Carbosilane Dendrimers are a Non-Viral Delivery System for Antisense Oligonucleotides: Characterization of Dendriplexes

Pedziwiatr-Werbicka, Elzbieta; Shcharbin, Dzmitry; Malý, Jan; Malý, Marek; Zaborski, Marian; Gabara, Barbara; Ortega, Paula; Mata, F. Javier de la; Gómez, Rafael; Ma, Muñoz-Fernández; Klajnert, Barbara; Bryszewska, Maria

doi:10.1166/jbn.2012.1369

Cited by 34 publications

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“…[38] Studies on dendrimers both theoretical and computational aspects are going on nowadays expecting highly efficient gene delivery. [39] Carbosilane dendrimers for antisense oligonucleotides,[40] siRNA delivery using PAMAM[41] are also widely studied.…”

Section: Nonviral Gene Delivery Systemsmentioning

confidence: 99%

Novel gene delivery systems

Manjila

Baby

Bijin

et al. 2013

Int J Pharma Investig

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Gene therapy is an emerging field in medical and pharmaceutical sciences because of its potential in treating chronic diseases like cancer, viral infections, myocardial infarctions, and genetic disorders. Application of gene therapy is limited because of lack of suitable methods for proper introduction of genes into cells and therefore, this is an area of interest for most of the researchers. To achieve successful gene therapy, development of proper gene delivery systems could be one of the most important factors. Several nonviral and viral gene transfer methods have been developed. Even though the viral agents have a high transferring efficiency, they are difficult to handle due to their toxicity. To overcome the safety problems of the viral counterpart, several nonviral in vitro and in vivo gene delivery systems are developed. Out of these, the most promising and latest systems include polymer-based nonviral gene carriers, dendrimers, and physical means like electroporation, microinjection, etc., Shunning of possible immunogenicity and toxicity, and the feasibility of repeated administration are some of the merits of nonviral gene delivery systems over viral gene delivery. An ideal nonviral gene carrying system should possess all these merits without any compromise to its gene transferring efficiency. The viral gene delivery systems include lytic and nonlytic vectors for drug delivery. Inspite of its toxicity they are still preferred because of their long term expression, stability, and integrity. This review explores the recent developments and relevancy of the novel gene delivery systems in gene therapy.

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Section: Nonviral Gene Delivery Systemsmentioning

confidence: 99%

Novel gene delivery systems

Manjila

Baby

Bijin

et al. 2013

Int J Pharma Investig

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“…29,30 However, functionalization of the periphery with very polar moieties has turned them hydrophilic and thus suitable for biomedical applications. 31,[36][37][38][49][50][51][52][53][54][55][56] Thus, in this work we have used M1 macrophages as sensor cells to determine whether dendrimers can be detected by the organism as a foreign particle. 43,44 Others, have decorated the surface of carbosilane dendrimers with carbohydrates and studied their applications in biomedicine.…”

Section: Introductionmentioning

confidence: 99%

Study of cationic carbosilane dendrimers as potential activating stimuli in macrophages

et al. 2013

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Cationic carbosilane dendrimers with two ammonium groups per branch [G n O 3 (SiONN) m ] 2m+ (n ¼ 1, m ¼ 6; n ¼ 2, m ¼ 12; n ¼ 3, m ¼ 24), derived from the 1,3,5-trihydroxybenzene (1,3,5-C 6 H 3 (OH) 3 ) core, have been synthesized from reactions of dendrimers functionalized with Si-Cl bonds and the alcohol amineNMe 2 ) and further addition of MeI. The cationic dendrimers obtained were soluble in water, although they decomposed slowly in this medium. The toxicity and inflammatory activity of these dendrimers were evaluated in M1 macrophages and the results compared with the known carbosilane dendrimer 2G-NN16, derived from a Si atom core. The data showed good toxicity profiles for dendrimers [G n O 3 (SiONN) m ] 2m+ of first and second generation and 2G-NN16 and also that these dendrimers are not sensed as activating stimuli for macrophages and, as consequence, they do not cause an inflammatory response.

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“…[12][13][14][15][16][17][18][19][20][21][22] Furthermore, a number of polymeric nanoscale preparations have been used in clinical research, such as Genexol-PM ® , [23][24][25][26][27] NK105, 28 Nanoplatin ® , 29 etc. These nanoscale preparations belong to passive drug targeting delivery system, which can selectively extravasate and accumulate in tumor tissues for the result of the increased permeability of endothelial barriers in tumor blood vessels and the lack of effective lymphatic drainage from the tumor.…”

Section: Introductionmentioning

confidence: 99%

Synthesis, Characterization, and Application of Amino-Terminated Poly(ethylene glycol)–block–Poly(<I>ε</I>-caprolactone) Copolymer for Paclitaxel

Wang¹,

Liang²,

Deng³

et al. 2013

j. nanosci. nanotech.

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In this paper, we successfully synthesized amino-terminated poly(ethylene glycol)-block-poly (epsilon-caprolactone) (NH2-PEG-PCL) block copolymer from polyethylene glycol 2000, epsilon-caprolactone (epsilon-CL) and hydrazine hydrate. The obtained copolymer was characterized by nuclear magnetic resonance (1H-NMR), the molecular weight and distribution of NH2-PEG-PCL were characterized by Gel permeation chromatography (GPC). The NH2-PEG-PCL copolymer could self-assemble into micelles in water. Paclitaxel (PTX) loaded NH2-PEG-PCL (PNPP) micelles were prepared by solid dispersion technique without organic solvent. The micelles were characterized by XRD, TEM and Malvern laser particle size. The results of this work indicated that PNPP micelles were uniform and spherical shapes in solution. The average size and zeta potential of PNPP (DL = 8%) in water was about 97.1 +/- 1.2 nm, +13.9 +/- 0.6 mV, respectively. The in vitrodrug release profile of PNPP micelles showed a clear slow-release effect. The results suggested that NH2-PEG-PCL copolymer might be an excellent carrier for hydrophobic drugs such as PTX. In particular, the NH2-PEG-PCL polymer has potential value for modifying with ligands to work as active targeting drug delivery carriers, which has great significance for cancer therapeutics.

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Carbosilane Dendrimers are a Non-Viral Delivery System for Antisense Oligonucleotides: Characterization of Dendriplexes

Cited by 34 publications

References 0 publications

Novel gene delivery systems

Novel gene delivery systems

Study of cationic carbosilane dendrimers as potential activating stimuli in macrophages

Synthesis, Characterization, and Application of Amino-Terminated Poly(ethylene glycol)–block–Poly(<I>ε</I>-caprolactone) Copolymer for Paclitaxel

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