Carcinoembryonic Antigen and Related Molecules in Normal and Transformed Trophoblast

Vićovac, Ljiljana; Božić, Milica; Bojić‐Trbojević, Žanka; Golubović, Snežana

doi:10.1016/j.placenta.2006.02.002

Cited by 12 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The isolation protocol using sequential trypsin digestion has been reported previously [41], [42]. After isolation, cells were identified as trophoblast by immunocytochemical staining for CK-7, and preparations with >90% cytokeratin positive cell were used for all experiments.…”

Section: Methodsmentioning

confidence: 99%

Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

et al. 2011

View full text Add to dashboard Cite

BackgroundInteractions of glycoconjugates with endogenous galectins, have been long proposed to participate in several reproductive processes including implantation. In human placenta gal-1, gal-3, gal-8, and gal-13 proteins are known to be present. Each of them has been proposed to play multiple functions, but so far no clear picture has emerged. We hypothesized that gal-1 participates in trophoblast invasion, and conducted Matrigel invasion assay using isolated cytotrophoblast from first trimester placenta and HTR-8/SVneo cell line to test it.Methods and FindingsFunction blocking anti-gal-1 antibody was employed to assess participation of endogenous gal-1 in cell adhesion, cell invasion of HTR-8/SVneo cells. When gal-1 was blocked in isolated trophoblast cell invasion was reduced to 75% of control (SEM±6.3, P<0.001) and to 66% of control (SEM±1.7, P<0.001) in HTR-8/SVneo cell line. Increased availability of gal-1, as two molecular forms of recombinant human gal-1 (CS-gal-1 and Ox-gal-1), resulted in increased cell invasion by cytotrophoblast to 151% (SEM±16, P<0.01) with 1 ng/ml of CS-gal-1, and to 192% (SEM±51, P<0.05) with 1 µg/ml of Ox-gal-1. Stimulation was also observed in HTR-8/SVneo cells, to 317% (SEM±58, P<0.001) by CS-gal-1, and to 200% (SEM±24, P<0.001) by Ox-gal-1 at 1 µg/ml. Both sets of results confirmed involvement of gal-1 in trophoblast invasion. Galectin profile of isolated cytotrophoblast and HTR-8/SVneo cells was established using RT-PCR and real-time PCR and found to consist of gal-1, gal-3 and gal-8 for both cell types. Only gal-1 was located at the trophoblast cell membrane, as determined by FACS analysis, which is consistent with the results of the functional tests.Conclusion and SignificanceThese findings qualify gal-1 as a member of human trophoblast cell invasion machinery.

show abstract

Section: Methodsmentioning

confidence: 99%

Galectin-1 Is Part of Human Trophoblast Invasion Machinery - A Functional Study In Vitro

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The main function of HCG is to delay the apoptosis of the corpus luteum during the first trimester of pregnancy. HCG has several paracrine effects in the process of implantation (9), angiogenesis and placentation (10, 11), and development of maternal immunotolerance (12). Low level and non-exponential increase of HCG in maternal serum during the first trimester of the pregnancy is a clinically accepted risk factor for miscarriage (13-15).…”

Section: Introductionmentioning

confidence: 99%