Carboxymethylcellulose: a binder for thin-layer chromatography of lipids and indoles

Obreiter, Jean B.; Stowe, Bruce B.

doi:10.1016/s0021-9673(01)82464-x

Cited by 24 publications

(5 citation statements)

References 9 publications

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“…The occurrence of IAA in infinitesimal concentrations in most hi'gher plant tissuies, the relaitively small yields obtained from in vitro biosynthesis, and the lability of the compotund in liight, 02 atmosphere and strong acid soluitionis pose = , problems that have never been solved entirely 8 9 10 satisfactorily. Problems in identification and analysis of IAA continue to exist despite the development of many valuable proce(lures involving paper B (2,5,7,12,13,15,23,33,34), thin-layer (17,21,22) and partition column (15,24,25,26) chromatography; electrophoresis (15, 23); gas chromatography (26); s9pectrophotofltiorometry (26); colorimetry (2,5,7,12,13,15,23,24,26,33,34) and spectrophotometry (7).…”

Section: Discussionmentioning

confidence: 99%

Biosynthesis of Indoleacetic Acid from Tryptophan-¹⁴C in Cell-free Extracts of Pea Shoot Tips

Moore

Shaner

1967

Plant Physiol.

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Summary. A 2-step, 1-d:,mens-ional thn-layer chromatograph-c proceduire fnr isolating indoleacetic ac:d (IAA) was developed and util:zed in inve,t at:ons of the biosynthesis of JAA from tryl)to,)han-_4C in cell-fre'-extracts of ipea (Pi.sun sativitmn L.) shoot tips. Identfcat;on of a 14C-prodnict as IAA was bv (a) co-chromatograT)hv of atitheiiitc IAA and '4C-pro(duict on th.:n-layer chromatography, and (b) gas-liqu-;d and thin-laver chromategraphy of authentic and presumptive IAA methyl esters. Dalysis of enzyme extracts and addt;on of a-keto-li1tqr`c ac d anl pvr:doxal phosphate to reactioni m;xtl'res re,ulited in ap)prox:mately 2-to 3-f-ld increases in net y;elds of IAA over yields in non-d:alyzed react-on m:xtres wW:ch d:d not conta;n ad,d:tives esqent:al to a translaminace reaction of trvptophan. Add`t:on of thiam:ne pyro0hoonbh,,te to reacton m;xtl,req fiurther enhanced npt b;h,vnth-s-s of I'A. It is conclhlded that the format;on of indolepyrtiv:c ac;d and its silhbenlent decarboxvlat:on prohablv are seeventtal react:onis in the maior pathway of IAA bocvnfhsis from trvntonhan in cell-free extracts of Pisutnt short t:pS. ComDar;-on of max:mtlm n't-IAA biosynthes;s in extracts of shoot t:ps of etiolated anid leht-grown dwvarf and tall pea !dluui;nzs reveal-id. aln order, on a uin:t T)rofe:n N ba:, of: 1`lo-4-rown tall > Pght-zrown dwarf > etiolated tall _ etolated dwarf. It is conclrxded that the d:fferent rates of stpm elonzation among et;el"ted and l:ght-pr-wrn dwarf and tal!l pea seedl:ngs are correlated, in general, with differences in net TAA biosynthesis aild setisiitivity of the tissules to IAA.Ex'olan.utions for the (Ifferer(nces in stemi elomioatioIl betweeil dwarf and(l tall varne;es of a snecies e.g. Pisumin sativ'mi, and betweon et;olatod atnd tlc-ht-grown l)lants of the sam-var;ety have he-n sought repeatedly throu"gh inve-t;zat:ons of pols):bVe (orrelat;ve difference,s in endogen-onls aulx;n an1d ib,berell;n relationships. The maiori'ty of these investigations have been either: (a) studies of comparat:ve resp)onses of intact see(linzs or excised stem sectioins to exogenotus hormones: or (b) extraction and bWoassay of enidozeniouis hormones. Another kin(d of exoer:mmntal avnroach which is tusefult in attempts to elu1cidate comparative g,rowth hormone relationships is invest:gation of hormone bWosvntheses tsi;ng cell-free enzymne prepatrations. Tn 1958, Gor(lon (12) f:rqt 'describe-d an enzyme system from shoot ti'ps of Phascoluts autreus seedlings w,hich catalvzed the coniversion of trvntophan to indole-3-acetic ac&d (IAA). Wghtman ;ind Cohen (39) recently described in detail the intermediary steps in the enzymic conlversion of trvptophan to TA.A in cell-free preparation;s from 1 This investigation was supported in part by Grauit GBI-4613 from the National Science Founidation. mntg be-an (Ph1 rscolls (1a1 remis) seedlPl;ngs. So)me propert e-of an TAA-syntheq;z;ng syvtem isol-te-d from ani:ces of Avona. coleoptiles and otllel-W'ant mrn-er:akc were de-cr'be,l by Lant.can and Mui:r (17). WhIle cel...

