Carboxylic Acids in Wheat, Rye and Barley

Lohaus, Edith; Bios, Inge; Rüdiger, Wolfhart

doi:10.1515/znc-1983-7-805

Cited by 8 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…De novo synthesis of 3HB from acetyl-CoA can be ruled out as 3HB would in this case be labeled at two carbon atoms (M2) instead of four (M4). It has been shown before that 3HB is present in wheat flour in small quantities ( 30 ), further strengthening our hypothesis that 3-hydroxybutyrate was present in the supplemented 13 C-labeled wheat flour and appeared in the circulation after consumption of the WP, but not in GLC. For 2HB, Lohaus et al ( 30 ) reported that it is not present in wheat flour, however, the kinetics closely mirror those of 3HB.…”

Section: Resultssupporting

confidence: 87%

“…It has been shown before that 3HB is present in wheat flour in small quantities ( 30 ), further strengthening our hypothesis that 3-hydroxybutyrate was present in the supplemented 13 C-labeled wheat flour and appeared in the circulation after consumption of the WP, but not in GLC. For 2HB, Lohaus et al ( 30 ) reported that it is not present in wheat flour, however, the kinetics closely mirror those of 3HB. As a second option, 2HB M4 could also be synthesized from threonine via threonine deaminase and 2-ketobutyrate, but these open questions remain to be addressed in future studies.…”

Section: Resultssupporting

confidence: 87%

See 1 more Smart Citation

Systemic Lactate Acts as a Metabolic Buffer in Humans and Prevents Nutrient Overflow in the Postprandial Phase

et al. 2022

View full text Add to dashboard Cite

On an organismal level, metabolism needs to react in a well-orchestrated manner to metabolic challenges such as nutrient uptake. Key metabolic hubs in human blood are pyruvate and lactate, both of which are constantly interconverted by very fast exchange fluxes. The quantitative contribution of different food sources to these metabolite pools remains unclear. Here, we applied in vivo stable isotope labeling to determine postprandial metabolic fluxes in response to two carbohydrate sources of different complexity. Depending on the ingested carbohydrate source, glucose or wheat flour, the net direction of the lactate dehydrogenase, and the alanine amino transferase fluxes were adjusted in a way to ensure sufficient availability, while, at the same time, preventing an overflow in the respective metabolite pools. The systemic lactate pool acts as a metabolic buffer which is fueled in the early- and depleted in the late-postprandial phase and thus plays a key role for systemic metabolic homeostasis.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 87%

Systemic Lactate Acts as a Metabolic Buffer in Humans and Prevents Nutrient Overflow in the Postprandial Phase

et al. 2022

View full text Add to dashboard Cite

show abstract

“…HMG was found conjugated to shikimic acid in the latex of Euphorbia biglandulosa (Euphorbiaceae) (34). In 1983, a quantitative gc analysis of the carboxylic acids found in wheat, rye, and barley seeds indicated the presence of HMG (35).…”

mentioning

confidence: 99%

Identification of 3-Hydroxy-3-methylglutaric Acid (HMG) as a Hypoglycemic Principle of Spanish Moss (Tillandsia usneoides)

Witherup¹,

McLaughlin²,

Judd³

et al. 1995

J. Nat. Prod.

View full text Add to dashboard Cite

Bioactivity-directed fractionation, using brine shrimp lethality and murine hypoglycemia, of an ethanol extract prepared from Tillandsia usneoides, led to the isolation of four apparently bioactive compounds from the water-soluble fraction. The compounds were identified as citric acid, succinic acid, 3-hydroxy-3-methylglutaric acid (HMG), and 3,6,3',5'-tetramethoxy-5,7,4'-trihydroxyflavone-7-O-beta-D-g lucoside. The brine shrimp lethality of the acids was simply due to acidity; however, HMG elicited significant hypoglycemic responses in fasting normal mice. Ethyl and methyl esters of citric acid were prepared and tested in the murine hypoglycemic assay. Five of the predominant sugars were identified by tlc. Free thymidine was also isolated. Further evaluation of HMG and other potential inhibitors of HMG CoA lyase, in the treatment of symptoms of diabetes mellitus, is suggested.

show abstract

“…Moreover, two dicarboxylic acids, i.e., suberic and azelaic acid, were identified by their fragmentation patterns and showed good agreement with the reference substance fragmentation and t R . Cereals and whole grains are known to be a good source of carboxylic acids (Lohaus et al, 1983 ) and have been detected in BSG. However, as noted for the A extracts, some compounds remained unidentified.…”

Section: Resultsmentioning

confidence: 99%

Characterization of brewer's spent grain extracts by tandem mass spectrometry and HPLC‐DAD: Ferulic acid dehydrodimers, phenolamides, and oxylipins

Becker

Stegmüller

Richling

2022

Food Science & Nutrition

View full text Add to dashboard Cite

Brewer's spent grain (BSG) is a major by‐product of the brewing industry which is generated in high amounts. In recent years, sustainable food production has become more and more important. BSG mainly used as cattle feed has gained high interest due to not only its valuable ingredients such as fiber and proteins but also secondary metabolites remaining in BSG after the brewing process and known for many biological effects. In the present study, various methods were applied, such as acetone extraction (A), alkaline hydrolysis followed by ethyl acetate extraction (HE), and acetone extraction of alkaline hydrolysis residue (HA). Compounds present in the respective bioactive extracts were characterized by mass spectrometry to identify the active compounds. Various hydroxycinnamic acid derivatives as well as oxylipins and some dicarboxylic acids, such as azelaic acid, were present in HE and HA extracts. In contrast, some catechins and phenolamides, such as numerous hordatines, as well as oxylipins and phospholipids were detected in A extracts. Quantification using HPLC‐DAD revealed hordatine contents up to 172.2 ± 2.1 μg p‐ coumaric acid equivalents/mg extract. Hydroxycinnamic acid derivatives content accounted for up to 48% of the total extract (HE extracts) but only around 3% of the total HA extracts. In summary, all extracts contained secondary plant metabolites belonging to different classes, ranging from hydroxycinnamic acids to phenolamides, such as not only hordatines but also oxylipins, which were identified for the first time in BSG.

show abstract

Carboxylic Acids in Wheat, Rye and Barley

Cited by 8 publications

References 0 publications

Systemic Lactate Acts as a Metabolic Buffer in Humans and Prevents Nutrient Overflow in the Postprandial Phase

Systemic Lactate Acts as a Metabolic Buffer in Humans and Prevents Nutrient Overflow in the Postprandial Phase

Identification of 3-Hydroxy-3-methylglutaric Acid (HMG) as a Hypoglycemic Principle of Spanish Moss (Tillandsia usneoides)

Characterization of brewer's spent grain extracts by tandem mass spectrometry and HPLC‐DAD: Ferulic acid dehydrodimers, phenolamides, and oxylipins

Contact Info

Product

Resources

About