Carbon Nanotubes Induce Bone Calcification by Bidirectional Interaction with Osteoblasts

Shimizu, Masayuki; Kobayashi, Yasuhiro; Mizoguchi, Takahiro; Nakamura, Hiroaki; Kawahara, Ichiro; Narita, Nobuyo; Usui, Yuki; Aoki, Katsumi; Hara, Kazuo; Haniu, Hisao; Ogihara, Nobuhide; Ishigaki, Norio; Nakamura, Kōichi; Kato, Hiroyuki; Kawakubo, Masatomo; Dohi, Yoshiko; Taruta, Seiichi; Kim, Yoong Ahm; Endo, Morinobu; Ozawa, Hiroki; Udagawa, Nobuyuki; Takahashi, Naoyuki

doi:10.1002/adma.201103832

Cited by 64 publications

(56 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Another finding was that this activation was accompanied by the deposition of hydroxyapatite around the CNTs, which was catalyzed by alkaline phosphatase (ALP) released from osteoblasts. 218 These findings demonstrated that CNTs functioning as a scaffold interact with the body to promote osteogenesis and thereby the process of bone tissue regeneration. To date, no other scaffold has interacted with the body in this way; CNTs are expected to be breakthrough materials in regenerative medicine research as well.…”

Section: Present Status Of Research Into the Application Of Cnts As Bmentioning

confidence: 94%

See 1 more Smart Citation

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

et al. 2014

Self Cite

View full text Add to dashboard Cite

Section: Present Status Of Research Into the Application Of Cnts As Bmentioning

confidence: 94%

“…466 To assess the use CNTs as a possible bone tissue regeneration scaffold, we examined in detail their effects on osteoblasts (bone-forming cells) and osteoclasts (bone-absorbing cells), as described in section 2.3.2. 217,218 …”

Section: Present Status Of Research Into In Vitro Toxicity Of Cnts Fomentioning

confidence: 99%

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

et al. 2014

Self Cite

View full text Add to dashboard Cite

“…In particular, CNTs have emerged as a promising functional nanomaterial for bone regeneration as they can promote osteogenesis of mesenchymal stem cells [5], as well as osteoblast functioning [6], and bone calcification [7]. Previous studies reported that extracellular matrix proteins adsorbed on CNTs mediate cell adhesion and subsequently remodel cell cytoskeleton [8,9].…”

Section: Introductionmentioning

confidence: 99%

In situ hybridization of carbon nanotubes with bacterial cellulose for three-dimensional hybrid bioscaffolds

Park¹,

Park²,

Jo³

et al. 2015

Biomaterials

View full text Add to dashboard Cite

“…[17][18][19] In addition, in closely related bioengineering fields, chemically modified CNTs alone or in combination with distinct composite materials have been applied to facilitate neuronal growth, cartilage, and myocardial tissue differentiation, among other applications. [20][21][22][23][24][25][26][27][28][29][30][31][32] Furthermore, CNTs in suspension have also been investigated as potential carriers for drug delivery, and have been shown to have no effect on vital functions of cells. 31,32 In the current work, we have evaluated the bone-inducing properties of carboxyl (COOH)-modified CNTs by conducting in vitro experiments on two models of progenitor cells, human fetal osteoblasts (hFOBs) and murine embryonic stem cells (mESCs), and we have further investigated bone induction in vivo in a rat model.…”

Section: Introductionmentioning

confidence: 99%

Carboxyl-modified single-wall carbon nanotubes improve bone tissue formation in vitro and repair in an in vivo rat model

Barrientos‐Durán¹,

Carpenter²,

Nieden³

et al. 2014

IJN

View full text Add to dashboard Cite

The clinical management of bone defects caused by trauma or nonunion fractures remains a challenge in orthopedic practice due to the poor integration and biocompatibility properties of the scaffold or implant material. In the current work, the osteogenic properties of carboxyl-modified single-walled carbon nanotubes (COOH–SWCNTs) were investigated in vivo and in vitro. When human preosteoblasts and murine embryonic stem cells were cultured on coverslips sprayed with COOH–SWCNTs, accelerated osteogenic differentiation was manifested by increased expression of classical bone marker genes and an increase in the secretion of osteocalcin, in addition to prior mineralization of the extracellular matrix. These results predicated COOH–SWCNTs’ use to further promote osteogenic differentiation in vivo. In contrast, both cell lines had difficulties adhering to multi-walled carbon nanotube-based scaffolds, as shown by scanning electron microscopy. While a suspension of SWCNTs caused cytotoxicity in both cell lines at levels >20 μg/mL, these levels were never achieved by release from sprayed SWCNTs, warranting the approach taken. In vivo, human allografts formed by the combination of demineralized bone matrix or cartilage particles with SWCNTs were implanted into nude rats, and ectopic bone formation was analyzed. Histological analysis of both types of implants showed high permeability and pore connectivity of the carbon nanotube-soaked implants. Numerous vascularization channels appeared in the formed tissue, additional progenitor cells were recruited, and areas of de novo ossification were found 4 weeks post-implantation. Induction of the expression of bone-related genes and the presence of secreted osteopontin protein were also confirmed by quantitative polymerase chain reaction analysis and immunofluorescence, respectively. In summary, these results are in line with prior contributions that highlight the suitability of SWCNTs as scaffolds with high bone-inducing capabilities both in vitro and in vivo, confirming them as alternatives to current bone-repair therapies.

show abstract

Carbon Nanotubes Induce Bone Calcification by Bidirectional Interaction with Osteoblasts

Cited by 64 publications

References 49 publications

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

Safe Clinical Use of Carbon Nanotubes as Innovative Biomaterials

In situ hybridization of carbon nanotubes with bacterial cellulose for three-dimensional hybrid bioscaffolds

Carboxyl-modified single-wall carbon nanotubes improve bone tissue formation in vitro and repair in an in vivo rat model

Contact Info

Product

Resources

About