Carbonic anhydrases CA1 and CA4 function in atmospheric CO2-modulated disease resistance

Zhou, Yeling; Vroegop-Vos, Irene A; Dijken, Anja J.H. Van; Does, Dieuwertje van der; Zipfel, Cyril; Pieterse, Corné M. J.; Wees, Saskia C.M. Van

doi:10.1007/s00425-020-03370-w

Cited by 21 publications

(18 citation statements)

References 77 publications

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“…However, Zhou et al. showed that, despite impaired stomatal closure preventing pathogen entry, PTI-mediated SA-dependent immunity against virulent P. syringae was enhanced in the βca1 βca4 double mutant ( Zhou et al., 2020 ). Furthermore, they revealed that the PRR-mediated downregulation of βCA1 and βCA4 expression was attenuated by high CO 2 .…”

Section: The Effects Of Carbon Dioxide Levels On Stomatal Immunitymentioning

confidence: 99%

Climate change impedes plant immunity mechanisms

Son

Park

2022

Front. Plant Sci.

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Rapid climate change caused by human activity is threatening global crop production and food security worldwide. In particular, the emergence of new infectious plant pathogens and the geographical expansion of plant disease incidence result in serious yield losses of major crops annually. Since climate change has accelerated recently and is expected to worsen in the future, we have reached an inflection point where comprehensive preparations to cope with the upcoming crisis can no longer be delayed. Development of new plant breeding technologies including site-directed nucleases offers the opportunity to mitigate the effects of the changing climate. Therefore, understanding the effects of climate change on plant innate immunity and identification of elite genes conferring disease resistance are crucial for the engineering of new crop cultivars and plant improvement strategies. Here, we summarize and discuss the effects of major environmental factors such as temperature, humidity, and carbon dioxide concentration on plant immunity systems. This review provides a strategy for securing crop-based nutrition against severe pathogen attacks in the era of climate change.

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Section: The Effects Of Carbon Dioxide Levels On Stomatal Immunitymentioning

confidence: 99%

Climate change impedes plant immunity mechanisms

Son

Park

2022

Front. Plant Sci.

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“…Plant β-CAs have been implicated in plant CO 2 metabolism, development, and host resistance ( Zhou et al, 2020 ). When potato CA-silenced lines were inoculated by using the oomycete Phytophthora infestans , potato late blight disease occurred seriously with the rapid growth and reproduction of P. infestans , indicating that suppression of CA increases susceptibility to the pathogen ( Restrepo et al, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Contribution of the Mitochondrial Carbonic Anhydrase (MoCA1) to Conidiogenesis and Pathogenesis in Magnaporthe oryzae

Dang

Batool

et al. 2022

Front. Microbiol.

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The interconversion of CO2 and HCO3− catalyzed by carbonic anhydrases (CAs) is a fundamental biochemical process in organisms. During mammalian–pathogen interaction, both host and pathogen CAs play vital roles in resistance and pathogenesis; during planta–pathogen interaction, however, plant CAs function in host resistance but whether pathogen CAs are involved in pathogenesis is unknown. Here, we biologically characterized the Magnaporthe oryzae CA (MoCA1). Through detecting the DsRED-tagged proteins, we observed the fusion MoCA1 in the mitochondria of M. oryzae. Together with the measurement of CA activity, we confirmed that MoCA1 is a mitochondrial zinc-binding CA. MoCA1 expression, upregulated with H2O2 or NaHCO3 treatment, also showed a drastic upregulation during conidiogenesis and pathogenesis. When MoCA1 was deleted, the mutant ΔMoCA1 was defective in conidiophore development and pathogenicity. 3,3′-Diaminobenzidine (DAB) staining indicated that more H2O2 accumulated in ΔMoCA1; accordingly, ATPase genes were downregulated and ATP content decreased in ΔMoCA1. Summarily, our data proved the involvement of the mitochondrial MoCA1 in conidiogenesis and pathogenesis in the rice blast fungus. Considering the previously reported HCO3− transporter MoAE4, we propose that MoCA1 in cooperation with MoAE4 constitutes a HCO3− homeostasis-mediated disease pathway, in which MoCA1 and MoAE4 can be a drug target for disease control.

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“…The spatial correlation between gene expression and microbial abundance is well illustrated by the expression patterns of three genes, ACD6 , CA1 and LURP1 ( Figure 4d , Supplementary Figure S24 ). All three genes are related to the basal plant immunity– ACD6 is a broad-spectrum disease resistance gene activated by diverse microbes (Lu et al, 2003), the CA1 gene product binds the immune-related hormone salicylic acid (Poque et al, 2018) and regulates stomatal opening during pathogen invasions (Zhou et al, 2020), and LURP1 is required for resistance to the pathogen Hyaloperonospora parasitica (Knoth and Eulgem, 2008). Overall, these results reveal a connection between the spatial organization of microbes within the leaf and the host expression signature.…”

Section: Resultsmentioning

confidence: 99%

Spatially resolved host-bacteria-fungi interactomes via spatial metatranscriptomics

Saarenpää

Shalev

Ashkenazy

et al. 2022

Preprint

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All multicellular organisms are closely associated with microbes, which have a major impact on the health of their host. The interactions of microbes among themselves and with the host take place at the microscale, forming complex networks and spatial patterns that are rarely well understood due to the lack of suitable analytical methods. The importance of high-resolution spatial molecular information has become widely appreciated with the recent advent of spatially resolved transcriptomics. Here, we present Spatial metaTranscriptomics (SmT), a sequencing-based approach that leverages 16S/18S/ITS/poly-d(T) multimodal arrays for simultaneous host transcriptome- and microbiome-wide characterization of tissues at 55-µm resolution. We showcase SmT in outdoor-grown Arabidopsis thaliana leaves as a model system, and found tissue-scale bacterial and fungal hotspots. By network analysis, we study inter- and intra-kingdom spatial interactions among microbes, as well as the host response to microbial hotspots. SmT is a powerful new strategy that will be pivotal to answering fundamental questions on host-microbiome interplay.

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Carbonic anhydrases CA1 and CA4 function in atmospheric CO2-modulated disease resistance

Cited by 21 publications

References 77 publications

Climate change impedes plant immunity mechanisms

Climate change impedes plant immunity mechanisms

Contribution of the Mitochondrial Carbonic Anhydrase (MoCA1) to Conidiogenesis and Pathogenesis in Magnaporthe oryzae

Spatially resolved host-bacteria-fungi interactomes via spatial metatranscriptomics

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