Carboxymethylpachymaran entrapped plant-based hollow microcapsules for delivery and stabilization of β-galactosidase

Deng, Zhao; Pei, Yaqiong; Wang, Shishuai; Zhou, Bin; Li, Jiwei; Hou, Xinyao; Li, Jing; Li, Bin; Liang, Hongshan

doi:10.1039/c9fo00649d

Cited by 19 publications

(20 citation statements)

References 47 publications

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“…However, for the zein/TA system, the RA of β-Gal stayed at about 2% at 3 h in SGC, and reached its peak at about 70% at 24 h in SGC. 22 Hence, the zein/TA system is superior to the previous polysaccharide systems in sustained release of β-Gal. This was due to the thick coating and strong cohesion of the polymer chains, and the β-Gal would be embedded in the zein/TA layer to avoid immediate release into the SIF.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In vitro release behavior was measured using the method reported previously. 22 Briefly, the samples were blended with simulated gastric fluid (SGF, 0.01 M HCl solution, 3.20 mg/mL pepsin) for 2 h and then mixed with simulated intestinal fluid (SIF, pH 7.4 phosphate buffer, 1.60 mg/mL pancreatin). One mL of simulated solution was collected at different time intervals and the enzyme activity was determined.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Some polymer coatings such as enteric coating, calcium alginate coating, and carboxymethyl (1 → 3)-β- d -glucan (CMP) coating have been utilized to address this limitation. These coatings can protect the encapsulated macromolecules from the negative effect of the harsh stomach environment and then release them in the small intestine. , Despite certain progress in the development of coating approaches, it remains a challenge to develop a cost-effective coating system that can achieve sustained small intestinal release of bioactive macromolecules to date.…”

Section: Introductionmentioning

confidence: 99%

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Tuning of Molecular Interactions between Zein and Tannic Acid to Modify Sunflower Sporopollenin Exine Capsules: Enhanced Stability and Targeted Delivery of Bioactive Macromolecules

Deng

Wang

Pei

et al. 2021

ACS Appl. Bio Mater.

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There are multiple obstacles for the storage and digestion of orally administered bioactive macromolecules. This study developed a low-cost and sustained-release delivery system (sporopollenin exine capsules with zein/tannic acid modification) of proteins with excellent storage stability, and at the same time provided insights into the sustained-release mechanism through exploring the interaction between zein and tannic acid (TA). β-Galactosidase (β-Gal) was utilized as a model protein and loaded into sporopollenin exine capsules (SECs), which were then coated with the zein/TA system. Under the optimized zein/TA conditions, the zein/TA system showed better performance than the zein alone system in the sustained release of β-Gal, with the residual activity of about 70.26% after 24 h of simulated digestion. Evaluation of the storage stability demonstrated a β-Gal residual activity of nearly 90% for 28 days at 25 °C. Additionally, FTIR analysis demonstrated that the stability of the zein/TA system depends on both hydrogen bonding and certain covalent bonding through the Schiff-base reaction, and the sustained release is regulated by the bonding strength.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tuning of Molecular Interactions between Zein and Tannic Acid to Modify Sunflower Sporopollenin Exine Capsules: Enhanced Stability and Targeted Delivery of Bioactive Macromolecules

Deng

Wang

Pei

et al. 2021

ACS Appl. Bio Mater.

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“…This type of microgel has also been utilized to encapsulate and protect pancreatic lipase from degradation under gastric conditions [78]. Various other kinds of CDSs have also been developed to protect lactase under gastrointestinal conditions, including biopolymer microgels [125,150,151], lipid microparticles [152], and plant-based microcapsules [153].…”

Section: Digestive Enzymesmentioning

confidence: 99%

Recent Advances in Encapsulation, Protection, and Oral Delivery of Bioactive Proteins and Peptides using Colloidal Systems

