Carboxymethyl chitosan incorporated with gliadin/phlorotannin nanoparticles enables the formation of new active packaging films

Zhao, Jianbo; Jiang, Haixin; Huang, Qiuli; Xu, Jingting; Duan, Mengxia; Yu, Sophia; Zhi, Zijian; Pang, Jie; Wu, Chunhua

doi:10.1016/j.ijbiomac.2022.01.128

Cited by 29 publications

(5 citation statements)

References 38 publications

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“…Compared to results for other groups, the TG loss of the CON was lowest and the largest TG loss was at 0P after 150 °C, indicating the thermal stability of CEP was reduced during thermal treatment. It could be because of the denaturation of enzyme protein during thermal treatment . With the increasing concentration of PhTE, the weight loss of CEP gradually increased and was lower than 0P, which might be attributable to the rapid dissolution of excessive PhTE covering the surface of the proteases aggregate after aggregation, leading to the increase of TG loss .…”

Section: Results and Discussionmentioning

confidence: 99%

“…It could be because of the denaturation of enzyme protein during thermal treatment. 46 With the increasing concentration of PhTE, the weight loss of CEP gradually increased and was lower than 0P, which might be attributable to the rapid dissolution of excessive PhTE covering the surface of the proteases aggregate after aggregation, leading to the increase of TG loss. 47 These results suggested that the addition of PhTE could promote the CEP from weight losses and the thermal treatment could further reduce the thermal stability of CEP.…”

Section: Sds-pagementioning

confidence: 95%

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Phlorotannin Extracts from Ascophyllum nodosum Inhibited Proteases Activities and Structural Changes from Apostichopus japonicus

Wang

Xie

et al. 2022

ACS Food Sci. Technol.

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Fresh Apostichopus japonicas (A. japonicas) are easy to autolyze through the actions of endogenous proteases under certain circumstances, further inducing a decline in A. japonicas product quality and business value. To solve this serious problem in the A. japonicas industry, we investigated the inhibition of phlorotannin extracts (PhTE) on the activities and structural changes of crude endogenous proteinase (CEP) from A. japonicas. The results of enzyme activity and SDS-PAGE indicated that PhTE could inhibit enzyme activity in a dose dependent manner. Analysis of multiple spectroscopic methods, scanning electron microscopy, and differential scanning calorimetry indicated that PhTE promoted the cross-linking and aggregation of CEP and increased the particle size of the enzyme, thus changing the structure of the enzyme protein and decreasing the thermostability of protease. In conclusion, PhTE might be an effective inhibitor on endogenous proteinase in A. japonicas, which opened up a new way to prevent the A. japonicas autolysis.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Sds-pagementioning

confidence: 95%

Phlorotannin Extracts from Ascophyllum nodosum Inhibited Proteases Activities and Structural Changes from Apostichopus japonicus

Wang

Xie

et al. 2022

ACS Food Sci. Technol.

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“…An Attenuated total reflection-Fourier transformed infrared (ATR-FTIR) spectrophotometer (Thermo Fisher Scientific Co., Ltd., Stoughton, MA, USA) was used to study the chemical structure of the films. The as-obtained membranes were directly scanned in the range of 4000-400 cm −1 at a resolution of 4 cm −1 [40]. X-ray photoelectron spectroscopy (XPS) measurements were carried out employing a Theta Probe angle-resolved XPS system, which utilized monochromatized Al Kα X-ray radiation.…”

Section: Characterizationmentioning

confidence: 99%

In Situ Preparation of Chlorine-Regenerable Antimicrobial Polymer Molecular Sieve Membranes

