardiovasc. Trans. Res.

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Recent advances in stem cell biology and tissue engineering have put forth new therapeutic paradigms for treatment of myocardial disease. The aim of stem cell therapy for myocardial regeneration has been directed to induce angiogenesis for ischemic heart disease and/or introduction of new cardiomyocytes to improve the mechanical function of the failing heart. Encouraged by positive preliminary results in mouse models of myocardial infarction, clinical trials have utilized autologous skeletal myoblasts and bone-marrow-derived stem cells to treat patients in various clinical settings including acute myocardial injury, chronic angina, and heart failure. These studies have collectively shown, at best, modest improvement in cardiac function. This may be due to the fact that there is little evidence to support actual formation and/or integration of transplanted cells into the recipient myocardium. More recent and emerging data supports the finding that electrical stimulation may be an effective catalyst for sustained functional organization, integration, and maturation of transplanted cell populations into the host myocardium. A therapeutic model that utilizes electrical stimulation and/or achieves cardiac resynchronization in conjunction with stem cell transplantation may be an effective means to achieve successful myocardial regenerative therapy.

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Section: Effects Of Electrical Stimulation On Transplanted Cellsmentioning

confidence: 99%

The Role of Cardiac Electrophysiology in Myocardial Regenerative Stem Cell Therapy

Huang

Pashmforoush

Chung

et al. 2010

J. of Cardiovasc. Trans. Res.

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“…There are other biotechnological advances: matrixes may be improved in the future by crosslinking angiogenic growth factors, absorption delay will be prolonged by nanotechnologies and additional chemical-physical crosslinking, and stem cell preconditioning will be achieved either by hypoxic conditions during culture or by in vitro electrostimulation [14][15][16].…”

Section: Onementioning

confidence: 99%

General Overview of the Sixth International Symposium on Stem Cell Therapy and Cardiovascular Innovations

Vázquez-Álvarez

Sanz‐Ruiz

Gutiérrez

et al. 2009

J. of Cardiovasc. Trans. Res.

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Being one of the main stem cell therapy meetings of the year, the Sixth International Symposium on Stem Cell Therapy and Cardiovascular Innovations was held on April 23rd-24th, 2009, at the Auditorium of the High Council of Scientific Research of Spain (CSIC) in Madrid. Gathering the most prestigious basic researchers and clinical experts in the field of cardiovascular regenerative medicine, the aim of the meeting was to discuss the available evidence and the recent contributions from preclinical investigators, cardiologists, and cardiac surgeons in a participative translational fashion. The role of young "clinician scientists" was reinforced with a special poster session and three awards. The main conclusions of the symposium were (1) that standardization, larger clinical trials, and true translational research are needed, and (2) that new-allogeneic-stem cell products, biotechnological devices, and cell-based bioartificial organs are potentially exciting options for the future.

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“…Apoptosis can be induced by anchorage-dependent cells detaching from the surrounding extracellular matrix. The development of strategies for improving cell survival and differentiation should be encouraged, such as by using tissue engineering, prevascularization, pre conditioning procedures such as in vitro cell electrostimulation, cell cultures under hypoxic conditions and combination of angiogenic and myogenic stem cells [40][41][42][43].…”

Section: Stem Cell Niche and Cell Homingmentioning

confidence: 99%

Cellular cardiac regenerative therapy in which patients?

Chachques¹

2009

Expert Review of Cardiovascular Therapy

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Cell-based myocardial regenerative therapy is undergoing experimental and clinical trials in order to limit the consequences of decreased contractile function and compliance of damaged ventricles owing to ischemic and nonischemic myocardial diseases. A variety of myogenic and angiogenic cell types have been proposed, such as skeletal myoblasts, mononuclear and mesenchymal bone marrow cells, circulating blood-derived progenitors, adipose-derived stromal cells, induced pluripotent stem cells, umbilical cord cells, endometrial mesenchymal stem cells, adult testis pluripotent stem cells and embryonic cells. Current indications for stem cell therapy concern patients who have had a left- or right-ventricular infarction or idiopathic dilated cardiomyopathies. Other indications and potential applications include patients with diabetic cardiomyopathy, Chagas heart disease (American trypanosomiasis), ischemic mitral regurgitation, left ventricular noncompacted myocardium and pediatric cardiomyopathy. Suitable sources of cells for cardiac implant will depend on the types of diseases to be treated. For acute myocardial infarction, a cell that reduces myocardial necrosis and augments vascular blood flow will be desirable. For heart failure, cells that replace or promote myogenesis, reverse apoptopic mechanisms and reactivate dormant cell processes will be useful. It is important to note that stem cells are not an alternative to heart transplantation; selected patients should be in an early stage of heart failure as the goal of this regenerative approach is to avoid or delay organ transplantation. Since the cell niche provides crucial support needed for stem cell maintenance, the most interesting and realistic perspectives include the association of intramyocardial cell transplantation with tissue-engineered scaffolds and multisite cardiac pacing in order to transform a passive regenerative approach into a 'dynamic cellular support', a promising method for the creation of 'bioartificial myocardium'.

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Cardiac pre-differentiation of human mesenchymal stem cells by electrostimulation

Cited by 37 publications

References 0 publications

The Role of Cardiac Electrophysiology in Myocardial Regenerative Stem Cell Therapy

The Role of Cardiac Electrophysiology in Myocardial Regenerative Stem Cell Therapy

General Overview of the Sixth International Symposium on Stem Cell Therapy and Cardiovascular Innovations

Cellular cardiac regenerative therapy in which patients?

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