CARD10 promotes the progression of renal cell carcinoma by regulating the NF‑κB signaling pathway

Abstract

Previous studies have demonstrated that the expression of CARD10 is closely associated with the occurrence of tumors, and its role is mainly to promote tumor progression by activating the transcription factor NF-κB. However, the signaling pathway in renal cancer remains unclear. The objective of the present study was to investigate the ability of caspase recruitment domain 10 (CARD10) to regulate the NF-κB signaling pathway and promote the progression of renal cell carcinoma (RCC). Expression of CARD10 in ACHN, 786-O and HK-2 cells was evaluated via western blot analysis, as was the epidermal growth factor (EGF)-induced activation of NF-κB signaling pathway-related proteins in cells. The expression of CARD10 was inhibited by CARD10 short hairpin RNA transfection. Cell cycle analysis and MTT assays were used to evaluate cell proliferation. Cell apoptosis was analyzed via flow cytometry. The invasion of renal cell lines was detected via Transwell cell migration and invasion assays in vitro. The results showed that CARD10 expression was significantly higher in RCC cells than in normal renal tubular epithelial cells. CARD10 silencing inhibited the proliferation, invasion and migration of RCC cells. EGF stimulation upregulated the activation of the NF-κB pathway in RCC cells. Inhibition of CARD10 expression inhibited NF-κB activation in RCC cells. Taken together, these data suggested that CARD10 promotes the progression of renal cell carcinoma by regulating the NF-κB signaling pathway. Thus, this indicated that CARD10 may be a novel therapeutic target in RCC.

CARD14 Signalling Ensures Cell Survival and Cancer Associated Gene Expression in Prostate Cancer Cells

“…To this end, we explored The Cancer Genome Atlas (TCGA) database and analysed Disease/progression-free survival of PCa patients expressing high or low levels of CARD14 , BCL10 , and MALT1 . Because the CARD14-related protein CARD10 was previously reported to contribute to other epithelial carcinomas [ 21 , 22 , 23 , 24 ], we also analysed its expression in PCa. Patients with high CARD14 expression were found to have a significantly lower Disease/progression-free survival compared with patients with low CARD14 expression ( Figure 1 A).…”

“…MALT1 knockdown reduces proliferation, invasion, and migration in PCa cell lines, as well as attenuates xenograft tumour establishment in nude mice [ 19 , 20 ]. Although CARD11 and CARD10 have been previously shown to contribute to lymphoid malignancies and epithelial carcinomas, respectively [ 21 , 22 , 23 , 24 ], CARD14 signalling has so far been mainly studied in keratinocytes in the context of psoriasis [ 6 ]. However, elevated CARD14 expression is also observed in human epithelial cancers [ 25 ].…”

Mechanobiological responses of astrocytes in optic nerve head due to biaxial stretch

“…ANTXR2 is a membrane-bound molecule that can interact with LRP6. LRP6 is an important surface receptor in the Wnt/β–catenin pathway, and thus can affect osteoblastic activity [ 36 ]. In String database ( https://string-db.org ), we found that ANTXR2 and LRP6 had a co-expression relationship (Supplementary 2 ).…”

Proteasome Inhibitors Interrupt the Activation of Non-Canonical NF-κB Signaling Pathway and Induce Cell Apoptosis in Cytarabine-Resistant HL60 Cells

“…LCK activates NF-κB signaling through tyrosine phosphorylation of IκBα [ 39 , 40 ]. CARD10 is closely associated with tumor development because of its role in promoting tumor progression by activating NF-κB [ 41 , 42 ]. However, CARD10 has also been identified as a novel target of CEBPE, an essential transcription factor required for granulocytic differentiation [ 43 ].…”