Zhang,

Qian,

Wen

et al. 2024

Molecules

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Microbial contamination has profoundly impacted human health, and the effective eradication of widespread microbial issues is essential for addressing serious hygiene concerns. Taking polystyrene (PS) membrane as an example, we herein developed report a robust strategy for the in situ preparation of chlorine-regenerable antimicrobial polymer molecular sieve membranes through combining post-crosslinking and nucleophilic substitution reaction. The cross-linking PS membranes underwent a reaction with 5,5-dimethylhydantoin (DMH), leading to the formation of polymeric N-halamine precursors (PS-DMH). These hydantoinyl groups within PS-DMH were then efficiently converted into biocidal N-halamine structures (PS-DMH-Cl) via a simple chlorination process. ATR-FTIR and XPS spectra were recorded to confirm the chemical composition of the as-prepared PS-DMH-Cl membranes. SEM analyses revealed that the chlorinated PS-DMH-Cl membranes displayed a rough surface with a multitude of humps. The effect of chlorination temperature and time on the oxidative chlorine content in the PS-DMH-Cl membranes was systematically studied. The antimicrobial assays demonstrated that the PS-DMH-Cl membranes could achieve a 6-log inactivation of E. coli and S. aureus within just 4 min of contact time. Additionally, the resulting PS-DMH-Cl membranes exhibited excellent stability and regenerability of the oxidative chlorine content.

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“…The transmittance of HML@CMCS/PVA was significantly higher than that of HML@CMCS after 400 nm due to the difference in film thickness and polymer crystallinity, confirming that the incorporation of PVA improved the transmittance of CMCS, and this is numerically much lower than commercial films. Meanwhile, within the range of 200-400 nm, the shielding ability of HML@CMCS/PVA against UV light was significantly elevated due to CMCS, which helped mitigate its effect on the stability of pesticides in the films [50]. The opacities of HML@CMCS, HML@/PVA, and HML@CMCS/PVA were compared to those of commercial film at 600 nm in Figure 5B.…”

Section: Optical Properties Of Filmsmentioning

confidence: 99%

Biodegradable Carboxymethyl Chitosan/Polyvinyl Alcohol Hymexazol-Loaded Mulch Film for Soybean Root Rot Control

Lv,

Meng,

Sun

et al. 2023

Agronomy

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Soybean root rot is an important disease that has affected soybean crops in recent years, but the available control methods still do not provide good protection for soybeans. In this study, three biodegradable mulch films (HML@CMCS, HML@PVA, HML@CMCS/PVA) were prepared from carboxymethyl chitosan (CMCS) and polyvinyl alcohol (PVA), and hymexazol (HML) was loaded on them. The surface and cryofractured surface were compared via micromorphological observations using SEM, AFM, and 3D measurements. Mechanical, water barrier, and optical properties were compared between the different films, and the results showed that the composite film (HML@CMCS/PVA) had better tensile properties, lower water content (35%), and lower water solubility (28%), while water barrier properties were significantly improved. HML@CMCS/PVA also had better light transmission than commercial films (opacity at 600 nm of 1.92). Finally, the composite film was selected to study antifungal properties, soil degradability, and biosafety. The results showed that it exhibited significant inhibitory activity against Fusarium oxysporum and could effectively control soybean root rot, while degradation reached 58% after 7 d of soil burial, and the mortality of earthworms relative to the microplastics produced by the film within 7 d (23.33%) was much lower than that of the commercial film, demonstrating its excellent biosafety. This study provides a new approach for the on-farm management of soybean root rot and the reduction in soil microplastic pollution.

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Carboxymethyl chitosan incorporated with gliadin/phlorotannin nanoparticles enables the formation of new active packaging films

Cited by 29 publications

References 38 publications

Phlorotannin Extracts from Ascophyllum nodosum Inhibited Proteases Activities and Structural Changes from Apostichopus japonicus

Phlorotannin Extracts from Ascophyllum nodosum Inhibited Proteases Activities and Structural Changes from Apostichopus japonicus

In Situ Preparation of Chlorine-Regenerable Antimicrobial Polymer Molecular Sieve Membranes

Biodegradable Carboxymethyl Chitosan/Polyvinyl Alcohol Hymexazol-Loaded Mulch Film for Soybean Root Rot Control

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