Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems

Chen, Liping; Chen, Hang; Hu, Zekun; Tian, Yucheng; Wang, Cenchao; Xie, Peiran; Deng, Xuhan; Zhang, Yushen; Tang, Xia; Lin, Xueran; Li, Biqing; Wei, Chaohai; Qiu, Guofu

doi:10.1016/j.watres.2022.118258

Cited by 49 publications

(12 citation statements)

References 73 publications

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“…This study also demonstrated that clade 1–4 possess genes encoding enzymes related to glycogen synthesis ( galU ) and degradation ( glgP ). In other studies, glycogen synthesis in Tetrasphaera-PAOs was detected with glucose, aspartic acid, and glutamic acid as carbon sources, which was suggested to play a key role in phosphate removal. ,, …”

Section: Resultsmentioning

confidence: 81%

“…In other studies, glycogen synthesis in Tetrasphaera-PAOs was detected with glucose, aspartic acid, and glutamic acid as carbon sources, 45 which was suggested to play a key role in phosphate removal. 12,13,18 Almost all Tetrasphaera-MAGs encode the low-affinity phosphate transporter protein Pit and the high-affinity phosphate transporter proteins PstSCAB. Other genes associated with poly-P synthesis and hydrolysis including ppk and ppk2 were also present.…”

Section: Resultsmentioning

confidence: 99%

“…Biological phosphorus removal is more cost-effective and sustainable than chemical phosphorus removal in wastewater treatment. , Polyphosphate-accumulating organisms (PAOs) use polyphosphate (poly-P) as an energy source under carbon-rich anaerobic (feast) condition and convert carbon sources into biomacromolecules such as polyhydroxyalkanoates (PHA). Under the subsequent carbon-deficient aerobic (famine) condition, phosphate is accumulated as poly-P. − Organic carbon source is a key factor influencing P release and uptake activities. , The classical PAO model was established with Candidatus Accumulibacter, where volatile fatty acids (VFAs) served as carbon sources. , Fermentative PAOs such as Tetrasphaera were studied more recently, which can hydrolyze complex substrates including amino acids and sugars and are not limited to the circulating glycogen and PHA to gain energy for growth. − In many wastewater treatment plants (WWTPs), Tetrasphaera (1.3–11.9%) can be more abundant than Ca . Accumulibacter (0–2.9%), indicating their potentially overlooked roles in phosphorus removal …”

Section: Introductionmentioning

confidence: 99%

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Phosphorus Removal and Storage Polymer Synthesis by Tetrasphaera-Related Bacteria with Different Carbon Sources

Pei-liang

et al. 2023

ACS EST Water

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Tetrasphaera are polyphosphate-accumulating organisms (PAOs) prevalent in wastewater phosphorus removal systems. However, their metabolic versatility on carbon sources and correlations between carbon and phosphorus metabolism have not been fully elucidated. In this study, we identified a new clade of Tetrasphaera as the dominant PAO in a lab-scale phosphorus removal reactor. Both phosphorus release and uptake by Tetrasphaera-PAOs were more efficient with acetate than with glycerol and glucose. Fermentation of glycerol and glucose provided 4 and 1.7 times more energy than poly-P hydrolysis, and the trade-off between the two processes resulted in poor phosphorus release. Raman-fluorescence in situ hybridization suggested that polyhydroxyalkanoates (PHA) were synthesized from acetate. Glycogen, on the other hand, was the key energy storage compound formed using glycerol and glucose. Aerobic phosphorus uptake with acetate was 4.4 and 1.8 times more efficient than glycerol and glucose due to the additional energy supplied by PHA hydrolysis. The metabolic versatility and fermentation capability of Tetrasphaera-PAOs supported their prevalence in EBPR and underlined their potential metabolic interactions with other populations in the community. This work adds to our understanding of the genetic and metabolic divergence of Tetrasphaera-PAOs, providing useful information for the optimization of wastewater phosphorus removal under varied carbon source conditions.

