Carbonic Anhydrase of the Alkaliphilic Cyanobacterium Microcoleus chthonoplastes

Kupriyanova, Elena V.; Markelova, A. G.; Лебедева, Н. В.; Герасименко, Л. М.; Zavarzin, G. A.; Пронина, Н. А.

doi:10.1023/b:mici.0000032233.36705.5a

Cited by 8 publications

(7 citation statements)

References 10 publications

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“…Our findings in the heterologous Synechocystis test system contrast sharply with the proposed location of CahB1 outside of the Sodalinema gerasimenkoae carboxysome and outside the cytosol altogether [25,26]. While no extracellular CA activity could be observed upon expression of CahB1 in Synechocystis, a slight yet statistically significant enhancement of CA activity in the carboxysome-enriched cell fraction P I of WT + cahB1 cells as compared to the parental WT cells (Figure 3d) may account for the enhanced growth observed in the said expression strains (Figure 2b).…”

Section: Discussioncontrasting

confidence: 99%

“…Meanwhile, for the entire measuring period of five minutes, no elevation above the uncatalyzed background rates was observed in intact cells assayed for extracellular CA activity (Figure 3d left) or acetazolamide-treated P I fraction samples (Figure 3d right). These findings suggest that in Synechocystis CahB1 localizes to a cellular subfraction that harbours carboxysomes, and that the enzymatic activity of CahB1 is restricted to the said fraction rather than to the cell surface as would be expected based on previous reports from Sodalinema gerasimenkoae [24][25][26].…”

Section: Cahb1 Localizes To the Carboxysome In Synechocystissupporting

confidence: 79%

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Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803

Minagawa

Dann

2023

Plants

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Cyanobacteria mostly rely on the active uptake of hydrated CO2 (i.e., bicarbonate ions) from the surrounding media to fuel their inorganic carbon assimilation. The dehydration of bicarbonate in close vicinity of RuBisCO is achieved through the activity of carboxysomal carbonic anhydrase (CA) enzymes. Simultaneously, many cyanobacterial genomes encode extracellular α- and β-class CAs (EcaA, EcaB) whose exact physiological role remains largely unknown. To date, the CahB1 enzyme of Sodalinema gerasimenkoae (formerly Microcoleus/Coleofasciculus chthonoplastes) remains the sole described active extracellular β-CA in cyanobacteria, but its molecular features strongly suggest it to be a carboxysomal rather than a secreted protein. Upon expression of CahB1 in Synechocystis sp. PCC6803, we found that its expression complemented the loss of endogenous CcaA. Moreover, CahB1 was found to localize to a carboxysome-harboring and CA-active cell fraction. Our data suggest that CahB1 retains all crucial properties of a cellular carboxysomal CA and that the secretion mechanism and/or the machinations of the Sodalinema gerasimenkoae carboxysome are different from those of Synechocystis.

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Section: Discussioncontrasting

confidence: 99%

Section: Cahb1 Localizes To the Carboxysome In Synechocystissupporting

confidence: 79%

Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803

Minagawa

Dann

2023

Plants

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“…Further, EcaA enzymes have been found in other cyanobacteria, such as the alkalophilic cyanobacteria Rhabdoderma lineare and Microcoleus chthonoplates , and the marine cyanobacterium Cyanothece sp. ATCC51142 [ 104 , 105 , 106 ]. Cyanothece EcaA is a highly active CA, whose enzymatic activity was confirmed both in vivo and in vitro , using intact cells and a recombinant protein expressed in a heterologous host, respectively [ 106 ].…”

Section: Cyanobacteriamentioning

confidence: 99%

“…Finally, EcaAs isolated from R. lineare and M. chthonoplates are two extracellular α-CAs, localized in the glycocalyx membrane [ 105 , 108 ], probably involved in cell survival under extreme conditions of soda lakes. Indeed, it has been suggested that these enzymes may preserve the intracellular Ci pool for photoautotrophic assimilation, preventing CO 2 leakage from the cell through its conversion into bicarbonate [ 99 , 109 ].…”

