Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides

Achimón, Fernanda; Dambolena, José Sebastián; Zygadlo, Julio Alberto; Pizzolitto, Romina Paola

doi:10.1016/j.lwt.2019.108470

Cited by 14 publications

(9 citation statements)

References 43 publications

(41 reference statements)

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“…The collection of VOCs was performed from the 5th to the 7th day of incubation, as previous studies detected maximum VOC production in this time frame with other species of Fusarium [25]. A closed-loop system, as described by Achimón et al [21], was used. Briefly, each 100-mL flask was closed with a rubber stopper with a gas inlet and a gas outlet coupled to a trap.…”

Section: Collection and Identification Of Vocsmentioning

confidence: 99%

“…It is well-known that the biosynthesis of VOCs by filamentous fungi proceeds through different primary and secondary metabolic pathways [1], where the metabolism of fatty acids plays a prevalent role. A handful of VOCs derived from lipid peroxidation (i.e., 3-octanol, 3-octanone, 1-octen-3-ol, and 1-octen-3-one) and the terpene profile of F. verticillioides have been evaluated under different culture conditions [4,[19][20][21][22]; however, most of the hydrocarbon-derived VOCs emitted by F. verticillioides remain unexplored.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides

Achimón

Brito

Pizzolitto

et al. 2022

JoF

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The aim of the present study was to evaluate the effect of different carbon sources on the hydrocarbon-like volatile organic compounds (VOCs) of Fusarium verticillioides strain 7600 through a Principal Component Analysis approach, and to explore their diesel potential by using data from the literature. The fungus was cultivated in GYAM culture medium, and five carbon sources were evaluated: glucose, sucrose, xylose, lactose, and fructose. The VOCs were collected using a close-loop apparatus and identified through GC-MS. The same profile of 81 VOCs was detected with all treatments, but with different relative percentages among carbon sources. The production of branched-chain alkanes (30 compounds) ranged from 25.80% to 38.64%, straight-chain alkanes (12 compounds) from 22.04% to 24.18%, benzene derivatives (12 compounds) from 7.48% to 35.58%, and the biosynthesis of branched-chain alcohols (11 compounds) was from 6.82% to 16.71%, with lower values for the remaining groups of VOCs. Our results show that F. verticillioides has the metabolic potential to synthesize diesel-like VOCs. Further research should include the optimization of culture conditions other than carbon sources to increase the production of certain groups of VOCs.

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Section: Collection and Identification Of Vocsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides

Achimón

Brito

Pizzolitto

et al. 2022

JoF

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“…Diluted samples (1/100 v/v in n-heptane) of 1 µL of each EO were manually injected using the splitless mode. Kovats retention indices (KI) were calculated after an analysis of C8-C21 alkane series (Sigma-Aldrich), under the same chromatographic conditions (Achimón et al, 2019). The identification of EO compounds was based on the comparison of their KI and mass spectra with those from the NIST-08 Mass Spectral Library (US National Institute of Standards and Technology; Stein et al, 2008) and literature data (Achimón, Krapacher et al, 2021).…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms) Analysesmentioning

confidence: 99%

Insecticidal and antifungal effects of lemon, orange, and grapefruit peel essential oils from Argentina

Achimón

Leal

Pizzolitto

et al. 2022

AgriS

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The aim of the present work was to study the bioactivity of lemon, orange, and grapefruit peel essential oils (EOs) obtained from Argentinian plantations on different agronomically important insect and fungal species. The chemical profile of EOs was determined by Gas Chromatography-Mass Spectrometry (GC/MS); the insecticidal activity was studied through contact and fumigant assays; the antifungal activity was evaluated with fumigant tests. Orange EO was the most effective against Rhyzopertha dominica, Oryzaephilus spp. and Sitophilus granarius in fumigation tests (LC50= 89.39, 94.50, and 163.64 µL/L air, respectively); while the insecticidal effect of EOs was species-dependent in contact toxicity assays. Regarding antifungal activity, Rhizoctonia solanii was more susceptible than Sclerotium rolfsii to the tested EOs, with lemon from Industry 1 and orange being the most toxic EOs (MIC=10.77 µL/plate and 11.02 µL/plate, respectively). Conversely, S. rolfsii was strongly inhibited by lemon EO from Industry 2 (MIC= 52.40 µL/plate). Besides limonene, other compounds that could be responsible for these bioactivities were: linalool, carvone, α-pinene, β-pinene, β-myrcene, α-terpineol, terpinen-4-ol, limonene oxide, β-phellandrene, γ-terpinene, sabinene, neral, neryl acetate, β-caryophyllene and p-cymene. Citrus peel EOs can be used against different pests, contributing to the valorization of citrus residues.

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“…The aim of this study was to assess how the production of phenolic compounds by Euglena gracilis cultures is influenced by the carbon source (i.e., glutamic acid, malic acid, glucose or reduced glutathione). Earlier studies [ 30 , 31 , 32 , 33 ] have shown the direct and important impact of growth conditions, as variable organic carbon sources have an impact on secondary metabolite production. The most common analytical method for the quantification and characterization of phenolic compounds is UV-visible spectrophotometry using the Folin–Ciocalteu (FC) reagent method [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of Carbon Sources on the Phenolic Compound Production by Euglena gracilis Using an Untargeted Metabolomic Approach

Bernard

Guéguen

2022

Biomolecules

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Industrial development and urbanization has led to the diverse presence of metals in wastewater that are often improperly treated. The microalgae Euglena gracilis can tolerate high concentrations of metal via the excretion of organic metabolites, including phenolics. This study aims to evaluate how carbon amendment stimulates phenolic compound production by E. gracilis. The number, relative intensity and molecular composition of the phenolic compounds were significantly different between each of four carbon amended cultures (i.e., glutamic acid, malic acid, glucose, reduced glutathione) during the log phase. Phenolic compounds were mainly produced during the minimum growth rate, likely a response to stressful conditions. A better understanding of phenolic compounds production by E. gracilis and the impact of growth conditions will help identify conditions that favor certain phenolic compounds for dietary and metal chelation applications.

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Carbon sources as factors affecting the secondary metabolism of the maize pathogen Fusarium verticillioides

Cited by 14 publications

References 43 publications

Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides

Effect of Carbon Sources on the Production of Volatile Organic Compounds by Fusarium verticillioides

Insecticidal and antifungal effects of lemon, orange, and grapefruit peel essential oils from Argentina

Influence of Carbon Sources on the Phenolic Compound Production by Euglena gracilis Using an Untargeted Metabolomic Approach

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