Carbon Nanotubes Functionalized With Folic Acid Attached Via Biomimetic Peptide Linker

Frączyk, Justyna; Walczak, Małgorzata; Szymański, Łukasz; Kołaciński, Zbigniew; Wrzosek, H.; Majsterek, Ireneusz; Przybyłowska-Sygut, Karolina; Kamiński, Zbigniew J.

doi:10.2217/nnm-2017-0120

Cited by 16 publications

(15 citation statements)

References 59 publications

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“…In both cases, 4-toluenosulphonate 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-morpholine (DMT/NMM/TosO − ) was used as a coupling reagent [ 35 ]. In addition to high efficiency, DMT/NMM/TosO − is characterized by the easy removal of side products, thereby ensuring that there were no deposits on the functionalized nanomaterials [ 36 ] (see Figure 5 ).…”

Section: Resultsmentioning

confidence: 99%

Orthogonal Functionalization of Nanodiamond Particles after Laser Modification and Treatment with Aromatic Amine Derivatives

et al. 2018

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A laser system with a wavelength of 1064 nm was used to generate sp2 carbon on the surfaces of nanodiamond particles (NDPs). The modified by microplasma NDPs were analysed using FT-IR and Raman spectroscopy. Raman spectra confirmed that graphitization had occurred on the surfaces of the NDPs. The extent of graphitization depended on the average power used in the laser treatment process. FT-IR analysis revealed that the presence of C=C bonds in all spectra of the laser-modified powder. The characteristic peaks for olefinic bonds were much more intense than in the case of untreated powder and grew in intensity as the average laser power increased. The olefinized nanodiamond powder was further functionalized using aromatic amines via in situ generated diazonium salts. It was also found that isokinetic mixtures of structurally diverse aromatic amines containing different functional groups (acid, amine) could be used to functionalize the surfaces of the laser-modified nanoparticles leading to an amphiphilic carbon nanomaterial. This enables one-step orthogonal functionalization and opens the possibility of selectively incorporating molecules with diverse biological activities on the surfaces of NDPs. Modified NDPs with amphiphilic properties resulting from the presence carboxyl and amine groups were used to incorporate simultaneously folic acid (FA-CONH-(CH2)5-COOH) and 5(6)-carboxyfluorescein (FL-CONH-(CH2)2-NH2) derivatives on the surface of material under biocompatible procedures.

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Section: Resultsmentioning

confidence: 99%

Orthogonal Functionalization of Nanodiamond Particles after Laser Modification and Treatment with Aromatic Amine Derivatives

et al. 2018

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“…In the first stage of purification, CNTs were annealed at 450°C for 2 h in order to remove amorphous carbon. The choice of temperature resulted from the fact that, in 450°C, the amorphous carbon should be removed without losses for the CNTs [11].…”

Section: Purificationmentioning

confidence: 99%

Synthesis of functionalized carbon nanotubes for fluorescent biosensors

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Walczak

Pietrzak

et al. 2020

Nanotechnology Reviews

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Despite the development of pharmacy, there are still incurable diseases for which the medicine has not been found yet. Because many diseases are asymptomatic in their first stage of development, often early detection is the crucial factor in combating them. The article describes the process of synthesis of carbon nanotubes (CNTs) which can be useful in medical diagnostics. CNTs were synthesized by chemical vapor deposition. The obtained material was subjected to functionalization – attaching fluorescent markers. In order to check the usefulness of the obtained structures in diagnostics, their fluorescent properties were examined. The results of fourier transform infrared spectroscopy thermogravimetric analysis and scanning electron microscopy prove that, after proper functionalization, CNTs could be used as fluorescents markers.

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“…The choice of this reagent was based on its high efficiency for both classical peptide synthesis and ester synthesis in solution and on solid phase, as well as its usefulness for the functionalization of solid surfaces. DMT/NMM/TosO − has been used successfully to functionalize cellulose, alginate, chitosan and carbon nanomaterials [51,[71][72][73]. In addition to efficient acylation of nucleophilic groups in the presence of superactive triazine carboxylic acid esters, triazine coupling reagents have one more important property, crucial from the perspective of the modification of solid materials.…”

Section: Synthesismentioning

confidence: 99%

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

et al. 2020

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New materials that are as similar as possible in terms of structure and biology to the extracellular matrix (external environment) of cells are of great interest for regenerative medicine. Oligoproline and oligohydroxyproline derivatives (peptides 2–5) are potential mimetics of collagen fragments. Peptides 2–5 have been shown to be similar to the model collagen fragment (H-Gly-Hyp-Pro-Ala-Hyp-Pro-OH, 1) in terms of both their spatial structure and biological activity. In this study, peptides 2–5 were covalently bound to nonwovens based on chitosan and calcium alginate. Incorporation of the peptides was confirmed by Fourier transform -infrared (FT-IR) and zeta potential measurements. Biological studies (cell metabolic activity by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and Live/Dead assay) proved that the obtained peptide-polysaccharide conjugates were not toxic to the endothelial cell line EA.hy 926. In many cases, the conjugates had a highly affirmative influence on cell proliferation. The results of this study show that conjugates of chitosan and calcium alginate with oligoproline and oligohydroxyproline derivatives have potential for use in regenerative medicine.

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Carbon Nanotubes Functionalized With Folic Acid Attached Via Biomimetic Peptide Linker

Abstract: Conjugates of FA attached to MWNTs via peptide linkers prepared from β-alanine residues are resistant to proteolytic degradation. Viability in colon cancer cells and normal colonocytes confirmed their lack of cytotoxicity.

Cited by 16 publications

References 59 publications

Orthogonal Functionalization of Nanodiamond Particles after Laser Modification and Treatment with Aromatic Amine Derivatives

Orthogonal Functionalization of Nanodiamond Particles after Laser Modification and Treatment with Aromatic Amine Derivatives

Synthesis of functionalized carbon nanotubes for fluorescent biosensors

Conjugates of Chitosan and Calcium Alginate with Oligoproline and Oligohydroxyproline Derivatives for Potential Use in Regenerative Medicine

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