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Section: Discussionmentioning

confidence: 99%

Biosynthesis of Indoleacetic Acid from Tryptophan-¹⁴C in Cell-free Extracts of Pea Shoot Tips

Moore

Shaner

1967

Plant Physiol.

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“…The plates were activated by heating at 110°C for 20 min and allowed to cool to room temperature prior to use. A mixture of chloroform and acetic acid (95 :5, v/v) was used for separating the acid auxins while for the neutral indoles, n-hexane and 2-butanone in the proportion of 7:3 was used as recommended by Obreiter and Stowe (1964). p-Dimethylaminocinnamaldehyde (DMCA) -the spraying agent used for detecting indoleswas prepared as follows: 2 g of DMCA were dissolved in a mixture of 100 ml 6 N HCl and 100 ml of 96 percent ethanol.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of Indole‐3‐acetaldehyde.

Rajagopal

1967

Physiologia Plantarum

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Aerobic infiltration of synthetic indoleacetaldehyde (IAAld) in buffered medium of pH 4.55, into living tissues of lower and higher plants, leads in the majority of cases to the formation of both indoleacetic acid and tryptophol. This activity is evinced by etiolated as well as green tissues. Besides, all parts of higher plants tested — roots, cotyledons, hypocotyls and leaves possess this activity. Abolishment of this activity by boiling indicates its enzymic nature. Coupled with the established occurrence of indoleacetaldehyde, the widespread distribution of such activity, strengthens the probability that indoleacetaldehyde may be the normal and natural precursor in the biosynthesis of indoleacetic acid.

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“…The report oftryptamine production by the fungal plant pathogens Taphrina deforman8 and Dibotryon morbo8um (Crady & Wolf, 1959) must be discounted, for the compound 'identified' was, by the design of the extraction procedure, either an acidic or a neutral indole. A recent examination of Taphrina metabolism confirms this conclusion (Perley & Stowe, 1966).…”

mentioning

confidence: 77%

“…Thin-layer chromatography. The methods of Obreiter & Stowe (1964) were employed to achieve the thin-layer resolution of the extracted compounds. In each case where identifications have been made, chromatographic agreement with authentic compounds was obtained in a minimum of three solvents.…”

Section: Usamentioning

confidence: 99%

The production of tryptamine from tryptophan by Bacillus cereus (KVT)

Perley¹,

Stowe²

1966

Biochemical Journal

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1. A strain of Bacillus cereus has been isolated that can produce tryptamine when grown in a broth containing tryptophan. 2. The conditions of culture under which this conversion is optimum, as well as the general pathways of tryptophan metabolism by this micro-organism, have been examined, and the information obtained has been used to obtain the first demonstration of cell-free tryptophan-carboxy-lyase activity. 3. The significance of these findings both to the current attempts to elucidate the pathways of metabolism of tryptophan in higher plants and to the published generalizations about the previously studied amino acid carboxy-lyases is discussed.

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Carboxymethylcellulose: a binder for thin-layer chromatography of lipids and indoles

Cited by 24 publications

References 9 publications

Biosynthesis of Indoleacetic Acid from Tryptophan-¹⁴C in Cell-free Extracts of Pea Shoot Tips

Biosynthesis of Indoleacetic Acid from Tryptophan-¹⁴C in Cell-free Extracts of Pea Shoot Tips

Metabolism of Indole‐3‐acetaldehyde.

The production of tryptamine from tryptophan by Bacillus cereus (KVT)

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Carboxymethylcellulose: a binder for thin-layer chromatography of lipids and indoles

Cited by 24 publications

References 9 publications

Biosynthesis of Indoleacetic Acid from Tryptophan-14C in Cell-free Extracts of Pea Shoot Tips

Biosynthesis of Indoleacetic Acid from Tryptophan-14C in Cell-free Extracts of Pea Shoot Tips

Metabolism of Indole‐3‐acetaldehyde.

The production of tryptamine from tryptophan by Bacillus cereus (KVT)

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Biosynthesis of Indoleacetic Acid from Tryptophan-¹⁴C in Cell-free Extracts of Pea Shoot Tips

Biosynthesis of Indoleacetic Acid from Tryptophan-¹⁴C in Cell-free Extracts of Pea Shoot Tips