Cardamonin inhibits the growth of human osteosarcoma cells through activating P38 and JNK signaling pathway

Zhang, Lulu; Yang, Chunmei; Huang, Yanran; Huang, Huakun; Yuan, Xiaohui; Zhang, Ping; Ye, Caihong; Wei, Mengqi; Wang, Yuping; Luo, Xiaoji; Luo, Jinyong

doi:10.1016/j.biopha.2020.111155

Cited by 19 publications

(15 citation statements)

References 45 publications

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“…Because of controversial role of Erk1/2 in cell proliferation and cell death [ 81 ], additional studies are needed to elucidate the function of this kinase in these processes. In addition, we also studied phosphorylation status of p38 MAPK due to fact that activation of this kinase is accompanied with apoptosis induction [ 82 , 83 , 84 ]. Our experiments have shown that chalcone ZK-CH-11d-induced an increase in p38 MAPK phosphorylation in both cancer lines.…”

Section: Discussionmentioning

confidence: 99%

Programmed Cell Death Alterations Mediated by Synthetic Indole Chalcone Resulted in Cell Cycle Arrest, DNA Damage, Apoptosis and Signaling Pathway Modulations in Breast Cancer Model

et al. 2022

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Although new chemotherapy significantly increased the survival of breast cancer (BC) patients, the use of these drugs is often associated with serious toxicity. The discovery of novel anticancer agents for BC therapy is expected. This study was conducted to explore the antiproliferative effect of newly synthesized indole chalcone derivative ZK-CH-11d on human BC cell lines. MTT screening, flow cytometry, Western blot, and fluorescence microscopy were used to evaluate the mode of cell death. ZK-CH-11d significantly suppressed the proliferation of BC cells with minimal effect against non-cancer cells. This effect was associated with cell cycle arrest at the G2/M phase and apoptosis induction. Apoptosis was associated with cytochrome c release, increased activity of caspase 3 and caspase 7, PARP cleavage, reduced mitochondrial membrane potential, and activation of the DNA damage response system. Furthermore, our study demonstrated that ZK-CH-11d increased the AMPK phosphorylation with simultaneous inhibition of the PI3K/Akt/mTOR pathway indicating autophagy initiation. However, chloroquine, an autophagy inhibitor, significantly potentiated the cytotoxic effect of ZK-CH-11d in MDA-MB-231 cells indicating that autophagy is not principally involved in the antiproliferative effect of ZK-CH-11d. Taking together the results from our experiments, we assume that autophagy was activated as a defense mechanism in treated cells trying to escape from chalcone-induced harmful effects.

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Section: Discussionmentioning

confidence: 99%

Programmed Cell Death Alterations Mediated by Synthetic Indole Chalcone Resulted in Cell Cycle Arrest, DNA Damage, Apoptosis and Signaling Pathway Modulations in Breast Cancer Model

et al. 2022

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“…We then performed enrichment analysis using Metascape. Genes involving in the tan module were mainly enriched in osteosarcoma-related biological processes including "regulation of peptidase activity" [16][17][18], "phenylalanine metabolism" [19,20], and "regulation of growth" [21,22]. Also, genes involving in the midnight blue module were mainly enriched in osteosarcoma-related biological processes, including "DNA damage/ telomere stress-induced senescence" [23][24][25], "metalloprotease DUBs" [26][27][28], and "interferon alpha/beta signaling" [29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Identification of LTF as a Prognostic Biomarker for Osteosarcoma

Liu

Wang

Liu

et al. 2022

Journal of Oncology

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Osteosarcoma remains a major health problem in teenagers. However, its pathogenesis mechanism remains not fully elucidated. This study aims to identify the prognostic biomarkers for osteosarcoma. In this study, we selected genes with a median absolute deviation (MAD) value of the top 5000 in the GSE32981 dataset for subsequent analysis. Weighted correlation network analysis (WGCNA) was used to construct a coexpression network. WGCNA showed that the tan module and midnight blue module were highly correlated with origin and metastases of osteosarcoma, respectively. Enrichment analysis was conducted using genes in the tan module and midnight blue module. A gene coexpression network was constructed by calculating the Spearman correlation coefficients. Four key genes (LTF, C10orf107, HIST1H2AK, and NEXN) were identified to be correlated with the prognosis of osteosarcoma patients. LTF has the highest AUC value, and its effect on osteosarcoma cells was then evaluated. The effect of LTF overexpression on proliferation, migration, and invasion of MG63 and 143B cells was detected by the CCK-8 assay, transwell cell migration assay, and transwell invasion assay, respectively. The overexpression of LTF promoted the proliferation, migration, and invasion of MG63 and 143B cells. In conclusion, LTF may serve as a prognostic biomarker for osteosarcoma.

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“…Western blot analyses showed increased expression of proapoptotic Bax and BAD proteins and down regulation of antiapoptotic Bcl-2 protein. Moreover, activation of caspase-3 and PARP cleavage has also been observed [ 69 ].…”

Section: Induction Of Cell Deathmentioning

confidence: 99%

Molecular Mechanisms of Antiproliferative Effects of Natural Chalcones

Michalková¹,

Mirossay²,

Gazdova³

et al. 2021

Cancers

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Although great progress has been made in the treatment of cancer, the search for new promising molecules with antitumor activity is still one of the greatest challenges in the fight against cancer due to the increasing number of new cases each year. Chalcones (1,3-diphenyl-2-propen-1-one), the precursors of flavonoid synthesis in higher plants, possess a wide spectrum of biological activities including antimicrobial, anti-inflammatory, antioxidant, and anticancer. A plethora of molecular mechanisms of action have been documented, including induction of apoptosis, autophagy, or other types of cell death, cell cycle changes, and modulation of several signaling pathways associated with cell survival or death. In addition, blockade of several steps of angiogenesis and proteasome inhibition has also been documented. This review summarizes the basic molecular mechanisms related to the antiproliferative effects of chalcones, focusing on research articles from the years January 2015–February 2021.

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Cardamonin inhibits the growth of human osteosarcoma cells through activating P38 and JNK signaling pathway

Cited by 19 publications

References 45 publications

Programmed Cell Death Alterations Mediated by Synthetic Indole Chalcone Resulted in Cell Cycle Arrest, DNA Damage, Apoptosis and Signaling Pathway Modulations in Breast Cancer Model

Programmed Cell Death Alterations Mediated by Synthetic Indole Chalcone Resulted in Cell Cycle Arrest, DNA Damage, Apoptosis and Signaling Pathway Modulations in Breast Cancer Model

Identification of LTF as a Prognostic Biomarker for Osteosarcoma

Molecular Mechanisms of Antiproliferative Effects of Natural Chalcones

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