Carboxyl functionalized gold nanorods for sensitive visual detection of biomolecules

Scholz, Friedrich; Rüttinger, Lukas; Heckmann, Theresa; Freund, Lisa; Gad, Anne-Marie; Fischer, Tobias; Gütter, Andreas; Söffing, Hans Hermann

doi:10.1016/j.bios.2020.112324

Cited by 28 publications

(13 citation statements)

References 53 publications

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“…Colorimetric nanobiosensors, on the basis of the localized surface plasmon resonance (LSPR) phenomenon of noble metal nanomaterials, have recently drawn overwhelming attention. − There are four means of LSPR modulation: (1) aggregation, (2) growth kinetics, (3) metallization, and (4) etching of noble metal nanoparticles. Among various shapes of metal nanoparticles, such as nanorods, nanotriangles, and nanobipyramids, gold nanostars (AuNSs) are full of cutting-edge structures, which accumulate high-intensity electromagnetic fields.…”

Section: Introductionmentioning

confidence: 99%

Dual-Modal Immunosensor with Functionalized Gold Nanoparticles for Ultrasensitive Detection of Chloroacetamide Herbicides

Zha

et al. 2021

ACS Appl. Mater. Interfaces

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Convenient and ultrasensitive detection of pesticides is demanded for healthcare and environmental monitoring, which can be realized with a dual-modal strategy. In this paper, based on a biotin-labeled IgG-modified gold nanoparticle (AuNP@IgG-bio) probe, a dual-modal immunosensor was proposed for detecting chloroacetamide herbicides. This platform is relied on the dephosphorylation of ascorbic acid 2-phosphate (AA2P) by alkaline phosphatase (ALP). In addition to this process, ascorbic acid (AA)-triggered deposition of silver on gold nanostars (AuNSs) and the fluorogenic reaction of dehydrogenated AA and o-phenylenediamine (OPD) occur sequentially. Thus, the dual readout of the color change of red-green-blue (RGB) and fluorescence generation in situ induced by crystal growth can be used. The limits of detection (LODs) were as low as 1.20 ng/mL of acetochlor (ATC), 0.89 ng/mL of metolachlor, 1.22 ng/mL of propisochlor, and 0.99 ng/mL of their mixture by a smartphone and 0.44 ng/mL of ATC, 1.59 ng/mL of metolachlor, 2.80 ng/mL of propisochlor, and 0.72 ng/mL of their mixture by a spectrofluorometer. The recoveries from corn were 91.4–105.1% of the colorimetric mode and 92.4–106.2% of the fluorescent mode. Due to its simple observation mode and good performance, this dual-modal immunosensor possesses considerable application prospects.

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Section: Introductionmentioning

confidence: 99%

Dual-Modal Immunosensor with Functionalized Gold Nanoparticles for Ultrasensitive Detection of Chloroacetamide Herbicides

Zha

et al. 2021

ACS Appl. Mater. Interfaces

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“…After this preparation of the sensor surface, the actual CRP sensing step follows: 310 µg/ml CRP are ushed through the liquid cell, which leads to a measurable signal increase (13), which slightly decreases in a subsequent washing step (14), so that an overall CRP signal of about 0.2 nm results. Now, the already introduced secondary antibody is applied (15), testing on one hand the speci city of the CRP binding and, on the other hand, demonstrating possible signal ampli cation.…”

Section: Crp Detection Immobilization Via Thiolated Streptavidinmentioning

confidence: 99%

“…Several plasmonic nanoparticle-based methods are established in enzyme-linked immunosorbent assay (ELISA) platforms. These assays use labeled secondary antibodies in sandwich assays [12][13][14][15][16] or metal-enhanced optical signals by enzymatic deposition 15 or metal-enhanced uorescence 17 for the signal enhancement.…”

Section: Introductionmentioning

confidence: 99%

CRP Detection Using Gold Nanosensors – Effect of Layer Thickness and Immobilization Chemistry

Kastner¹,

Pritzke²,

Csáki³

et al. 2021

Preprint

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The immobilization of a capture molecule represents a crucial step for effective usage of gold nanoparticles in localized surface plasmon resonance (LSPR)-based bioanalytics. Depending on the immobilization method used, the resulting capture layer is of varying thickness. Thus, the target binding event takes place in different distances to the gold surface. Using the example of a C-reactive protein (CRP) immunoassay, different immobilization methods were tested and investigated with regard to their resulting target signal strength. The dependency of the target signal on the distance to the gold surface was investigated utilizing polyelectrolyte bilayers of different thickness. It could be experimentally demonstrated how much the LSPR-shift triggered by a binding event on the gold nanoparticles decreases with increasing distance to the gold surface. Thus, the sensitivity of an LSPR assay is influenced by the choice of immobilization chemistry.

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“…[18][19][20][21] Colloidal gold nanoparticles (GNPs), with diameters between 20 and 60 nm, have a maximum absorption peak at around 525 nm, which turns GNPs red in color and the colorimetric signal can be read by naked eyes. 22 In addition, proteins can easily combine with GNPs via hydrophobic and electrostatic interactions or covalent bonds. Due to the advantages of visibility, high stability and large surface area for conjugating biomolecules, GNPs have been widely used for LFIA.…”

Section: Introductionmentioning

confidence: 99%

“…To solve these problems, several other nanomaterials including gold nanorods (GNRs), quantum dots and up-conversion nanoparticles have been used as labeled probes in LFIA to obtain the uorescence or Raman signals, respectively. 4,7,22,26 However, the speed of data extraction is still an issue. Among these nanomaterials, GNRs demonstrate unique optical properties, good stability, excellent biocompatibility and higher extinction coefficient than spherical GNPs.…”

Section: Introductionmentioning

confidence: 99%

Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples

et al. 2021

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Carboxyl functionalized gold nanorods for sensitive visual detection of biomolecules

Cited by 28 publications

References 53 publications

Dual-Modal Immunosensor with Functionalized Gold Nanoparticles for Ultrasensitive Detection of Chloroacetamide Herbicides

Dual-Modal Immunosensor with Functionalized Gold Nanoparticles for Ultrasensitive Detection of Chloroacetamide Herbicides

CRP Detection Using Gold Nanosensors – Effect of Layer Thickness and Immobilization Chemistry

Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples

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