Cardiac Endothelial Heterogeneity Defines Valvular Development as Demonstrated by the Diverse Expression of JB3, an Antigen of the Endocardial Cushion Tissue

Wunsch, Ann M.; Little, Charles D.; Markwald, Roger R.

doi:10.1006/dbio.1994.1278

Cited by 120 publications

(81 citation statements)

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“…It was recently shown that elastin is expressed much earlier than previously thought (Visconti et al, 2003), but it is still far behind the initial expression of fibrillin-1 and -2 (Gallagher et al, 1993;Wunsch et al, 1994) and FB1 antigen (Fig. 2).…”

Section: Discussionmentioning

confidence: 81%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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In this study, a unique fiber system in the subectodermal mesenchyme of the chick limb bud was visualized immunohistochemically with the use of a novel monoclonal antibody termed "FB1." This antibody stained a subset of extracellular fibers in the embryonic mesenchyme. Among the fibers visualized, those running perpendicularly to the limb bud ectoderm became progressively prominent in their thickness and length, and organized into a parallel array in the subectodermal region. This fiber system was distinct from that of major collagens, fibronectin, or tenascin. A molecule immunoprecipitated with FB1 comigrated with JB3 antigen, or chicken fibrillin-2. The fibers visualized immunohistochemically by FB1 and JB3 were indistinguishable from each other, and ultrastructurally appeared to be bundles composed of tubular-like microfibrils that originated directly from the ectodermal basal lamina. They lacked the amorphous deposits that are characteristic of elastin. A similar array of subectodermal fibers was also found in the developing axilla and some truncal regions, again well before the development of a definitive dermis. These findings suggest that a parallel array of subectodermal FB1-positive fibers constitutes a precocious fiber system in the presumptive dermis prior to the substantial formation of collagenous fibers. These fibers could be developmentally linked to oxytalan fibers, which are known to be present in the papillary dermis in mature cutaneous tissue.

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Section: Discussionmentioning

confidence: 81%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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“…Within the developing atrioventricular (AV) canal, TGF␤ stimulates transformation of endothelial cells to mesenchymal cells, which will form the AV valves and septa (Wunsch et al, 1994). Defects of the AV valves and septa are among the most commonly diagnosed neonatal heart malformations and are thought to result from abnormal interactions between mesenchymal cushion cells and the ECM (Srivastava, 2001).…”

Section: Discussionmentioning

confidence: 99%

Hyperglycemia‐induced TGFβ and fibronectin expression in embryonic mouse heart

Smoak

2004

Developmental Dynamics

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Cardiovascular defects are common in diabetic offspring, but their etiology and pathogenesis are poorly understood. Extracellular matrix accumulates in adult tissues in response to hyperglycemia, and transforming growth factor-beta1 (TGF␤1) likely mediates this effect. The objective of this study was to characterize TGF␤ expression in the organogenesisstage mouse heart and to evaluate TGF␤ and fibronectin expression in embryonic mouse heart exposed to hyperglycemia. Prominent TGF␤1, and minimal TGF␤2 or TGF␤3, protein expression was demonstrated in embryonic day (E) 9.5-E13.5 hearts. Hyperglycemia for 24 hr produced significantly increased fibronectin, slightly increased TGF␤1, and unchanged TGF␤2 or TGF␤3, by immunohistochemistry. Increased TGF␤1 was demonstrated by enzyme-linked immunosorbent assay in embryonic fluid and isolated hearts after hyperglycemia for 24 hr, but not 48 hr. Hyperglycemia increased fibronectin protein and mRNA expression in embryonic hearts after 24 hr, and pericardial injection of TGF␤1 also increased fibronectin mRNA in the embryonic heart. It is proposed that TGF␤1 and fibronectin may play a role in diabetes-induced cardiac dysmorphogenesis.

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“…Fibrillin was detected with the monoclonal antibody [JB3] (Wunsch et al, 1994), which recognizes chick fibrillin 2 (Rongish et al, 1998) and was provided by Charlie Little. Western blotting was performed on whole embryo extracts run on 5% acryamide gels, electroblotted onto nitrocellulose and developed with the JB3 antibody and HRP conjugated secondary antibody and supersignal detection reagents (Pierce, Rockford, IL).…”

Section: Embryo Manipulations and Immunohistochemistrymentioning

confidence: 99%

“…The embryonic pattern of deposition of the fibrillin proteins has been determined at the highest resolution in chicken, where Fbn 1 is first found at gastrulation both as fibrils radiating from Hensen's node, the avian equivalent of the frog organizer, as well as along the primitive groove or primary axis prior to al., 2000). Chicken Fbn 2 is also first found in axial structures during somitogenesis, surrounding the notochord and surrounding each somite as it segments from the prechordal plate (Wunsch et al, 1994;Rongish et al, 1998;Czirok et al, 2004). Fbn 3 is also present in embryos, but its distribution is less well characterized (Corson et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Fbn 3 is also present in embryos, but its distribution is less well characterized (Corson et al, 2004). In the chicken dorsal axis, a mechanical role for the fibrillins in maintaining tissue integrity has been postulated, both prior to notochord development and at later stages as a fibrillar, basket-like network holding axial embryonic elements such as notochord, somites, dorsal aorta, and gut in relative position (Gallagher et al, 1993;Wunsch et al, 1994;Rongish et al, 1998;Czirok et al, 2004). The dynamic behavior of Fbn 2 fibrils during axis formation in the chicken embryo reveals strong cell-matrix interactions, because fibril displacement in the axis is similar to the pattern of cell movement described in the gastrula (Czirok et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Xenopus fibrillin is expressed in the organizer and is the earliest component of matrix at the developing notochord‐somite boundary

Skoglund

Dzamba

Coffman

et al. 2006

Developmental Dynamics

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We identify a Xenopus fibrillin homolog (XF), and show that its earliest developmental expression is in presumptive dorsal mesoderm at gastrulation, and that XF expression is regulated by mesoderm-inducing factors in animal cap assays. XF protein is also first detected in presumptive mesoderm, but is concentrated specifically into extracellular-matrix structures that begin to develop de novo by mid-gastrulation at both of the bilateral presumptive notochord-somite boundaries. Later in embryogenesis, XF protein is localized to the extracellular matrix at tissue boundaries, where it is found surrounding the notochord, the somites, and the neural tube, as well as under the epidermis. This pattern of protein deposition combines to give the appearance of an "embryonic skeleton," suggesting that one role for XF is to serve as a mechanical element in the embryo prior to bone deposition.

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Cardiac Endothelial Heterogeneity Defines Valvular Development as Demonstrated by the Diverse Expression of JB3, an Antigen of the Endocardial Cushion Tissue

Cited by 120 publications

References 0 publications

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

Hyperglycemia‐induced TGFβ and fibronectin expression in embryonic mouse heart

Xenopus fibrillin is expressed in the organizer and is the earliest component of matrix at the developing notochord‐somite boundary

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