Carboxypeptidase�E in rat antropyloric mucosa: distribution in progenitor and mature endocrine cell types

Hougaard, David M.; Larsson, Lars‐Inge

doi:10.1007/s00418-003-0606-4

Cited by 8 publications

(10 citation statements)

References 49 publications

(47 reference statements)

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“…52 Carboxypeptidase E is a key enzyme for pro-gonadotropin-releasing hormone processing 53 and is also involved in gut hormone processing. 54 Addition of follicle-stimulating hormone to Sertoli cells increased the MMP-14 mRNA level, implicating a role in spermatogenesis. 55 Genes of C3, hormone-sensitive lipase and peroxisome proliferative-activated receptor-gamma may exert a modifying effect on lipid and glucose metabolism in familial combined hyperlipidemia.…”

Section: Resultsmentioning

confidence: 98%

Global analysis of differentially expressed genes in androgen-independent prostate cancer

Tang

et al. 2007

Prostate Cancer Prostatic Dis

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Progression to androgen independent (AI) is the main cause of death in prostate cancer, and the mechanism is still unclear. By reviewing the expression profiles of 26 prostate cancer samples in a holistic view, we found a group of genes differentially expressed in AI compared with androgen-dependent groups (P-value<0.01, t-test). Focusing on apoptosis, proliferation, hormone and angiogenesis, we found a group of genes such as thioredoxin domain containing 5 , tumor necrosis factor receptor superfamily, member 10a , ribosomal protein S19 and Janus kinase 2 upregulated in AI prostate cancer, could play important roles in the transition from AD to AI and could be biomarkers of prognosis.

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Section: Resultsmentioning

confidence: 98%

Global analysis of differentially expressed genes in androgen-independent prostate cancer

Tang

et al. 2007

Prostate Cancer Prostatic Dis

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“…Although CPE has been detected in enteroendocrine cells of the upper GIT [14], nothing is known about CPE expression in the lower intestines. Therefore, we performed a CPE-specific staining of intestinal biopsies showing that CPE is located in defined cells of the ileal and colonic epithelium.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, there is increasing evidence for a CPE expression in EEC [14] implicating a functional role for EEC function and therefore for intestinal homeostasis and barrier function.…”

Section: Introductionmentioning

confidence: 99%

Carboxypeptidase E Modulates Intestinal Immune Homeostasis and Protects against Experimental Colitis in Mice

et al. 2014

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Enteroendocrine cells (EEC) produce neuropeptides, which are crucially involved in the maintenance of the intestinal barrier. Hence, EEC dysfunction is suggested to be involved in the complex pathophysiology of inflammatory bowel disease (IBD), which is characterized by decreased intestinal barrier function. However, the underlying mechanisms for EEC dysfunction are not clear and suitable models for a better understanding are lacking. Here, we demonstrate that Carboxypeptidase E (CPE) is specifically expressed in EEC of the murine colon and ileum and that its deficiency is associated with reduced intestinal levels of Neuropeptide Y (NPY) and Peptide YY (PYY), which are both produced by EEC. Moreover, cpe−/− mice exhibit an aggravated course of DSS-induced chronic colitis compared to wildtype littermates. In addition, we observed elevated mucosal IL-6 and KC transcript levels already at baseline conditions in cpe−/− mice. Moreover, supernatants obtained from isolated intestinal crypts of cpe−/− mice lead to increased IL-6 and KC expression in MODE-K cells in the presence of LPS. This effect was reversible by co-administration of recombinant NPY, suggesting a CPE mediated immunosuppressive effect in the intestines by influencing the processing of specific neuropeptides. In this context, the chemotaxis of bone marrow derived macrophages towards respective supernatants was enhanced. In conclusion, our data point to an anti-inflammatory role of CPE in the intestine by influencing local cytokine levels and thus regulating the migration of myeloid immune cells into the mucosa. These findings highlight the importance of EEC for intestinal homeostasis and propose EEC as potential therapeutic targets in IBD.

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“…Carboxypeptidase E (CPE) is believed to be responsible for the peptide cleavage. Hougaard and Larsson (2004) studied the presence of this enzyme in the antropyloric mucosa of rat. They reported that gastrin cells and progenitor gastrin/ somatostatin cells produce CPE, whereas mature somatostatin and serotonin cells do not.…”

Section: Enteroendocrine Cellsmentioning

confidence: 99%

News and views in Histochemistry and Cell Biology

Asan¹,

Drenckhahn²

2004

Histochem Cell Biol

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Advances in histochemical methodology and ingenious applications of novel and improved methods continue to confirm the standing of morphological means and approaches in research efforts, and contribute significantly to increasing our knowledge about structures and functions in all areas of the life sciences from cell biology to pathology. Reports published during recent months documenting this progress are summarized in the present review.

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Carboxypeptidase�E in rat antropyloric mucosa: distribution in progenitor and mature endocrine cell types

Cited by 8 publications

References 49 publications

Global analysis of differentially expressed genes in androgen-independent prostate cancer

Global analysis of differentially expressed genes in androgen-independent prostate cancer

Carboxypeptidase E Modulates Intestinal Immune Homeostasis and Protects against Experimental Colitis in Mice

News and views in Histochemistry and Cell Biology

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