Carbon source regulation of β-galactosidase biosynthesis inPenicillium chrysogenum

Nagy, Zoltán; Keresztessy, Zsolt; Szentirmai, Attila; Bíró, Sándor

doi:10.1002/1521-4028(200112)41:6<351::aid-jobm351>3.0.co;2-o

Cited by 16 publications

(7 citation statements)

References 46 publications

(39 reference statements)

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“…Most studies on suitable substrates for penicillium fermentation had centred on the use of refined substrates such as glucose, sucrose, glycerol, galactose and lactose [28] . The present result indicates that cassava peels are a good substrate for natural penicillin production by P. chrysogenum PCL501.…”

Section: Discussionmentioning

confidence: 99%

Physiological effect of Natural Penicillin Extract on Cassava Peel Media by Penicillium chrysogenum PCL501 on E.coli Infected Wistar Rats

Onyegeme-Okerenta¹,

Ebuehi²

2017

IOSRPHR

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ABSTRACT:-Some physiological effect of natural penicillin extract produced on cassava peel media by Penicillium chrysogenum PCL501 on E.coli infected Wistar rats were evaluated. Fermentation of P. chrysogenum PCL501 with cassava peels -an agro-waste-produced natural antibiotics. In vitro antibiotic activity of cassava peels culture extract was tested against two clinical bacterial isolates, namely, Bacillus subtilis, and Escherichia coli. The culture extract and standard drug (commercial Benzyl Penicillin) inhibited the growth B. subtilis and E. coli. Antibiotic activity of the culture extract was comparable with that of the standard drug. In vivo antibiotic action on Wistar Wistar rats infected with E. coli showed that the penicillin produced on cassava peel was potent as its administration leads to the recovery of the infected animals. Evaluation of biochemical indices of E. coli infected Wistar rats and treatment with extract confirms that the extract is a potent antibiotic. The haematological evaluation showed a significant decrease (p<0.05) in platelet count in animals administered with both the extract and reference drug. Cassava peels are indicated as a suitable and cheap carbon source for the production of penicillin by P. chrysogenum PCL501.

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Section: Discussionmentioning

confidence: 99%

Physiological effect of Natural Penicillin Extract on Cassava Peel Media by Penicillium chrysogenum PCL501 on E.coli Infected Wistar Rats

Onyegeme-Okerenta¹,

Ebuehi²

2017

IOSRPHR

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“…In this work, the growth of P. chrysogenum PCL501 was found to be slightly higher using glucose in comparison to lactose. In a comparative study of the growth of P. chrysogenum NCAIM 00237 strain in different carbon sources, good growth was observed with glucose, sucrose, glycerol and galactose, while growth on lactose was substantially slower (Nagy et al, 2001). Cassava shavings, in particular, yielded 1.6 -1.7 times as much mycelium as glucose and lactose.…”

Section: Discussionmentioning

confidence: 99%

Agro-waste: a potential fermentation substrate for Penicillium chrysogenum

Onygeme-Okerenta¹,

Chinedu²,

Okochi³

et al. 2009

International Journal of Biological and Chemical Sciences

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Common agro-wastes found in Lagos, Nigeria (cassava shavings, corncob, sawdust, and sugarcane pulp) were compared with glucose and lactose as fermentation substrates for Penicillium chrysogenum PCL501. Cassava shavings significantly (P<0.001) produced the highest amount of mycelia weight (0.43 ± 0.02 mg/ml) than all the other substrates. This was followed by corncob with peak mycelia weight of 0.33 ± 0.02 mg/ml. Peak mycelia weight of 0.27 ± 0.01 mg/ml was equally obtained with glucose and sugarcane pulp whereas lactose gave a slightly lower peak of 0.25 ± 0.01 mg/ml. Sawdust gave the least mycelia weight of 0.13 ± 0.01 mg/ml. Total sugar content of all the culture media steadily decreased as fungal growth progressed indicating that the organism utilized carbohydrates for growth and mycelia formation. Cultures containing cassava shavings and sawdust gave high protein peaks of 0.84 ± 0.05 and 0.65 ± 0.03 mg/ml respectively. Cultures containing corncob, glucose, lactose and sugarcane pulp yielded lower protein peaks of 0.37 ± 0.02, 0.30 ± 0.02, 0.24 ± 0.02 and 0.18 ± 0.01 mg/ml respectively. The results suggest that cassava shavings, corncob and sugarcane pulp could serve as cheap fermentation substrates for the growth of the fungus. Of all the substrates investigated, cassava shavings have the best potential to serve as substrate for fermentation by Penicillium chrysogenum PCL501.

