Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape

Yamashita, Kohei; Yoshioka, Yasuo; Higashisaka, Kazuma; Morishita, Yuki; Yoshida, Tokuyuki; Fujimura, Makoto; Kayamuro, Hiroyuki; Nabeshi, Hiromi; Yamashita, Takuya; Nagano, Kazuya; Abe, Yasuhiro; Kamada, Haruhiko; Kawai, Yuichi; Mayumi, Tadanori; Yoshikawa, Tomoaki; Itoh, Norio; Tsunoda, Shin-ichi; Tsutsumi, Yasuo

doi:10.1007/s10753-010-9182-7

Cited by 141 publications

(85 citation statements)

References 20 publications

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“…A single recommended exposure level was proposed (7 µg m −3 ); however, it is recognized that the diverse properties of these materials may impart a range of toxicities. As an example, in a recent comparison of inflammatory responses to different types of CNTs administered to the peritoneum of mice, long thick MWCNTs caused DNA damage and severe inflammatory effects, while similar SWCNTs caused little effect, and short thin MWCNTs had no effect (Yamashita et al, 2010). These findings suggest important differences in the biological responses of CNFs/ CNTs.…”

Section: Introductionmentioning

confidence: 99%

Exposure and Emissions Monitoring during Carbon Nanofiber Production—Part I: Elemental Carbon and Iron–Soot Aerosols

2011

The Annals of Occupational Hygiene

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Production of carbon nanofibers and nanotubes (CNFs/CNTs) and their composite products is increasing globally. High volume production may increase the exposure risks for workers who handle these materials. Though health effects data for CNFs/CNTs are limited, some studies raise serious health concerns. Given the uncertainty about their potential hazards, there is an immediate need for toxicity data and field studies to assess exposure to CNFs/CNTs. An extensive study was conducted at a facility that manufactures and processes CNFs. Filter, sorbent, cascade impactor, bulk, and microscopy samples, combined with direct-reading instruments, provided complementary information on air contaminants. Samples were analyzed for organic carbon (OC) and elemental carbon (EC), metals, and polycyclic aromatic hydrocarbons (PAHs), with EC as a measure of CNFs. Transmission electron microscopy with energy-dispersive X-ray spectroscopy also was applied. Fine/ultrafine iron-rich soot, PAHs, and carbon monoxide were production byproducts. Direct-reading instrument results were reported previously [Evans DE et al. Respirable EC area concentrations inside the facility were about 6-68 times higher than outdoors, while personal breathing zone samples were up to 170 times higher.

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Section: Introductionmentioning

confidence: 99%

Exposure and Emissions Monitoring during Carbon Nanofiber Production—Part I: Elemental Carbon and Iron–Soot Aerosols

2011

The Annals of Occupational Hygiene

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“…[15] In addition, long and thick CNTs generate significant DNA damage and potent inflammation, which can increase cancer risk and promote tumor progression. [22] To modulate their biological activity, CNTs have been modified with various polymers using covalent or noncovalent bonding depending on their specific purpose, e.g., modifying the CNTs using poly(ethyleneimine) for carrying DNA, [23] formulation of water dispersible CNTs using pyrene-end-capped or cholesterol-end-capped poly(2-methacryloyloxyethyl phosphorylcholine) and cholesterol-end-capped poly(2-dimethylamino ethyl methacrylate), [24] and enhancing cell adherence of the CNTs by applying poly(styrene-b-isobutylene-b-styrene). [25] In this study, water dispersible and biocompatible SWNTs (SWNT- CP-PDM) were obtained by the combination of a purification step and the application of wrapping materials (PDM).…”

Section: Resultsmentioning

confidence: 99%

Polyampholyte‐Wrapped Carbon Nanotubes: Preparation and Internalization by Embryonic Fibroblast Cells

Lee

Geckeler

2011

Macromol. Rapid Commun.

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The cytotoxicity and cellular uptake of carbon nanotubes (CNTs) has recently attracted considerable interest because of the issue of biosphere-nanomaterial interactions. The biocompatibility of CNTs is determined by the metal impurities in the CNTs, the size of the CNTs and the CNT dispersion states; in particular, the type of surface modifications on the CNTs affects how they interact with cells and determines their cytotoxicity and cellular uptake. In this study, biocompatible single-walled carbon nanotubes (SWNTs) wrapped with a water-soluble copolymer, poly[2-(dimethylamino)ethyl methacrylate-co-methacrylic acid] (PDM), were prepared. We report that these SWNTs have enhanced water dispersibility and cellular internalization but no significant cytotoxic activity against mouse embryonic fibroblast NIH-3T3 cells.

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“…Published data in the recent literature point to an increase of various biological responses, such as inflammatory or immune responses, after exposure to nanomaterials. [1][2][3][4][5][6][7] These biological responses have been observed and reported on from either in vitro (cell culture-based) or in vivo (whole-animal) model systems; however, more research should be conducted on relating the physicochemical features of each engineered nanomaterial to the observed biological responses. It is critical to develop computational models that not only establish quantitative relationships between different types of experimental data but can also reliably predict biological responses and/or hazards to nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

A framework for grouping nanoparticles based on their measurable characteristics

Sayes¹,

Smith²,

Ivanov³

2013

IJN

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Background: There is a need to take a broader look at nanotoxicological studies. Eventually, the field will demand that some generalizations be made. To begin to address this issue, we posed a question: are metal colloids on the nanometer-size scale a homogeneous group? In general, most people can agree that the physicochemical properties of nanomaterials can be linked and related to their induced toxicological responses. Methods: The focus of this study was to determine how a set of selected physicochemical properties of five specific metal-based colloidal materials on the nanometer-size scale -silver, copper, nickel, iron, and zinc -could be used as nanodescriptors that facilitate the grouping of these metal-based colloids. Results: The example of the framework pipeline processing provided in this paper shows the utility of specific statistical and pattern recognition techniques in grouping nanoparticles based on experimental data about their physicochemical properties. Interestingly, the results of the analyses suggest that a seemingly homogeneous group of nanoparticles could be separated into sub-groups depending on interdependencies observed in their nanodescriptors. Conclusion: These particles represent an important category of nanomaterials that are currently mass produced. Each has been reputed to induce toxicological and/or cytotoxicological effects. Here, we propose an experimental methodology coupled with mathematical and statistical modeling that can serve as a prototype for a rigorous framework that aids in the ability to group nanomaterials together and to facilitate the subsequent analysis of trends in data based on quantitative modeling of nanoparticle-specific structure-activity relationships. The computational part of the proposed framework is rather general and can be applied to other groups of nanomaterials as well.

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Carbon Nanotubes Elicit DNA Damage and Inflammatory Response Relative to Their Size and Shape

Cited by 141 publications

References 20 publications

Exposure and Emissions Monitoring during Carbon Nanofiber Production—Part I: Elemental Carbon and Iron–Soot Aerosols

Exposure and Emissions Monitoring during Carbon Nanofiber Production—Part I: Elemental Carbon and Iron–Soot Aerosols

Polyampholyte‐Wrapped Carbon Nanotubes: Preparation and Internalization by Embryonic Fibroblast Cells

A framework for grouping nanoparticles based on their measurable characteristics

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