Carcinoembryonic gene member 2 mRNA expression as a marker to detect circulating enterocytes in the blood of colorectal cancer patients

Douard, Richard; Maire, Valérie Le; Wind, Philippe; Sales, Jean-Patrick; Dumas, Frédéric; Fayemendi, Laurent; Landi, Bruno; Benichou, Joseph; Cugnenc, Paul-Henri; Gayral, F; Loric, Sylvain

doi:10.1067/msy.2001.112485

Cited by 15 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mRNA Markers. Table 4 gives an overview of studies (83)(84)(85)(86)(87)(88)(89)(90)(91)(92)(93)(94)(95)(96)(97)(98)(99)(100)(101)(102) applying reverse transcription-PCR to detect mRNA expressed in circulating tumor cells. Free mRNA is not stable in blood, but it can be isolated from circulating cells.…”

Section: Resultsmentioning

confidence: 99%

Blood Markers for Early Detection of Colorectal Cancer: A Systematic Review

Hundt

Haug

Brenner

2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

190

138

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

Blood Markers for Early Detection of Colorectal Cancer: A Systematic Review

Hundt

Haug

Brenner

2007

Cancer Epidemiology, Biomarkers &Amp; Prevention

190

138

View full text Add to dashboard Cite

show abstract

“…Five studies were excluded as they were reviews or systematic reviews, which did not report on prognostic outcomes (Tsavellas et al, 2001;Sleijfer et al, 2007;Riethdorf et al, 2008;Sergeant et al, 2008;Tsouma et al, 2008). Ten studies were excluded as they did not report on prognostic outcomes (Denis et al, 1997;Nakamori et al, 1997;Douard et al, 2001;Guadagni et al, 2001;Patel et al, 2002;Silva et al, 2002;Yokoyama and Yamaue, 2002;Schuster et al, 2004;Yeh et al, 2006;Lagoudianakis et al, 2009). The outcomes measured in the 14 studies included in this review were too heterogeneous with regards to the methodology of data collection to allow quantitative analysis.…”

Section: Resultsmentioning

confidence: 99%

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

et al. 2010

View full text Add to dashboard Cite

Background: The role of adjuvant chemotherapy after resection of colorectal cancers (CRCs) is well understood for patients with stage-I or stage-III disease. Its efficacy for those with stage-II disease remains much less clear. Many investigators have sought to identify prognostic markers that might clarify which patients have the highest risk of recurrence and would, therefore, be most likely to benefit from chemotherapy. This systematic review examines evidence for the use of peripherally sampled, circulating tumour cells (CTCs) as such a prognostic marker. Methods: A comprehensive literature search was used to identify studies reporting on the significance of CTCs in the postoperative blood of CRC patients. Results: Fourteen studies satisfied the inclusion criteria. Six of the nine studies that took blood samples 24 h or more postoperatively found detection of postoperative CTCs to be an independent predictor of cancer recurrence. Conclusion: The presence of CTCs in peripheral blood at least 24 h after resection of CRCs is an independent prognostic marker of recurrence. Further studies are needed to clarify the optimal time point for blood sampling and determine the benefit of chemotherapy in CTC-positive patients with stage-II disease.

show abstract

“…Results were mixed. For example, nine studies spanning the years 1994 to 2002 showed that RT-PCR is a valid and powerful technique for detecting CEA mRNA in peripheral blood, lymph nodes, bone marrow, or even plasma in patients at high risk for metastasis (133)(134)(135)(136)(137)(138)(139)(140)(141), whereas two studies (142,143) showed that RT-PCR is no more sensitive than immunological techniques for detecting micrometastasis. Reasons for these discrepancies have been the lack of standardization of techniques across laboratories, lack of validation, either small number of samples or patients used, use of nested PCR, selection of PCR primers, number of PCR cycles, number of PCR reactions performed for each sample and the interpretation of conflicting PCR results, and the CEA family member shown may be down regulated in colorectal carcinoma.…”

Section: Genes Involved In Metastasismentioning

confidence: 97%

“…This material may not be used or reproduced in any form without the express written permission of Marcel Dekker, Inc. MARCEL DEKKER, INC. • 270 MADISON AVENUE • NEW YORK, NY 10016evaluate the sensitivity and specificity of RT-PCR technology for detecting CEA micrometastasis(143).…”

mentioning

confidence: 99%

Colon Cancer: Prevalence, Screening, Gene Expression and Mutation, and Risk Factors and Assessment

Ahmed

2003

Journal of Environmental Science and Health, Part C

View full text Add to dashboard Cite

Colon cancer detection at an early stage and identifying susceptible individuals can result in reduced mortality from this prevalent cancer. Genetic events leading to the development of this cancer involve a multistage progression of adenoma polyps to invasive metastatic carcinomas. Currently, there is no satisfactory screening method that is highly specific, sensitive, or reliable. Dietary patterns associated with the greatest increase in colon cancer risk are the ones that typify a diet rich in fat and calories, and low in vegetable, fruits, and fibers. Genetic susceptibility to environmental carcinogenesis must be factored into the risk assessment for this cancer. Many genes have been shown to be associated with increased expression and mutations in colorectal cancer patients. These genes have been reviewed; it is hoped that by carefully selecting a number of them, a molecular approach that is suitable for arriving at a tumorigenic expression index is developed, which will reliably detect this cancer at an early stage (i.e., before it metastasizes), especially in exfoliated samples (e.g., stool and blood), so that appropriate intervention strategies can be implemented. Illustrated herein is the utility of employing real-time reverse transcriptase polymerase chain reaction (RT-PCR) to quantitatively measure gene expression, and develop an index that is specific for this cancer, which if perfected may result in a reliable and sensitive screening technique for colorectal cancer detection.

show abstract

Carcinoembryonic gene member 2 mRNA expression as a marker to detect circulating enterocytes in the blood of colorectal cancer patients

Cited by 15 publications

References 21 publications

Blood Markers for Early Detection of Colorectal Cancer: A Systematic Review

Blood Markers for Early Detection of Colorectal Cancer: A Systematic Review

Prognostic significance of circulating tumour cells following surgical resection of colorectal cancers: a systematic review

Colon Cancer: Prevalence, Screening, Gene Expression and Mutation, and Risk Factors and Assessment

Contact Info

Product

Resources

About