Carboxymethyl cellulose and glycerol act synergistically as cryoprotectant during cryopreservation of ram semen

Paul, Rajani Kr.; Kumar, Davendra; Singh, Randhir

doi:10.1016/j.cryobiol.2021.06.001

Cited by 5 publications

(9 citation statements)

References 27 publications

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“…In conclusion, the severe malformation, excessive ROS generation, and poor cell viability of DMSO-cultured cells could be probably due to the widely acknowledged cytotoxicity of DMSO, which might further explain why DMSO is rarely used for cryopreserving food-related microorganisms. Besides, although glycerol was reported to show cytotoxicity at high concentration, and about 5% cell malformation was indeed observed in this study, the glycerol-cultured cells showed no difference ( p > 0.01) regarding ROS accumulation and cell viability as compared with the fresh ones.…”

Section: Resultsmentioning

confidence: 45%

“…Currently, glycerol is still the mainstream cryoprotectant for cryopreserving food-related microorganisms, while glycerol is a relatively weak cryoprotectant and shows relatively poor penetrability even if it is classified as a typical penetrating cryoprotectant, , affecting its cryoprotective effects. Besides, glycerol also exerts cytotoxicity at higher concentrations, causing potential food safety issues. Hence, ecofriendly, non-toxic, and biodegradable cryoprotectants that can meet the strict “ingestion safety” in the food industry is increasingly attracting concerns.…”

Section: Introductionmentioning

confidence: 99%

“…For the food-related microorganisms, Qiao et al reported five NADESs as alternative cryoprotectants to preserve lactic acid bacteria for a long-term cryostorage, and the NADES prepared from glycerol and l -proline was observed to better maintain the cell membrane structures and retain activities of intracellular enzymes, declaring the potential of NADESs as green cryoprotectants . However, most of these works more or less involve glycerol, which can still exert cytotoxicity at higher concentrations and may cause potential food safety issues . It is to be noted that Craverio et al have already presented a pioneering work for mammalian cell cryopreservation using proline-/glucose-based NADESs, proving the low toxicity and potential of glycerol-free NADESs as emerging cryoprotectants.…”

Section: Introductionmentioning

confidence: 99%

“…30 However, most of these works more or less involve glycerol, which can still exert cytotoxicity at higher concentrations and may cause potential food safety issues. 17 It is to be noted that Craverio et al have already presented a pioneering work for mammalian cell cryopreservation using proline-/glucose-based NADESs, 25 proving the low toxicity and potential of glycerol-free NADESs as emerging cryoprotectants. This implies that NADESs consisting of common metabolites that are abundant in biosystems may attract more interest for cryopreserving food-related microorganisms.…”

Section: ■ Introductionmentioning

confidence: 99%

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Bioinspired Cryoprotectants Enabled by Binary Natural Deep Eutectic Solvents for Sustainable and Green Cryopreservation

Tian

Sun

et al. 2022

ACS Sustainable Chem. Eng.

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Cryopreservation plays an important role in prolonging the cell viability, but non-negligible cytotoxicity of cryoprotectants restrains its further development in many areas with safety concerns such as the food industry. Inspired by cold-tolerant animals, three binary natural deep eutectic solvents (NADESs) were prepared and used as cryoprotectants for achieving cytotoxicity-free and green cryopreservation, whose cryoprotective efficiency was compared with those of glycerol and dimethyl sulfoxide (DMSO), using yeast Saccharomyces cerevisiae, a common commercial microorganism in the food industry, as the cryopreservation object. Cytotoxicity evaluation involving reactive oxygen species accumulation and cell viability showed that the NADESs could be safe and biocompatible. In addition, cytomorphology indicated that the cells cryoprotected by NADESs show plump profiles with smooth cytoderms, while rough cytoderms or even severe cell distortion was partially observed for the ones cryopreserved by glycerol and DMSO. The live/apoptosis/death test of resuscitated cells based on flow cytometry illustrated that the NADES-cryopreserved cells could show higher cell viability (75−80%) with almost no apoptosis (<1.0%). Mechanisms of NADES-supported cryopreservation were further investigated concerning antifreezing capacities and ice recrystallization inhibition activities of NADESs, which suggested that NADESs could exhibit reinforced hydrogen-bonding strengths when decreasing temperature and correspondingly restrain the water molecular motion, making NADESs effectively inhibit ice recrystallization and prevent cells from ice-induced mechanical damage. The excellent cryoprotective efficiency of NADESs may help to open up new avenues for green cryopreservation and attract more concerns for NADESs as emerging cryoprotectants.