Perry

McClements

2020

Molecules

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There are many areas in medicine and industry where it would be advantageous to orally deliver bioactive proteins and peptides (BPPs), including ACE inhibitors, antimicrobials, antioxidants, hormones, enzymes, and vaccines. A major challenge in this area is that many BPPs degrade during storage of the product or during passage through the human gut, thereby losing their activity. Moreover, many BPPs have undesirable taste profiles (such as bitterness or astringency), which makes them unpleasant to consume. These challenges can often be overcome by encapsulating them within colloidal particles that protect them from any adverse conditions in their environment, but then release them at the desired site-of-action, which may be inside the gut or body. This article begins with a discussion of BPP characteristics and the hurdles involved in their delivery. It then highlights the characteristics of colloidal particles that can be manipulated to create effective BPP-delivery systems, including particle composition, size, and interfacial properties. The factors impacting the functional performance of colloidal delivery systems are then highlighted, including their loading capacity, encapsulation efficiency, protective properties, retention/release properties, and stability. Different kinds of colloidal delivery systems suitable for encapsulation of BPPs are then reviewed, such as microemulsions, emulsions, solid lipid particles, liposomes, and microgels. Finally, some examples of the use of colloidal delivery systems for delivery of specific BPPs are given, including hormones, enzymes, vaccines, antimicrobials, and ACE inhibitors. An emphasis is on the development of food-grade colloidal delivery systems, which could be used in functional or medical food applications. The knowledge presented should facilitate the design of more effective vehicles for the oral delivery of bioactive proteins and peptides.

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“…Treating dairy products with β-galactosidase through pre-hydrolyzation that can reduce lactose concentration offers a promising solution ( Li et al, 2020 ). Thus far, numerous β-galactosidases were purified, cloned, and characterized from bacteria ( Mavromatis et al, 2010 ; Tian et al, 2013 ), fungi ( Rico-Díaz et al, 2017 ), yeast ( de Freitas et al, 2020 ), plants ( Deng et al, 2019 ), and mammals ( He et al, 2008 ). Among all sources, microbial β-galactosidases have drawn extensive attraction due to their high yields, high activity, and abundance ( Oliveira et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization and Application of a New β-Galactosidase Gal42 From Marine Bacterium Bacillus sp. BY02

Zhou

Han

et al. 2021

Front. Microbiol.

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β-Galactosidase plays an important role in medicine and dairy industry. In this study, a new glycoside hydrolase family 42 (GH42) β-galactosidase-encoding gene, gal42, was cloned from a newly isolated marine bacterium Bacillus sp. BY02 and expressed in Escherichia coli. Structural characterization indicated that the encoding β-galactosidase, Gal42, is a homotrimer in solution, and homology modeling indicated that it retains the zinc binding sites of the Cys cluster. The reaction activity of Gal42 was significantly increased by Zn2+ (229.6%) and other divalent metal ions (Mn2+, Mg2+, and Co2+), while its activity was inhibited by EDTA (53.9%). Meanwhile, the thermo-stability of the Gal42 was also significantly enhanced by 5 and 10 mM of zinc ion supplement, which suggested that the “Cys-Zn” motif played important roles in both structural stability and catalytic function. Furthermore, Gal42 showed effective lactose hydrolysis activity, which makes the enzyme hydrolyze the lactose in milk effectively. These properties make Gal42 a potential candidate in food technology.

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Carboxymethylpachymaran entrapped plant-based hollow microcapsules for delivery and stabilization of β-galactosidase

Abstract: β-Galactosidase (β-Gal) as a dietary supplement can alleviate symptoms of lactose intolerance.

Cited by 19 publications

References 47 publications

Tuning of Molecular Interactions between Zein and Tannic Acid to Modify Sunflower Sporopollenin Exine Capsules: Enhanced Stability and Targeted Delivery of Bioactive Macromolecules

Tuning of Molecular Interactions between Zein and Tannic Acid to Modify Sunflower Sporopollenin Exine Capsules: Enhanced Stability and Targeted Delivery of Bioactive Macromolecules

Recent Advances in Encapsulation, Protection, and Oral Delivery of Bioactive Proteins and Peptides using Colloidal Systems

Characterization and Application of a New β-Galactosidase Gal42 From Marine Bacterium Bacillus sp. BY02

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