Carbonyl reductase mRNA abundance and enzymatic activity as potential biomarkers of oxidative stress in marine fish

Albertsson, Eva; Elhamirad, Amir Hossein; Sturve, Joachim; Larsson, D. G. Joakim; Förlin, Lars

doi:10.1016/j.marenvres.2012.07.001

Cited by 7 publications

(4 citation statements)

References 45 publications

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“…3f, i). Interestingly, cbr1 is involved in the metabolism of various fatty acids in the liver and has been associated with fatty acid-mediated cellular signalling in response to environmental perturbation 51 . As a final example, we highlight the cytotoxic effector perforin 1-like (prf1-like), an important player in liver-mediated energy balance and immune functions 52 .…”

Section: Resultsmentioning

confidence: 99%

Mapping epigenetic divergence in the massive radiation of Lake Malawi cichlid fishes

et al. 2021

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Epigenetic variation modulates gene expression and can be heritable. However, knowledge of the contribution of epigenetic divergence to adaptive diversification in nature remains limited. The massive evolutionary radiation of Lake Malawi cichlid fishes displaying extensive phenotypic diversity despite extremely low sequence divergence is an excellent system to study the epigenomic contribution to adaptation. Here, we present a comparative genome-wide methylome and transcriptome study, focussing on liver and muscle tissues in phenotypically divergent cichlid species. In both tissues we find substantial methylome divergence among species. Differentially methylated regions (DMR), enriched in evolutionary young transposons, are associated with transcription changes of ecologically-relevant genes related to energy expenditure and lipid metabolism, pointing to a link between dietary ecology and methylome divergence. Unexpectedly, half of all species-specific DMRs are shared across tissues and are enriched in developmental genes, likely reflecting distinct epigenetic developmental programmes. Our study reveals substantial methylome divergence in closely-related cichlid fishes and represents a resource to study the role of epigenetics in species diversification.

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Section: Resultsmentioning

confidence: 99%

Mapping epigenetic divergence in the massive radiation of Lake Malawi cichlid fishes

et al. 2021

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“…The role of CBR1 and CBR3 in the protection against ROS is underlined by their inducibility under conditions of oxidative stress (Ebert et al 2010 ; Miura et al 2013 ). In addition, toxic chemicals with impact on the environment like paraquat and even treated sewage are able to increase carbonyl reductase expression in Oncorhynchus mykiss and Zoarces viviparus (Albertsson et al 2010 , 2012 ).…”

Section: Introductionmentioning

confidence: 99%

The explosive trinitrotoluene (TNT) induces gene expression of carbonyl reductase in the blue mussel (Mytilus spp.): a new promising biomarker for sea dumped war relicts?

Strehse¹,

Brenner

Kisiela³

et al. 2020

Arch Toxicol

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Millions of tons of all kind of munitions, including mines, bombs and torpedoes have been dumped after World War II in the marine environment and do now pose a new threat to the seas worldwide. Beside the acute risk of unwanted detonation, there is a chronic risk of contamination, because the metal vessels corrode and the toxic and carcinogenic explosives (trinitrotoluene (TNT) and metabolites) leak into the environment. While the mechanism of toxicity and carcinogenicity of TNT and its derivatives occurs through its capability of inducing oxidative stress in the target biota, we had the idea if TNT can induce the gene expression of carbonyl reductase in blue mussels. Carbonyl reductases are members of the short-chain dehydrogenase/reductase (SDR) superfamily. They metabolize xenobiotics bearing carbonyl functions, but also endogenous signal molecules such as steroid hormones, prostaglandins, biogenic amines, as well as sugar and lipid peroxidation derived reactive carbonyls, the latter providing a defence mechanism against oxidative stress and reactive oxygen species (ROS). Here, we identified and cloned the gene coding for carbonyl reductase from the blue mussel Mytilus spp. by a bioinformatics approach. In both laboratory and field studies, we could show that TNT induces a strong and concentration-dependent induction of gene expression of carbonyl reductase in the blue mussel. Carbonyl reductase may thus serve as a biomarker for TNT exposure on a molecular level which is useful to detect TNT contaminations in the environment and to perform a risk assessment both for the ecosphere and the human seafood consumer.

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Section: The Explosive Trinitrotoluene (Tnt) Induces Gene Expression ...mentioning

confidence: 99%

“…The role of CBR1 and CBR3 in the protection against ROS is underlined by their inducibility under conditions of oxidative stress [50,51]. In addition, toxic chemicals with impact on the environment like paraquat and even treated sewage are able to increase carbonyl reductase expression in Oncorhynchus mykiss and Zoarces viviparus [52,53] In many biomonitoring studies, mussels are used as bioindicators for environmental pollution [54]. On the other hand, with regard to marine pollution by TNT and its metabolites, specific biomarkers are urgently sought to detect TNT contaminations in the environment and to perform a risk assessment both for the ecosphere and the human sea food consumer.…”

Section: The Explosive Trinitrotoluene (Tnt) Induces Gene Expression ...mentioning

confidence: 99%

Environmental and human toxicology studies on explosive chemicals leaking from submerged munitions

Maser,

Hartwig Bünning,

Strehse

2023

Propellants Explo Pyrotec

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This review provides an overview of 15 years of munitions research by the Institute of Toxicology and Pharmacology for Natural Scientists, University Medical School Schleswig‐Holstein, Kiel, Germany. As early as 2009, it was possible to detect the TNT metabolite 4‐ADNT in a quantity of 250 ng/g (wet weight) in blue mussels collected directly on munition items in the Kolberger Heide dumping area (Bight of Kiel, Baltic Sea, Germany). Based on these results, biomonitoring with blue mussels was established, in which uncontaminated mussels were exposed at certain distances from munition items and then retrieved after a few weeks in order to then be analyzed in the laboratory for energetic compounds (EC) including TNT and metabolites thereof. In following studies – in addition to mussels – also fish, sediments, water samples and passive samplers from specific locations were examined for the presence of EC. The study areas included dumping site regions from both the Baltic Sea and the North Sea, and were then extended to appropriate shipwrecks in the North Sea. The field studies were completed with laboratory investigations, which dealt with the development of a molecular biomarker as a kind of “early warning system” and with aspects of microbial degradation of EC. Finally, risk assessments for the marine environment and the human seafood consumer were carried out. The sections below are based on results from more than 10 scientific research projects and more than 20 publications in primary international research journals.

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Carbonyl reductase mRNA abundance and enzymatic activity as potential biomarkers of oxidative stress in marine fish

Cited by 7 publications

References 45 publications

Mapping epigenetic divergence in the massive radiation of Lake Malawi cichlid fishes

Mapping epigenetic divergence in the massive radiation of Lake Malawi cichlid fishes

The explosive trinitrotoluene (TNT) induces gene expression of carbonyl reductase in the blue mussel (Mytilus spp.): a new promising biomarker for sea dumped war relicts?

Environmental and human toxicology studies on explosive chemicals leaking from submerged munitions

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