Carcinoma cell-specific Mig-7: A new potential marker for circulating and migrating cancer cells

Lindsey, J. Suzanne

doi:10.3892/or.13.1.37

Cited by 16 publications

(38 citation statements)

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“…26 HEC1A, RL95-2 (RL95) endometrial carcinoma, and HT29 colon carcinoma cell lines (all from American Type Culture Collection, Rockville, MD) were cultured as described previously. 6,23 Epidermal growth factor (EGF; Calbiochem, La Jolla, CA) or HGF/SF (R&D Systems, Minneapolis, MN) treatments (each at 20 ng/ml) were incubated for the indicated time with RL95 cells that had been serum-starved for 12 hours. Melanoma cell lines, MUM-2B, MUM-2C, C918, C8161, and A375P, were maintained in RPMI 1640 medium (Life Technologies, Inc., Invitrogen, Grand Island, NY) supplemented with 10% fetal bovine serum (Life Technologies, Inc., Invitrogen), 20 mmol/L HEPES, and 0.1% gentamicin sulfate.…”

Section: Cell Culturesmentioning

confidence: 99%

“…The nude mouse model was used as described previously 23 under Institutional Animal Care and Use Committee approval. Briefly, 1 ϫ 10 5 HEC1A or RL95 viable cells in 250 l of media were combined with 250 l of GF ϩ Matrigel and injected subcutaneously into the dorsal neck region of nu/nu athymic mice.…”

Section: Xenograft Mouse Modelmentioning

confidence: 99%

“…Notably, Mig-7 is not detected in 25 different normal human tissues or in blood from normal patients. 6,23 Signaling that initiates CTBs and tumor cell invasion from RTK c-Met, the hepatocyte growth factor/ scatter factor (HGF/SF) receptor, and ␣v␤5 integrin induces Mig-7 expression. 6,8,24 Based on these data, we hypothesized that Mig-7 expression plays a role in behaviors in common between CTBs and carcinoma cells, namely invasion and vascular cell mimicry.…”

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confidence: 99%

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Overexpression of Carcinoma and Embryonic Cytotrophoblast Cell-Specific Mig-7 Induces Invasion and Vessel-Like Structure Formation

Petty

Garman

Winn

et al. 2007

The American Journal of Pathology

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Molecular requirements for carcinoma cell interactions with the microenvironment are critical for disease progression but are poorly understood. Integrin ␣v␤5, which senses the extracellular matrix, is important for carcinoma cell dissemination in vivo. ␣v␤5 signaling induces Mig-7, a novel human gene product that is apparently carcinoma-specific. We hypothesized that Mig-7 expression facilitates tumor cell dissemination by increasing invasion and vasculogenic mimicry. Results show that embryonic cytotrophoblasts up-regulated Mig-7 expression before they acquired an invasive phenotype capable of pseudovasculogenesis. Mig-7 protein primarily co-localized with vasculogenic mimicry markers factor VIII-associated antigen, vascular endothelial-cadherin, and laminin 5 ␥2 chain domain III fragment in lymph node metastases. Overexpression of Mig-7 increased ␥2 chain domain III fragments known to contain epidermal growth factor (EGF)-like repeats that can activate EGF receptor. Interestingly, EGF also induced Mig-7 expression. Carcinoma cell adhesion to laminins was significantly reduced by Mig-7 expression. Remarkably, in two-dimensional and three-dimensional Matrigel cultures, Mig-7 expression caused invasion and vessel-like structures. Melanoma cells, which were previously characterized to invade aggressively and to undergo vasculogenic mimicry, expressed Mig-7. Taken together, these data suggest that Mig-7 expression allows cells to sense their environment, to invade, and to form vessel-like structures through a novel relationship with laminin 5 ␥2 chain domain III fragments.

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Section: Cell Culturesmentioning

confidence: 99%

Section: Xenograft Mouse Modelmentioning

confidence: 99%

mentioning

confidence: 99%

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Overexpression of Carcinoma and Embryonic Cytotrophoblast Cell-Specific Mig-7 Induces Invasion and Vessel-Like Structure Formation

Petty

Garman

Winn

et al. 2007

The American Journal of Pathology

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“…CCNE and MAL2 have been identified as useful markers in endometrial carcinoma tissue (27). MIG7 was described as a marker gene, which often predicts poor prognosis in patients with endometrial adenocarcinoma (28). CK19 is a marker of epithelial cells and is also used in the alkaline phosphatase-anti-alkaline phosphatase test, which is routinely used in cancer diagnosis (29,30).…”

