Carcinoembryonic antigen family: Characterization of cDNAs coding for NCA and CEA and suggestion of nonrandom sequence variation in their conserved loop-domains

Barnett, Thomas; Goebel, Scott J.; Nothdurft, M.A.; Elting, James J.

doi:10.1016/0888-7543(88)90160-7

Cited by 41 publications

(32 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The exon structure is the same as that reported for other members of the CEA gene family for the immunoglobulin variable-and constant-like domains (33-35, 50, 51) and, likewise, for other members of the immunoglobulin supergene family (58). All of the immunoglobulin-like domain exon sequences reported here are present in the mature CEA mRNA, whose sequence has been determined elsewhere (2,4,36,59 Analysis of the 3' end of the gene has revealed that the hydrophobic membrane domain is encoded by a separate exon (M/3'), which also contains the first 39 nucleotides of the 3' untranslated region ( Fig. 1 and 2).…”

Section: Discussionsupporting

confidence: 52%

“…1 and 2). This region is homologous to a corresponding section of the 3' untranslated region found in NCA cDNA sequences (2,31,47). After this, the sequences of the 3' untranslated regions of the CEA and NCA mRNAs are no longer homologous.…”

Section: Discussionmentioning

confidence: 93%

“…The CEA gene family can be divided into two main subgroups based on sequence comparisons. One subgroup contains the CEA gene itself (2,4,36,59) and those encoding the classic CEA immunocrossreacting antigens, such as the nonspecific cross-reacting antigens (NCA) (2,31,35,47,51) and biliary glycoprotein (3,17). These molecules are membrane bound, either as integral proteins such as biliary glycoprotein or after posttranslational modification through a covalently linked glycosyl phosphatidylinositol moiety as shown for CEA (16,19,45) and an NCA (15,21).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.

Schrewe¹,

Thompson²,

Bona³

et al. 1990

Mol. Cell. Biol.

148

View full text Add to dashboard Cite

Carcinoembryonic antigen (CEA) is a widely used tumor marker, especially in the surveillance of colonic cancer patients. Although CEA is also present in some normal tissues, it is apparently expressed at higher levels in tumorous tissues than in corresponding normal tissues. As a first step toward analyzing the regulation of expression of CEA at the transcriptional level, we have isolated and characterized a cosmid clone (cosCEAl), which contains the entire coding region of the CEA gene. A close correlation exists between the exon and deduced immunoglobulin-like domain borders. We have determined a cluster of transcriptional starts for CEA and the closely related nonspecffic cross-reacting antigen (NCA) gene and have sequenced their putative promoters. Regions of sequence homology are found as far as approximately 500 nucleotides upstream from the translational starts of these genes, but farther upstream they diverge completely. In both cases we were unable to find classic TATA or CAAT boxes at their expected positions. To characterize the CEA and NCA promoters, we carried out transient transfection assays with promoter-indicator gene constructs in the CEA-producing adenocarcinoma cell line SW403, as well as in nonproducing HeLa cells. A CEA gene promoter construct, containing approximately 400 nucleotides upstream from the translational start, showed nine times higher activity in the SW403 than in the HeLa cell line. This indicates that cis-acting sequences which convey cell type-specific expression of the CEA gene are contained within this region.

show abstract

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 93%

mentioning

confidence: 99%

See 1 more Smart Citation

Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.

Schrewe¹,

Thompson²,

Bona³

et al. 1990

Mol. Cell. Biol.

148

View full text Add to dashboard Cite

show abstract

“…The CEA cDNA (gcg:humcea; Ref. 14), kindly provided by Thomas Barnett, was subcloned into the HindIII-XbaI site of pCDM8. Three new constructs, R98A CEA, CBATP-CEA chimera, and CBATP-R98ACEA chimera, were generated using the polymerase chain reaction overlap extension technique (15) and then subcloned into the pCDM8 vector.…”

Section: Methodsmentioning

confidence: 99%

Reconstitution of Bile Acid Transport in a Heterologous Cell by Cotransfection of Transporters for Bile Acid Uptake and Efflux

Sippel¹,

Dawson²,

Shen³

et al. 1997

Journal of Biological Chemistry

View full text Add to dashboard Cite

The rat liver canalicular bile acid transporter/ectoATPase/cell CAM 105 (CBATP) is a 110-kDa transmembrane phosphoglycoprotein that is thought to have bile acid efflux, ecto-ATPase, and cell adhesion properties. Its extracellular amino-terminal domain is highly homologous to carcinoembryonic antigen (CEA), a glycophosphatidyl inositol-anchored membrane protein with cell adhesion properties and a marker for adenocarcinoma. In the current study, we examined the possibility of more clearly defining the role of CBATP in bile acid efflux by cotransfecting a heterologous cell, the COS cell, with cDNAs for a bile acid importer, the ileal bile acid transporter (IBAT), as well as for CBATP. The results show that when IBAT mediates uptake of [

show abstract

“…The members of the CEA gene family are believed to function as adhesion molecules because of evidence such as homology with the neural cell adhesion molecule (N-CAM) and homotypic and heterotypic intercellular adhesion. 21,22 The NCA-50/90 carries the sialyl Lewis-X carbohydrate antigen structure, ligand for the selection family cell-adhesion molecules, and is involved in adherence of granulocytes to activated endothelial cells. 23 The surface expression of NCA-50/90 on leukemic cells also may play a role in cellular survival and tissue infiltration via interactions with BM stromal cells and endothelial or interstitial cells of various tissues.…”

Section: Discussionmentioning

confidence: 99%

True

1999

Leukemia

View full text Add to dashboard Cite

show abstract

Carcinoembryonic antigen family: Characterization of cDNAs coding for NCA and CEA and suggestion of nonrandom sequence variation in their conserved loop-domains

Cited by 41 publications

References 23 publications

Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.

Cloning of the complete gene for carcinoembryonic antigen: analysis of its promoter indicates a region conveying cell type-specific expression.

Reconstitution of Bile Acid Transport in a Heterologous Cell by Cotransfection of Transporters for Bile Acid Uptake and Efflux

True

Contact Info

Product

Resources

About