Carbon nanotubes quench singlet oxygen generated by photosynthetic reaction centers

Boldog, Péter; Hajdu, Kata; Magyar, Melinda; Hideg, Éva; Hernádi, Klára; Horváth, Endre; Magrez, Arnaud; Nagy, Krisztina; Varo, Gyoergy; Forró, Lászlø; Nagy, László

doi:10.1002/pssb.201300074

Cited by 12 publications

(19 citation statements)

References 17 publications

(23 reference statements)

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“…In these samples, the main part of the DPBF is distributed in the hydrophobic phase of the micelles and solubilized together with the CNT/RC fraction. This was well demonstrated experimentally by the size exclusion chromatography discussed earlier, and agrees well with model calculations when the lifetime and the diffusion path of 1 O 2 are compared [8]. Under our present experimental conditions only a small difference in the equilibrium concentration of 1 O 2 in SWCNT/RC and MWCNT/RC complexes can be recognized.…”

Section: Discussionsupporting

confidence: 91%

“…In addition to the direct energy transfer, there are several chemical reaction pathways between 1 O 2 and carbon-based nanomaterials [7]. In our earlier publication [8], we discussed the role of the rate of the forward sensitization and the backward deactivation processes on the equilibrium concentration of O 2 was used in order to determine the singlet oxygen concentration in the solution. In this assay, DPBF is converted into its colorless product DBB (o-dibenzoylbenzene) the reaction of which can be followed by measuring the absorption change at 410 nm.…”

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confidence: 99%

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Equilibrium concentration of singlet oxygen in photoreaction of reaction center/carbon nanotube bionanocomposites

Kinka

Hajdu

Magyar

et al. 2015

Phys. Status Solidi B

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The reaction center protein is the unit in the photosynthetic organisms in which the primary events of the photoelectric energy conversion take place during photosynthesis. When the photochemistry following the excitation of the reaction center by light is oversaturated there is a large probability to form reactive oxygen species (ROS, e.g., singlet oxygen ( 1 O 2 )). Because the ROS components decrease the overall yield of the photochemical energy conversion there is a considerable effort in many laboratories to find conditions to reduce these harmful compounds. The aim of our work is to create a system by using carbon nanotubes (CNTs) and RCs for efficient lightenergy conversion. The role of the 1 O 2 that destroys the RC structure is discussed. 1,3-Diphenylisobenzofuran was used to detect the concentration of the 1 O 2 . Although, 1 O 2 can be sensitized by CNTs in certain conditions, in our experiments the main sources of the 1 O 2 are the RCs. The concentration of the 1 O 2 in the equilibrium is determined by the forward sensitization and the backward deactivation processes of the components and functions of the CNT/RC composite.

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Section: Discussionsupporting

confidence: 91%

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confidence: 99%

Equilibrium concentration of singlet oxygen in photoreaction of reaction center/carbon nanotube bionanocomposites

Kinka

Hajdu

Magyar

et al. 2015

Phys. Status Solidi B

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“…74 The drop after 12 h of exposure is presumably associated either with the regulations in the intracellular glutathione (GSH), a major antioxidant that protects cells from oxidative stress 75 or the quenching effects of the internalized MWCNTs. 76 In particular, prolonged MWCNTs exposure could lead to a gradual accumulation of GSH which will eventually balance the cellular ROS oxidative levels. 77, 78 Complementary, intracellular accumulation of MWCNTs could lead to quenching of the cell-derived radicals by scavenging of the oxygen-centered molecules (i.e., HO - and O 3 + ).…”