Section: Introductionmentioning

confidence: 99%

Quantitative PCR marker genes for endometrial adenocarcinoma

Kölbl

Victor

Birk

et al. 2016

Molecular Medicine Reports

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Abstract. Endometrial adenocarcinoma is a common malignancy in women worldwide, with formation of remote metastasis occurring following oncological treatment. Circulating tumor cells (CTCs) are regarded to be the origin of haematogenous metastasis formation. The present study aimed to identify suitable marker genes using a quantitative polymerase chain reaction (qPCR) approach to detect CTCs from blood samples of patients with endometrial carcinoma. Therefore, RNA was isolated from endometrial adenocarcinoma cell lines and from healthy endometrial tissue and reverse transcribed to cDNA, which was then used in qPCR on a number of marker genes. Cytokeratin 19 and claudin 4 were identified as suitable marker genes for CTCs in endometrial adenocarcinoma, due to their high expression in the majority of the cell lines investigated. The expression values of the genes examined varied widely between the different cell lines, which is similar to the variation in the patient samples. Therefore, the necessity for a set of genes for CTC detection and not one single marker gene is demonstrated. qPCR is a fast, cost-efficient and easy to perform technique, which may be used in the detection of CTCs. Investigation of the occurrence of CTCs in cancer patients would aid in the prevention of metastasis and thereby refine treatment.

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“…Induction of MIG-7 mRNA expression is dependent on multiple factors, that include cytokines like epidermal growth factor (EGF) and hepatocyte growth factor (HGF). It has been suggested that MIG-7 could serve as promising marker for circulating cancer cells detection and metastasis [16]. MIG-7 protein induces invasion and vessel-like structure formation by cancer cells in three dimensional (3D) cultures in vitro and the knockdown of MIG-7 gene in cancer cells line causes their reduced invasion in 3D cultures.…”

Section: Introductionmentioning

confidence: 99%

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

Czekierdowski

Czekierdowska

Stachowicz³

et al. 2017

Ginekol Pol

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Objectives:To investigate the possible association of vasculogenic mimicry (VM), VE-cadherin and MIG-7 expression with clinicopathological features of women with malignant ovarian masses. Material and methods:VM was studied with the PAS reaction and VE-cadherin was assessed with immunohistochemistry in 108 women with malignant ovarian tumors. Additionally, quantitative expression of MIG-7 mRNA was performed in 52 ovarian cancers with qRT-PCR.Results: VM was found in 48/108 cases (44%), more often in higher FIGO stage tumors (83% cases; 40 vs. 8; p = 0.01). High expression of VE-cadherin was present in 37% of all ovarian masses. Ovarian tumors without VM more often expressed low levels of VE-cadherin than tumors where VM was found (37.6% vs.14.6%). No expression or very low expression of MIG-7 mRNA was found in all normal ovarian tissues and in 32 cancer samples. Median RQ of MIG-7 mRNA in tumor samples was higher than in normal ovarian tissue (RQ = 0.29 vs. RQ = 0.05, respectively; p < 0.005) and higher than in non-malignant ovarian masses (0.98 vs. 0.05 respectively; p = 0.03). Expression of MIG-7 mRNA was significantly correlated with VM (p = 0.039). In tumors with PAS-positive structures median RQ MIG-7 mRNA was higher than in tumors with PAS-negative findings (1.89 vs. 0.13 respectively). VE-cadherin expression was more frequently found in tumors where MIG-7 mRNA was present (p = 0.004). Conclusions:Vasculogenic mimicry exists in malignant ovarian tumors and advanced clinical stages of malignancy are accompanied by a high incidence of VM formation. MIG-7 mRNA and VE-cadherin expression may serve as additional molecular markers of VM in ovarian malignancies.

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Carcinoma cell-specific Mig-7: A new potential marker for circulating and migrating cancer cells

Cited by 16 publications

References 0 publications

Overexpression of Carcinoma and Embryonic Cytotrophoblast Cell-Specific Mig-7 Induces Invasion and Vessel-Like Structure Formation

Overexpression of Carcinoma and Embryonic Cytotrophoblast Cell-Specific Mig-7 Induces Invasion and Vessel-Like Structure Formation

Quantitative PCR marker genes for endometrial adenocarcinoma

Mig-7 expression and vasculogenic mimicry in malignant ovarian tumors

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