Section: Cyanobacteriamentioning

confidence: 99%

α-CAs from Photosynthetic Organisms

Langella

Fiore

Alterio

et al. 2022

IJMS

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Carbonic anhydrases (CAs) are ubiquitous enzymes that catalyze the reversible carbon dioxide hydration reaction. Among the eight different CA classes existing in nature, the α-class is the largest one being present in animals, bacteria, protozoa, fungi, and photosynthetic organisms. Although many studies have been reported on these enzymes, few functional, biochemical, and structural data are currently available on α-CAs isolated from photosynthetic organisms. Here, we give an overview of the most recent literature on the topic. In higher plants, these enzymes are engaged in both supplying CO2 at the Rubisco and determining proton concentration in PSII membranes, while in algae and cyanobacteria they are involved in carbon-concentrating mechanism (CCM), photosynthetic reactions and in detecting or signaling changes in the CO2 level in the environment. Crystal structures are only available for three algal α-CAs, thus not allowing to associate specific structural features to cellular localizations or physiological roles. Therefore, further studies on α-CAs from photosynthetic organisms are strongly needed to provide insights into their structure–function relationship.

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“…A high concentration of carbonate ions (due to a high partial pressure of carbon dioxide at that time) and a low concentration of calcium ions would promote calcium carbonate precipitation far from cells which consequently would not get encrusted, and would be thus not preserved as microfossils. Other authors argue that biological parameters such as sheath EPS composition (Dupraz and Visscher, 2005;Obst et al, 2009) and/or cell activity (Kupriyanova et al, 2011) being additional critical parameters, ancestral cyanobacteria may have not been able to provoke extracellular carbonate nucleation and precipitation efficiently (Jansson and Northen, 2010;Couradeau et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Cyanobacterial calcification in modern microbialites at the submicrometer-scale

Couradeau¹,

Benzerara²,

Gérard³

et al. 2013

Preprint

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The search for microfossils in the geological record has been a long-term challenge. Part of the problem comes from the difficulty of identifying such microfossils unambiguously, since they can be morphologically confused with abiotic biomorphs. One route to improve our ability to correctly identify microfossils consists in studying fossilization processes affecting bacteria in modern settings. We studied the initial stages of fossilization of cyanobacterial cells in modern microbialites from Lake Alchichica (Mexico), a Mg-rich hyperalkaline crater lake (pH 8.9) hosting currently growing stromatolites composed of aragonite [CaCO3] and hydromagnesite [Mg5(CO3)4(OH)2 × 4(H2O)]. Most of the biomass associated with the microbialites is composed of cyanobacteria. Scanning electron microscopy analyses coupled with confocal laser scanning microscopy observations were conducted to co-localize cyanobacterial cells and associated minerals. These observations showed that cyanobacterial cells affiliating to the order Pleurocapsales become specifically encrusted within aragonite with an apparent preservation of cell morphology. Encrustation gradients from non-encrusted to totally encrusted cells spanning distances of a few hundred micrometers were observed. Cells exhibiting increased levels of encrustation along this gradient were studied down to the nm-scale using a combination of focused ion beam (FIB) milling, transmission electron microscopy (TEM) and scanning transmission X-ray microscopy (STXM) at the C, O and N K-edges. Two different types of aragonite crystals were observed: one type was composed of needle-shaped nano-crystals growing outward from the cell body with a crystallographic orientation perpendicular to the cell wall, and another type was composed of larger crystals that progressively filled the cell interior. Organic matter (OM), initially co-localized with the cell, decreased in concentration and dispersed away from the cell while crystal growth occurred. As encrustation developed, OM progressively disappeared, but remaining OM showed the same spectroscopic signature. In the most advanced stages of fossilization, only the textural organization of the two types of aragonite recorded the initial cell morphology and spatial distribution

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Carbonic Anhydrase of the Alkaliphilic Cyanobacterium Microcoleus chthonoplastes

Cited by 8 publications

References 10 publications

Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803

Extracellular CahB1 from Sodalinema gerasimenkoae IPPAS B-353 Acts as a Functional Carboxysomal β-Carbonic Anhydrase in Synechocystis sp. PCC6803

α-CAs from Photosynthetic Organisms

Cyanobacterial calcification in modern microbialites at the submicrometer-scale

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