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Section: Resultsmentioning

confidence: 45%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bioinspired Cryoprotectants Enabled by Binary Natural Deep Eutectic Solvents for Sustainable and Green Cryopreservation

Tian

Sun

et al. 2022

ACS Sustainable Chem. Eng.

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show abstract

“…9 In addition, they can get inside cells to adjust osmotic pressure and decrease osmotic damage. 10 Permeable glycerol, 11 dimethyl sulfoxide (DMSO), 12 and ethylene glycol (EG) 13 fall under this category. The latter act in extracellular to work by promoting cell dehydration to inhibit the growth of intracellular ice and also protect cells from extracellular ice crystals' damage, 9 including trehalose, 14 hydroxyethyl starch (HES), 15 polyampholytes, 16 poly(vinyl)alcohol (PVA), 17 and so forth.…”

Section: Introductionmentioning

confidence: 99%

Trehalose in Biomedical Cryopreservation–Properties, Mechanisms, Delivery Methods, Applications, Benefits, and Problems

Liu

et al. 2023

ACS Biomater. Sci. Eng.

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Cells and tissues are the foundation of translational medicine. At present, one of the main technological obstacles is their preservation for long-term usage while maintaining adequate viability and function. Optimized storage techniques must be developed to make them safer to use in the clinic. Cryopreservation is the most common long-term preservation method to maintain the vitality and function of cells and tissues. But, the formation of ice crystals in cells and tissues is considered to be the main mechanism that could harm cells and tissues during freezing and thawing. To reduce the formation of ice crystals, cryoprotective agents (CPAs) must be added to the cells and tissues to achieve the cryoprotective effect. However, conventional cryopreservation of cells and tissues often needs to use toxic organic solvents as CPAs. As a result, cryopreserved cells and tissues may need to go through a time-consuming washing process to remove CPAs for further applications in translational medicine, and multiple valuable cells are potentially lost or killed. Currently, trehalose has been researched as a nontoxic CPA due to its cryoprotective ability and stability during cryopreservation. Nevertheless, trehalose is a nonpermeable CPA, and the lack of an effective intracellular trehalose delivery method has become the main obstacle to its use in cryopreservation. This article illustrated the properties, mechanisms, delivery methods, and applications of trehalose, summarized the benefits and limits of trehalose, and summed up the findings and research direction of trehalose in biomedical cryopreservation.

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Study on the barrier properties and antibacterial properties of cellulose-based multilayer coated paperboard used for fast food packaging

Wang

Zhang

et al. 2022

Food Bioscience

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Carboxymethyl cellulose and glycerol act synergistically as cryoprotectant during cryopreservation of ram semen

Cited by 5 publications

References 27 publications

Bioinspired Cryoprotectants Enabled by Binary Natural Deep Eutectic Solvents for Sustainable and Green Cryopreservation

Bioinspired Cryoprotectants Enabled by Binary Natural Deep Eutectic Solvents for Sustainable and Green Cryopreservation

Trehalose in Biomedical Cryopreservation–Properties, Mechanisms, Delivery Methods, Applications, Benefits, and Problems

Study on the barrier properties and antibacterial properties of cellulose-based multilayer coated paperboard used for fast food packaging

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