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“…0.15 ± 0.001 h -1 and 0.14 ± 0.003 for DS54468 and 4xKO, respectively. A growth rate of 0.05 h -1 was selected for glucose-limited chemostat cultivations for both strains, as under these conditions there is acceptable production of penicillin 39 while this resembles the growth rate of P. rubens on lactose 40,41 , the carbon source used in SMP medium. A comparison of biomass concentration at several points during steady state between both strains revealed a slight increase of 6% in biomass concentration (6.59 ± 0.153 g/kg for DS54468 and 6.98 ± 0.054 g/kg for 4xKO; p=0.0034, two-tailed students t-test, n=5 for DS54468 and n= 7 for 4xKO) while dissolved oxygen tension (DOT), CO2 production, O2 consumption and base addition remained unchanged (Supplementary Information SI9)).…”

Section: Characterization Of P Rubens Devoid Of Four Bgcs Using Chemostat Cultivationmentioning

confidence: 99%

APenicillium rubensplatform strain for secondary metabolite production

Pohl

Polli

Schütze

et al. 2020

Preprint

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15We present a Penicillium rubens strain with an industrial background in which the four highly expressed 16 biosynthetic gene clusters (BGC) required to produce penicillin, roquefortine, chrysogine and fungisporin 17 were removed. This resulted in a minimal secondary metabolite background. Amino acid pools under 18 steady-state growth conditions showed reduced levels of methionine and increased intracellular aromatic 19 amino acids. Expression profiling of remaining BGC core genes and untargeted mass spectrometry did not 20 identify products from uncharacterized BGCs. This platform strain was repurposed for expression of the 21 recently identified polyketide calbistrin gene cluster and achieved high yields of decumbenone A, B and C. 22 The penicillin BGC could be restored through in vivo assembly with eight DNA segments with short 23 Page 2 of 35 overlaps. Our study paves the way for fast combinatorial assembly and expression of biosynthetic 24 pathways in a fungal strain with low endogenous secondary metabolite burden. 25 Keywords 26Platform strain, Natural products, Penicillium, Biosynthetic gene clusters, Decumbenone, Calbistrin, 27 CRISPR/Cas9, RNP 28 improvement (CSI) have led to accumulation of point mutations 18 that resulted in strains optimized for 52 high ß-lactam yield in large scale fermenters 19 and low unwanted secondary metabolite production. The 53 superior fermentation characteristics of such strains were successfully employed for the production of 54 cephalosporins 20 and, after deletion of the penicillin BGC, also for the heterologous polyketide 55 pravastatin 21 . P. rubens research has led to a full genome sequence 22 and a metabolic model 23 which makes 56 it attractive for future rational strain improvements. In addition, the efficiency of integrating multiple DNA 57 fragments into P. rubens has been increased by utilizing split-marker approaches 24 and the targetable 58 nuclease Cas9 25 . However, direct in vivo recombination for fast construction of different BGC pathway 59 combinations has not yet been demonstrated. Moreover, since the precursors for the biosynthesis of 60 penicillins, α-aminoadipic acid, L-cysteine and L-valine originate from diverse anabolic routes, a careful 61 elucidation of intracellular amino acid pools would be required to assess the impact of CSI on the flexibility 62 of the metabolism to respond to high and low amino acid demands. 63Here, we report on the construction of a P. rubens strain lacking four highly expressed secondary 64 metabolite BGCs resulting in a near to complete secondary metabolite deficient metabolome under the 65 cultivation conditions tested here. We performed genomic and transcriptome analysis, characterized its 66 amino acid profile and demonstrated its suitability for efficient BGC recombination by reconstructing the 67 Penicillin BGC (Pen-BGC) of 17kb by in vivo recombination with 8 DNA fragment with short (110 bp) 68 overlapping flanks. Finally, we utilized this new platform strain for expression of the heterologous 69 calbistrin B...

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Carbon source regulation of β-galactosidase biosynthesis inPenicillium chrysogenum

Cited by 16 publications

References 46 publications

Physiological effect of Natural Penicillin Extract on Cassava Peel Media by Penicillium chrysogenum PCL501 on E.coli Infected Wistar Rats

Physiological effect of Natural Penicillin Extract on Cassava Peel Media by Penicillium chrysogenum PCL501 on E.coli Infected Wistar Rats

Agro-waste: a potential fermentation substrate for Penicillium chrysogenum

APenicillium rubensplatform strain for secondary metabolite production

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