Carbon substrate re‐orders relative growth of a bacterium using Mo‐, V‐, or Fe‐nitrogenase for nitrogen fixation

Luxem, Katja E.; Kraepiel, Anne M. L.; Zhang, Lichun; Waldbauer, Jacob R.; Zhang, Xinning

doi:10.1111/1462-2920.16001

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…One of the most intensively studied PNSB is R. palustris CGA009 (8), yet little is known about its ability to respire anaerobically. Unlike some other model PNSB, CGA009 cannot grow via respiration with dimethylsulfoxide (9-11). However, a partial denitrification pathway was identified in the CGA009 genome (8).…”

Section: Resultsmentioning

confidence: 99%

“…R. palustris is one of the most metabolically versatile PNSB (13), yet little is known about its ability to respire anaerobically. Unlike some other model PNSB, CGA009, and its derivative CGA0092 (14) used herein, cannot grow via respiration with dimethylsulfoxide (15)(16)(17). However, according to its genome sequence, it should be capable of partial denitrification, as it has putative enzymes for converting NO2to N2 (10, 13) (Fig.…”

Section: Introductionmentioning

confidence: 99%

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Nitrous oxide reduction by two partial denitrifying bacteria requires denitrification intermediates that cannot be respired

LaSarre

Morlen

Neumann

et al. 2022

Preprint

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Denitrification is a form of anaerobic respiration wherein nitrate is sequentially reduced via nitrite, nitric oxide, and nitrous oxide to dinitrogen gas. The pathway has mostly been characterized in bacteria that have the complete denitrification pathway. However, each individual step can serve as a form of anaerobic respiration and many bacteria only have partial denitrification pathways. The conditions under which these partial denitrification pathways are activated have received comparatively little attention. Here we report the activity of partial denitrification pathways in two purple nonsulfur bacteria, Rhodopseudomonas palustris CGA009 and Rhodobacter capsulatus SB1003. These bacteria can use one or more denitrification steps as an electron sink during phototrophic growth or to transform energy during anaerobic respiration in the dark. In each case, nitrous oxide reduction required supplementation with a non-catalyzable denitrification intermediate. Thus, bacteria that maintain partial denitrification pathways are still subject to regulation by denitrification intermediates that they cannot use.ImportanceDenitrification is a form of microbial respiration wherein nitrate is converted into dinitrogen gas, a major component of Earth’s atmosphere, via several nitrogen oxide intermediates. Unfortunately, denitrification of nitrate in agricultural fertilizers is often incomplete, resulting in the emission of the potent greenhouse gas, nitrous oxide. Some bacteria do not have all the steps for denitrification, but many can still reduce nitrous oxide into harmless dinitrogen gas. These partial denitrifying bacteria have received little attention. Here we characterized two partial denitrifying bacteria that are capable of nitrous oxide reduction. Surprisingly, nitrous oxide reduction was induced by denitrification intermediates that the bacteria could not respire, suggesting that regulation of nitrous oxide reduction was subject to the same cues as in complete denitrifiers. This work thus informs on how partial denitrifying bacteria can contribute to, and potentially combat, greenhouse gas emissions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nitrous oxide reduction by two partial denitrifying bacteria requires denitrification intermediates that cannot be respired

LaSarre

Morlen

Neumann

et al. 2022

Preprint

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“…Since only a subset of diazotrophs encodes alternative nitrogenases, it has been suggested these enzymes merely provide a backup function in molybdenum‐limiting environments. However, recent studies have demonstrated that the vanadium enzyme can provide faster growth than the Mo nitrogenase in vivo, dependent on the nature of the carbon source (Luxem et al, 2020) and the Fe‐only enzyme has the unique ability to catalyse the combined reduction of carbon dioxide and dinitrogen to produce significant levels of methane in addition to ammonia, suggesting a potential role for this enzyme in shaping microbial community interactions in marine environments (Zheng et al, 2018). These discoveries in combination with nitrogenase activity measurements in various ecosystems (Bellenger et al, 2014; Darnajoux et al, 2019; McRose et al, 2017) suggest that alternative nitrogenases may provide critical functionality in some environmental niches (Harwood, 2020).…”

Section: Introductionmentioning

confidence: 99%

Interactions between paralogous bacterial enhancer‐binding proteins enable metal‐dependent regulation of alternative nitrogenases in Azotobacter vinelandii

Appia-Ayme

Little

Chandra

et al. 2022

Molecular Microbiology

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The ability to carry out biological nitrogen fixation, a key process in the nitrogen cycle that underpins sustainable agriculture, is conferred in diverse bacteria and archaea by nitrogenase enzymes that catalyse the reduction of gaseous dinitrogen to ammonia. All diazotrophs that have been sequenced so far encode molybdenum nitrogenase with an active site co-factor designated as FeMo-co that contains a complex inorganic cluster [7Fe-9S-C-Mo] in which the molybdenum atom is bound to a molecule of R-homocitrate (Burén et al., 2020;Einsle & Rees, 2020). However, in addition to expressing the molybdenum nitrogenase enzyme, some diazotrophs also

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Dehazing redox homeostasis to foster purple bacteria biotechnology

Alloul¹,

Blansaer²,

Segura³

et al. 2023

Trends in Biotechnology

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Carbon substrate re‐orders relative growth of a bacterium using Mo‐, V‐, or Fe‐nitrogenase for nitrogen fixation

Cited by 4 publications

References 34 publications

Nitrous oxide reduction by two partial denitrifying bacteria requires denitrification intermediates that cannot be respired

Nitrous oxide reduction by two partial denitrifying bacteria requires denitrification intermediates that cannot be respired

Interactions between paralogous bacterial enhancer‐binding proteins enable metal‐dependent regulation of alternative nitrogenases in Azotobacter vinelandii

Dehazing redox homeostasis to foster purple bacteria biotechnology

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