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Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phosphorus Removal and Storage Polymer Synthesis by Tetrasphaera-Related Bacteria with Different Carbon Sources

Pei-liang

et al. 2023

ACS EST Water

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“…PAOs release stored polyphosphate in the form of PO43-to obtain energy. In aerobic conditions, PAOs oxidize and decompose the organic substances stored in their bodies, producing energy and absorbing excess phosphorus from the wastewater, storing it in polyphosphate [20]. As a result, the discharged residual sludge contains a large amount of phosphorus, achieving the goal of phosphorus removal.…”

Section: Mbr Application In A2/o Tank Of Wwtp In Shanghaimentioning

confidence: 99%

The recent application of bioreactor membrane (MBR) in China: for industrial sewage treatment in WWTP and its features

Zhang

2023

International Conference on Modern Medicine and Global Health (ICMMGH 2023)

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“…Phosphorus (P) is a pollutant in surface waters and also a non-renewable resource (Oehmen et al, 2007). Enhanced biological phosphorous removal (EBPR) is a widely applied process in municipal wastewater treatment plants (WWTPs) (Chen et al, 2022; Mino et al, 1998); it requires polyphosphate accumulating organisms (PAOs) capable of releasing P under anaerobic, and taking up more P than normally required for metabolism and growth, under aerobic conditions (He and McMahon, 2011; Lu et al, 2021; Zhang et al, 2022). Candidatus Accumulibacter represents a key group of PAOs in lab- and full-scale EBPR systems (Martín et al, 2006; Petriglieri et al, 2022; Qiu et al, 2020; Singleton et al, 2022; Srinivasan et al, 2021; Tomás-Martínez et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Cas phage defense systems and prophages inCandidatusAccumulibacter

Deng

Yuan

Chen

et al. 2022

Preprint

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Candidatus Accumulibacter is a key genus of polyphosphate-accumulating organisms (PAOs) found in laboratory- and full-scale wastewater treatment systems, mediating enhanced biological phosphorus removal (EBPR). However, little is known about their ability to resist phage infection. We conducted a systematic analysis of the occurrence and characteristics of clustered regularly interspaced short palindromic repeats and associated proteins (CRISPR-Cas) systems and prophages in diverse Ca. Accumulibacter taxa (43 in total, including 10 newly recovered genomes). Fourty complete CRISPR loci were identified in 28 genomes, falling into 6 subtypes. The occurrence and distribution of CRISPR-Cas systems did not follow a vertical evolutionary relationship. Phylogenetic analyses of the cas genes and direct repeats (DRs) suggested that the CRISPR-Cas systems were likely acquired via horizontal gene transfer, with acquisition rates higher than those of speciation, rendering different Ca. Accumulibacter distinct adaptivity to phage predations. 2448 spacers were identified, 67 of them matched to known phages. Major differences were observed among the numbers of spacers for different Ca. Accumulibacter, showing unique phages that could be resisted by different members. A comparison of the spacers in genomes having the same cas gene but from distinct geographical locations indicated that habitat isolation may have resulted in the acquisition of different spacers by different Ca. Accumulibacter. Metagenomic analysis allowed the identification of 26 viral contigs (18 are Caudovirales members) in 6 metagenomic datasets from three lab-scale enrichment reactors, matching to 73 spacers in 10 Ca. Accumulibacter genomes in these reactors, showing the specific immunity of these Ca. Accumulibacter. Metatranscriptomic analyses showed the activity of the CRISPR-Cas system under both anaerobic and aerobic conditions. Extra efforts were made to identify prophages in Ca. Accumulibacter genomes. In total, 133 prophage regions were identified. Twenty-seven of them were classified as potentially active prophages. Three prophages (all are Caudovirales members, in DS2011, SCELSE-7IIH and SCELSE-5IIH, respectively) are readily activable. Differences in the occurrence of CRISPR-Cas systems and prophages in Ca. Accumulibacter will lead to their distinct responses under phage predation. This study represents the first systematic analysis of CRISPR-Cas systems and prophages with combined experimental and bioinformatic methods in the Ca. Accumulibacter lineage, providing new perspectives on the potential impacts of phages on Ca. Accumulibacter and EBPR systems.

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Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems

Cited by 49 publications

References 73 publications

Phosphorus Removal and Storage Polymer Synthesis by Tetrasphaera-Related Bacteria with Different Carbon Sources

Phosphorus Removal and Storage Polymer Synthesis by Tetrasphaera-Related Bacteria with Different Carbon Sources

The recent application of bioreactor membrane (MBR) in China: for industrial sewage treatment in WWTP and its features

CRISPR-Cas phage defense systems and prophages inCandidatusAccumulibacter

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