Carcinomas of the Thyroid with Ewing Family Tumor Elements (CEFTEs): A Diagnostic Challenge Before Surgery

Taccogna, Silvia; Guglielmi, Rinaldo; Persichetti, Agnese; Morano, Carmelo; Angelini, Francesco; Ienzi, Sara; Scarpino, Stefania; Liverani, Andrea; Annovazzi, Alessio; Papini, Enrico

doi:10.1007/s12105-020-01145-z

Cited by 8 publications

(11 citation statements)

References 12 publications

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“…CytoMatrix-FFPE block from patient number 4, stained with H&E, clearly showed the presence of papillary clusters, small vessels and a fibrous-connective axis ( Figure 2 B); in this case, the property of CytoMatrix allowed to capture and store cells and also micro-cellular aggregates in its three-dimensional structure, helping the diagnosis of HGUC. CytoFoam is a 12-mm disc previously described in literature and used to adsorb various cytological samples, as fine needle aspiration of thyroid carcinomas or plasma for the evaluation of circulating tumor cells [ 27 , 28 ]. It is a diagnostic tool similar to CytoMatrix, since it is processed as a tissue fragment, formalin-fixed, paraffin-embedded, and used for IHC or other molecular analyses.…”

Section: Resultsmentioning

confidence: 99%

“…It is a diagnostic tool similar to CytoMatrix, since it is processed as a tissue fragment, formalin-fixed, paraffin-embedded, and used for IHC or other molecular analyses. We used CytoFoam, as previously described, to absorb our cell suspensions from urine specimens in order to compare the results obtained with CytoMatrix [ 27 , 28 ]. Interestingly, when compared with this CytoFoam adsorbent support, CytoMatrix can improve the amount of cells and can also confer an architecture or a "tissue-like" structure ( Figure 2 C).…”

Section: Resultsmentioning

confidence: 99%

“…CytoFoam is a 12-mm disc previously described in literature and used to adsorb various cytological samples, as fine needle aspiration of thyroid carcinomas or plasma for the evaluation of circulating tumor cells [ 27 , 28 ]. It is a diagnostic tool similar to CytoMatrix, since it is processed as a tissue fragment, formalin-fixed, paraffin-embedded, and used for IHC or other molecular analyses.…”

Section: Resultsmentioning

confidence: 99%

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Improvement of the Collection, Maintenance, and Analysis of Neoplastic Cells from Urine Specimens with the Use of CytoMatrix

Minasi

Bosco

Moretti

et al. 2021

MPs

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Urine cytology is a non-invasive test used in combination with cystoscopy for screening and follow-up of urothelial carcinoma (UC). Although cytology can be used to efficiently identify high-grade UC, it has a lower accuracy for the diagnosis of low-grade UC or patients with presence of atypical urothelial cells (AUC). For these reasons, ancillary tests have been added to urine cytology in order to improve the accuracy. However, the poor abundance of neoplastic cells in most samples and the absence of a “tissue-like” structure remains a major challenge. We used a novel synthetic support called CytoMatrix which has the property of capturing and storing cells and micro-macro aggregates within its three-dimensional structure. The urine specimens were obtained from 12 patients: 6 with suspected urothelial neoplasia (low- and high-grade) and 6 with AUC or non-neoplastic samples. The first step is the urine samples preparation, through several centrifugation passages; the second step consists in absorbing cells on the CytoMatrix, and in the subsequent formalin fixation, standard processing and paraffin embedding to prepare FFPE-CytoMatrix block. In the final step, sections are consecutively cut, stained with hematoxylin-eosin (H&E), and analyzed via UroVysion FISH and immunohistochemistry (IHC). Using our simple and reliable protocol, we can improve the quality of urine specimens, allowing a better collection, maintenance, and analysis of cells, with the advantage of using ancillary tests to support cytological diagnosis and the advantage of storing cellular material in a FFPE-CytoMatrix block.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Improvement of the Collection, Maintenance, and Analysis of Neoplastic Cells from Urine Specimens with the Use of CytoMatrix

Minasi

Bosco

Moretti

et al. 2021

MPs

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“…Data on preoperative cytological features of CEFTE are scant. In one reported case, the FNAB resulted in a Bethesda V cytology consistent with undifferentiated thyroid carcinoma [133].…”

Section: Carcinoma Of the Thyroid With Ewing Family Tumor Elements (Cmentioning

confidence: 93%

Pitfalls in Challenging Thyroid Tumors: Emphasis on Differential Diagnosis and Ancillary Biomarkers

2020

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Thyroid pathology encompasses a heterogenous group of clinicopathological entities including rare and diagnostically challenging neoplasms. The review is focused on morphological, immunohistochemical, and molecular features of rare thyroid neoplasms that can pose diagnostic problems. The tumors are organized based on growth patterns including thyroid neoplasms with predominantly papillary, follicular, solid, and spindle cell growth pattern, as well as neoplasms with distinct cytological characteristics. A special section is also dedicated to rare thyroid tumors with peculiar patterns including thyroid carcinoma with Ewing family tumor elements and intrathyroidal thymic-related neoplasms.

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“…Extraosseous Ewing sarcoma (EES) is less frequent and can occur at a wide variety of anatomic [3]. Several recent reports have described an adamantinoma-like Ewing sarcoma (ALES) arising in the thyroid gland, based on the identification of EWSR1 rearrangement in neoplasms showing the classic ES morphology with epithelial differentiation (keratin and p63 or p40 expression) [4][5][6][7][8], and carcinoma of the thyroid with Ewing family tumor elements (CEFTEs) when associated with papillary thyroid carcinoma [9][10][11][12]. Here we present the case of a patient diagnosed with EES located in the left thyroid lobe and a review of the current literature.…”

Section: Introductionmentioning

confidence: 99%

Primary Extra-Osseous Ewing Sarcoma of the Thyroid: A Case Report and Review of the Literature

Seipel

Mechahougui

Mach

et al. 2021

Head and Neck Pathol

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Extra-osseous Ewing sarcoma (ES) is a rare and aggressive malignant tumor found in a variety of organs. Primary ES of the thyroid is exceedingly rare and few cases have been documented to date. We describe the case of a 54-year old woman with a history of breast carcinoma in whom a unique hypermetabolic left thyroid nodule was identified during a follow-up PET-CT scan. An ultrasound examination showed a hypoechogenic nodule of 3.7 cm. A cytological diagnosis of poorly differentiated thyroid carcinoma was made, and a total thyroidectomy was performed. The surgical specimen revealed a poorly differentiated neoplasm composed of medium-sized cells with scant cytoplasm, expressing pancytokeratin, CD99 and NKX2.2 but lacking p63 and p40 expression. Molecular analysis revealed a EWSR1-FLI1 fusion transcript supporting the diagnosis of a primary extra-osseous ES of the thyroid. The patient received adjuvant chemotherapy and has no evidence of recurrent disease.

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Carcinomas of the Thyroid with Ewing Family Tumor Elements (CEFTEs): A Diagnostic Challenge Before Surgery

Cited by 8 publications

References 12 publications

Improvement of the Collection, Maintenance, and Analysis of Neoplastic Cells from Urine Specimens with the Use of CytoMatrix

Improvement of the Collection, Maintenance, and Analysis of Neoplastic Cells from Urine Specimens with the Use of CytoMatrix

Pitfalls in Challenging Thyroid Tumors: Emphasis on Differential Diagnosis and Ancillary Biomarkers

Primary Extra-Osseous Ewing Sarcoma of the Thyroid: A Case Report and Review of the Literature

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