Section: Resultsmentioning

confidence: 99%

Carbon nanotube uptake changes the biomechanical properties of human lung epithelial cells in a time-dependent manner

et al. 2015

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The toxicity of engineered nanomaterials in biological systems depends on both the nanomaterial properties and the exposure duration. Herein we used a multi-tier strategy to investigate the relationship between user-characterized multi-walled carbon nanotubes (MWCNTs) exposure duration and their induced biochemical and biomechanical effects on model human lung epithelial cells (BEAS-2B). Our results showed that exposure to MWCNTs leads to time-dependent intracellular uptake and generation of reactive oxygen species (ROS), along with time-dependent gradual changes in cellular biomechanical properties. In particular, the amount of internalized MWCNTs followed a sigmoidal curve with the majority of the MWCNTs being internalized within 6h of exposure; further, the sigmoidal uptake correlated with the changes in the oxidative levels and cellular biomechanical properties respectively. Our study provides new insights into the time-dependent induced toxicity caused by exposure to occupationally relevant doses of MWCNTs and could potentially help establish bases for early risk assessments of other nanomaterials toxicological profiles.

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“…The development of carbon nanomaterials has presented new opportunities for disease diagnosis and therapies with impact on human healthcare. In the field of diagnostics, carbon nanomaterials have performed roles in imaging and sensing, whereas their development as novel therapies has arisen mainly from their ability to support efficient drug/gene delivery systems and to promote the generation of reactive oxygen species (ROS) upon irradiation with light (e.g., cancer phototherapy) . Despite the benefits that carbon nanomaterials can provide to medicine, their intrinsic therapeutic potential, coming from their antioxidant and radical scavenging capabilities in biological systems, has not been extensively investigated.…”

Section: Introductionmentioning

confidence: 99%

“…In the field of diagnostics, carbon nanomaterials have performed rolesi ni maging [1] and sensing, [2,3] whereas their development as novel therapies has arisen mainly from their ability to support efficient drug/gened elivery systems [4,5] and to promote the generation of reactive oxygen species (ROS) upon irradiation with light (e.g.,c ancer phototherapy). [6][7][8] Despite the benefits that carbon nanomaterials can provide to medicine, their intrinsic therapeutic potential, comingf rom their antioxidant and radical scavenging capabilities in biological systems, has not been extensively investigated. The antioxidant properties of carbon-basedm aterials, such as carbon nanotubes, graphene and graphene oxide, have been broadly recognised, [9][10][11][12][13] although they have only found utility for the protection of polymericm atrices against oxidative degradation, [14][15][16][17] and not in the biomedical field due to their toxicity and low bioavailability.…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic Carbon Nanomaterials: Characterisation by Physical, Chemical, and Biological Assays

et al. 2019

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A highly hydrophilic carbon nanomaterial was generated by using an electrochemical approach, and its structure, chemical composition, redox properties, antioxidant activity and effects on cells were characterised. It was found that the nanomaterial possesses a structure dominated by sp2 carbon atoms in a non‐ordered carbon network formed by small clusters (<2 nm) of a carbonaceous material. This material has an outstanding capability for donating electrons and an unusual ability to bind metal cations. Antioxidant activity assays showed that it displays a high scavenging activity against both 2,2‐diphenyl‐1‐picrylhydrazyl and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) radicals, and a concentration‐dependent ability to protect mitochondrial lipids and intracellular thiol groups from oxidation promoted by external oxidising agents. Cell‐based assays also revealed that the nanomaterial has the ability to protect neuronal cells against oxidative damage and toxicity promoted by tert‐butyl hydroperoxide and amyloid‐β1–42 peptide. These results, combined with the attractive methodology for generating this hydrophilic carbon‐based nanomaterial, make this study the first step in addressing the therapeutic application of this new material.

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Carbon nanotubes quench singlet oxygen generated by photosynthetic reaction centers

Cited by 12 publications

References 17 publications

Equilibrium concentration of singlet oxygen in photoreaction of reaction center/carbon nanotube bionanocomposites

Equilibrium concentration of singlet oxygen in photoreaction of reaction center/carbon nanotube bionanocomposites

Carbon nanotube uptake changes the biomechanical properties of human lung epithelial cells in a time-dependent manner

Hydrophilic Carbon Nanomaterials: Characterisation by Physical, Chemical, and Biological Assays

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