Cardiac neural crest cells contribute to the dormant multipotent stem cell in the mammalian heart

Tomita, Yuichi; Matsumura, Keisuke; Wakamatsu, Yoshio; Matsuzaki, Yuji; Shibuya, Isao; Kawaguchi, Haruko; Ieda, Masaki; Kanakubo, Sachiko; Shimazaki, Takuya; Ogawa, Satoshi; Osumi, Noriko; Okano, Hideyuki; Fukuda, Keiichi

doi:10.1083/jcb.200504061

Cited by 300 publications

(285 citation statements)

References 52 publications

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“…This discrepancy in expression levels suggests that nestin + and GATA-4 + cells could be derived from other cell lineages, including c-Kit -cells. In this respect, Tomita et al (2005) showed that neural crest-derived cells migrate and lay in a dormant, undifferentiated state in the heart expressing nestin and GATA-4 or can differentiate into cardiomyocytes or in vitro-derived cardiospheres.…”

Section: Discussionmentioning

confidence: 99%

Research of cardiomyocyte precursors in adult rat heart

Bellafiore

Cappello

et al. 2006

Tissue and Cell

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Recent reports supported the existence of stem cells in adult hearts. However, phenotype and localization of these cells have not been completely described and it is unknown if cardiac regenerative potential differs from one subject to another. The aims of our work were to identify different populations of cardiac stem cells by the analysis of specific markers and to evaluate the expression variability of these markers in 12 adult rat hearts. The expression of CD9, taube nuss and nanog suggests the presence of stem cells from the earliest stages of embryogenesis in adult myocardium. Their different expression could be associated to the degree of stem cell differentiation. CD34 and c-Kit antibodies were used to detect stem cells committed to one or more specific tissue lineages and we found a strong immunoreactivity for CD34 exclusively in the endothelial cells and a low positivity for c-Kit in the interstitium and next to the vessels. Moreover, as c-Kit expression highly differed within all examined hearts, we suggest that cardiomyogenic potential is different among the various subjects. Undifferentiated cells with myogenic-committed phenotype expressing GATA-4 and nestin were found, respectively, in the interstitial and myocardial cells and in few interstitial cells. Therefore, the physiologic turn over of cardiomyocytes may occur in adult hearts as it has been shown in many others organs. The study of myogenic potential could be important to identify markers specific of stem cells in in vivo adult myocardium that may be used to purify these cells and evaluate their regenerative ability.

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Section: Discussionmentioning

confidence: 99%

Research of cardiomyocyte precursors in adult rat heart

Bellafiore

Cappello

et al. 2006

Tissue and Cell

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“…Yet, endogenous Gata-4 has not been detected (Pilon et al, 2008). In other reports, Gata-4 expression has been described in some neural crest-derived cardiac progenitors (Tomita et al, 2005) and in primitive neural stem cell spheres (Hitoshi et al, 2004).…”

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confidence: 97%

GATA factors in human neuroblastoma: distinctive expression patterns in clinical subtypes

et al. 2009

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BACKGROUND: The aim of this study is to elucidate the expression patterns of GATA transcription factors in neuroblastoma and the developing sympathetic nervous system (SNS). METHODS: GATA-2, -3 and -4 and their cofactor friend-of-GATA (FOG)-2 were investigated in primary neuroblastoma by immunohistochemistry, real-time RT-PCR (n ¼ 73) and microarray analysis (n ¼ 251). In addition, GATA-2, -3 and FOG-2 expression was determined by northern-blot hybridisation. In the developing murine SNS, Gata-4 and Fog-2 were examined by immunohistochemistry. RESULTS: Although Gata-2, -3 and Fog-2 are expressed in the developing nervous system, Gata-4 was not detected. In contrast, protein expression of all factors was observed in human neuroblastoma. Northern-blot hybridisation and real-time RT-PCR suggested specific expression patterns of the four genes in primary neuroblastoma, but did not show unequivocal results. In the large cohort examined by microarrays, a significant association of GATA-2, -3 and FOG-2 expression with low-risk features was observed, whereas GATA-4 mRNA levels correlated with MYCN-amplification. CONCLUSION: The transcription factors GATA-2 and -3, which are essential for normal SNS development, and their cofactor FOG-2 are downregulated in aggressive but not in favourable neuroblastoma. In contrast, upregulation of GATA-4 appears to be a common feature of this malignancy and might contribute to neuroblastoma pathogenesis.

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“…When transplanted into chick neural tube, cardiosphere cells behaved as neural crest and migrated into the outflow tract, DRGs and spinal nerve and followed the lateral migration pathway. Together, this evidence suggests that a population of neural crest-derived stem cells remains in the heart and has capacity to differentiate (Tomita et al, 2005).…”

Section: Cardiac Ncscsmentioning

confidence: 83%

“…A neural crest-derived stem cell population has also been identified within the adult mice cardiac side population (SP; Tomita et al, 2005). SP cells are dormant, multipotent cells that are found in a variety of tissues and can be identified by their differential ability to efflux the Hoechst 33342 dye.…”

Section: Cardiac Ncscsmentioning

confidence: 99%

The proliferating field of neural crest stem cells

Delfino‐Machín

Chipperfield

Rodrigues

et al. 2007

Developmental Dynamics

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Neural crest stem cells were first isolated from early embryonic neural crest in the early 1990s, but in the past 5 years, there has been a burst of discoveries of neural crest-derived stem cells from diverse locations. Here, we summarize these data, highlighting the characteristics of each stem cell type. These cells vary widely in the markers they express and the variety of cell types they appear to generate. They occupy diverse locations, but in some cases multiple stem cell types apparently occupy physically proximate niches. To date, few molecular similarities can be identified between these stem cells, although a systematic comparison is required. We note other issues worthy of attention, including aspects of the in vivo behavior of these stem cells, their niches, and their lineage relationships. Together, analysis of these issues will clarify this expanding, but still young, field and contribute to exploration of the important therapeutic potential of these cells. Developmental Dynamics 236:3242-3254, 2007.

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Cardiac neural crest cells contribute to the dormant multipotent stem cell in the mammalian heart

Cited by 300 publications

References 52 publications

Research of cardiomyocyte precursors in adult rat heart

Research of cardiomyocyte precursors in adult rat heart

GATA factors in human neuroblastoma: distinctive expression patterns in clinical subtypes

The proliferating field of neural crest stem cells

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