Carboxylicivirga gen. nov. in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. and Carboxylicivirga taeanensis sp. nov., and reclassification of Cytophaga fermentans as Saccharicrinis fermentans gen. nov., comb. nov.

Abstract

Two facultatively anaerobic mesophilic bacteria, strains MEBiC 07026T and MEBiC 08903T, were isolated from two different tidal flat sediments and both strains showed approximately 92.2 % 16S rRNA gene sequence similarity with [Cytophaga] fermentans DSM 9555T. 16S rRNA gene sequence similarity between the two new isolates was 97.5 % but levels of DNA–DNA relatedness between the two were 31.3–31.8 %. Phylogenetic analysis revealed that the two isolates and [Cytophaga] fermentans DSM 9555T were affiliated with the family Marinilabiliaceae in the class Bacteroidia . The dominant fatty acids of strains MEBiC 07026T, MEBiC 08903T and [Cytophaga] fermentans DSM 9555T were branched-type or hydroxylated C15 : 0, but [Cytophaga] fermentans DSM 9555T contained a higher proportion of anteiso-branched fatty acids. The two new isolates contained a markedly higher proportion of monounsaturated fatty acids than other members of the family Marinilabiliaceae . The major respiratory quinone of the strains was MK-7. Strains MEBiC07026T and MEBiC08903T utilized a wide range of carboxylic acids whereas [Cytophaga] fermentans DSM 9555T utilized carbohydrates rather than carboxylic acids. The DNA G+C content of the novel strains was about 44 mol% but that of [Cytophaga] fermentans DSM 9555T revealed from the genome sequence was 37.6 mol%. Based on evidence from this polyphasic taxonomic study, a novel genus, Carboxylicivirga gen. nov., is proposed in the family Marinilabiliaceae with two novel species, Carboxylicivirga mesophila sp. nov. with type strain MEBiC 07026T ( = KCCM 42978T = JCM 18290T) and Carboxylicivirga taeanensis sp. nov. with type strain MEBiC 08903T ( = KCCM 43024T = JCM 19490T). Additionally, [Cytophaga] fermentans DSM 9555T ( = ATCC 19072T) is reclassified as Saccharicrinis fermentans gen. nov., comb. nov.

Carboxylicivirga flava sp. nov., isolated from marine surface sediment

“…T was 44.7 mol% and was consistent with those of members of the genus Carboxylicivirga, which ranged from 40 to 44.2 mol% (Yang et al, 2014;Wang et al, 2015).…”

“…The family Marinilabiliaceae (Ludwig et al, 2011) includes, the time of writing, 10 genera: Alkaliflexus (Zhilina et al, 2004), Alkalitalea (Zhao & Chen, 2012), Anaerophaga (Denger et al, 2002), Carboxylicivirga (Yang et al, 2014), Geofilum (Miyazaki et al, 2012), Mangroviflexus , Marinilabilia (Nakagawa & Yamasato, 1996), Natronoflexus (Sorokin et al, 2011), Saccharicrinis (Yang et al, 2014) and Thermophagus (Gao et al, 2013); and one misclassified species: [Cytophaga] xylanolytica (Haack & Breznak, 1993). The genus Carboxylicivirga was first proposed by Yang et al (2014) comprising two species, Carboxylicivirga mesophila and Carboxylicivirga taeanensis.…”

“…The genus Carboxylicivirga was first proposed by Yang et al (2014) comprising two species, Carboxylicivirga mesophila and Carboxylicivirga taeanensis. A recent study identified a third species in the genus Carboxylicivirga, namely Carboxylicivirga linearis (Wang et al, 2015).…”

Labilibacter aurantiacus gen. nov., sp. nov., isolated from sea squirt (Styela clava) and reclassification of Saccharicrinis marinus as Labilibacter marinus comb. nov.

“…Aminophospholipid was also detected (Fig. S2), which was similar to [Saccharicrinis] marinus Y11 T [25] but unlike S. fermentans DSM 9555 T and S. carchari SS12 T [11,12]. More differential characteristics are shown in Table 2.…”

“…Strains: 1, HQYD1 T (data from this study); 2, [Saccharicrinis] marinus Y11 T (this study); 3, S. fermentans DSM 9555 T (this study); 4, S. carchari SS12 T (this study); 5, Carboxylicivirga mesophila MEBiC 07026 T [11]. All strains were Gram-stain-negative, facultatively anaerobic, rod-shaped.…”